On the genetic components of the responsiveness of Kniphofia to growth regulators and sugars

As part of an ongoing study about the genetic determinants of shoot regeneration we tested the hypothesis that the process of shoot formation and rooting in tissue culture involves groups of genetic determinants that can be discerned from each other. We used Kniphofia lines that were part of a breeding program and were crossed to produce a F1 hybrid. We used the Kniphofia propagation protocol to study the response of the parental lines to the propagation protocol and to see if we can discern some genetic insight from the F1 cross. It was found that the response to glucose was a receive type genetic response while the response to cytokinin to auxin ratio varied and dependent on the type of auxin. But in general, the response to cytokinin to auxin ratio was dominant and additive in most cases. The response to IBA in the rooting medium was semi dominant.