Abstract
Intracellular recordings were obtained from pedunculopontine neurones in guinea-pig brainstem slices. These cells were characterized by a broad action potential, an A-like conductance and fired spontaneously in a regular manner. These neurones were inhibited by bath-application of both carbachol and serotonine at concentrations of 10-4 M. Opioid peptides induced a dose-dependent hyperpolarization and a reduction in the spontaneous firing. These latter effects could be blocked by the opiate antagonist naloxone and were direct as they persisted in presence of tetrodotoxine or high magnesium/low calcium-containing salines. They were mediated by an opiate receptor of the μ type since they were obtained with the μ-preferring enkephalin analogues FK 33-824 and DAGO, but neither with the δ nor the κ analogues such as DPLPE or U-50, 488.
| Original language | English |
|---|---|
| Pages (from-to) | 125-128 |
| Number of pages | 4 |
| Journal | Neuroscience Letters |
| Volume | 119 |
| Issue number | 1 |
| DOIs | |
| State | Published - 30 Oct 1990 |
| Externally published | Yes |
Bibliographical note
Funding Information:This work was supported by grants from the Swiss NSF (Nos. 3.288.0.85 and 3.560.0.86) and the Sandoz Foundation. We thank Ms. D. Machard for excellent technical assistance.
Keywords
- Arousal
- Cholinergic neuron
- In vitro
- Opiate receptor
- Reticular formation
- Sleep
ASJC Scopus subject areas
- General Neuroscience
