Oceanobacillus chironomi sp. nov., a halotolerant and facultatively alkaliphilic species isolated from a chironomid egg mass

Dina Raats, Malka Halpern

Research output: Contribution to journalArticlepeer-review

Abstract

Chinomids (Diptera; Chironomidae) are the most abundant insect in freshwater aquatic habitats. Females of the genes Chironomus lay egg masses containing hundreds of eggs embedded in a gelatinous matrix. A bacterial strain, designated T3944DT, was isolated from a chironomid egg mass sampled from a waste-stabilization pond in northern Israel and was found to be Gram-positive, motile by peritrichous flagella, endospore-forming, halotolerant and facultatively alkaliphilic. Comparative 16S rRNA gene sequence analysis showed that strain T3944DT belonged to the genus Oceanobacillus, exhibiting the highest levels of similarity with the sequences of Oceanobacillus oncorhynchi subsp. incaldanensis DSM 16557T (94.9%), Oceanobacillus oncorhynchi subsp. oncorhynchi JCM 12661T (94.8%), Oceanobacillus iheyensis JCM 11309T (94.7%) and Oceanobacillus picturae LMG 19416 (94.5%). Strain T3944DT grew optimally at 1-3% NaCl, pH 8.5 and 37 °C. The major cellular fatty acids were anteiso-C15:0 (60.0%) and anteiso-C17:0 (12.9%)and the DNA G+C content was 38.1 mol%. On the basis of its phenotypic properties and phylogenetic distinctiveness, strain T3944DT represents a novel species in the genus Oceanobacillus, for which the name Oceanobacillus chironomi sp. nov. is proposed. The type strain is T3944DT (=LMG 23627T =DSM 18262T).

Original languageEnglish
Pages (from-to)255-259
Number of pages5
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume57
Issue number2
DOIs
StatePublished - Feb 2007

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Microbiology

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