Opiates inhibit pedunculopontine neurones in guinea pig brainstem slices

M. Serafin, A. Khateb, M. Mühlethaler

Research output: Contribution to journalArticlepeer-review


Intracellular recordings were obtained from pedunculopontine neurones in guinea-pig brainstem slices. These cells were characterized by a broad action potential, an A-like conductance and fired spontaneously in a regular manner. These neurones were inhibited by bath-application of both carbachol and serotonine at concentrations of 10-4 M. Opioid peptides induced a dose-dependent hyperpolarization and a reduction in the spontaneous firing. These latter effects could be blocked by the opiate antagonist naloxone and were direct as they persisted in presence of tetrodotoxine or high magnesium/low calcium-containing salines. They were mediated by an opiate receptor of the μ type since they were obtained with the μ-preferring enkephalin analogues FK 33-824 and DAGO, but neither with the δ nor the κ analogues such as DPLPE or U-50, 488.

Original languageEnglish
Pages (from-to)125-128
Number of pages4
JournalNeuroscience Letters
Issue number1
StatePublished - 30 Oct 1990
Externally publishedYes

Bibliographical note

Funding Information:
This work was supported by grants from the Swiss NSF (Nos. and 3.560.0.86) and the Sandoz Foundation. We thank Ms. D. Machard for excellent technical assistance.


  • Arousal
  • Cholinergic neuron
  • In vitro
  • Opiate receptor
  • Reticular formation
  • Sleep

ASJC Scopus subject areas

  • Neuroscience (all)


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