Light has an important role in morphogenesis of cultured plant tissue. Morphogenesis depends on both photoperiod and spectrum. Thus, the effect of light on regeneration of shoots from several petunia species leaves was studied. Sterilized leaves of petunia were placed on MS medium supplemented with 5.7 μM IAA and 2.25 μM Zeatin. Leaf tissue was exposed to varying durations of light or darkness and regeneration was recorded. Petunia hybrida cultivar R did not regenerate in darkness while P. axillaris did regenerate in darkness. P. hybrida and P. axillaris regenerated in light. P. hybrida leaves on MS regeneration medium that were exposed to light, needed a minimum of 6 days of irradiation to induced shoot regeneration. Dark-developing shoots were etiolated, which indicate that light was needed for induction of shoot morphogenesis. Crosses between P. hybrida and P. axillaris showed that there is a single dominant locus that is needed for regeneration in darkness and two loci for regeneration in light.
- Light depedent
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