Nutrient study for the transition from earthen sedimentation ponds to ones lined with pvc in integrated mariculture systems, what needs to be done?

John J. Lee, Damaris Rodriguez, Amir Neori, Oded Zmora, Andrew Symons, Muki Shpigel

Research output: Contribution to journalArticlepeer-review


Stable diatom populations in earthen ponds for fishpond effluent treatment supported fast rates of bivalve growth in integrated mariculture systems. However, when these ponds were lined with PVC plastic sheets to prevent seepage, the populations of benthic diatoms dwindled, and did not support, as before, a commercially acceptable rate of growth by oysters and clams. Experiments were undertaken to understand the problem and restore the diatom productivity of such ponds. Clones of Amphora luciae, A,cftenerrima, Cylindrotheca closterium, Navicula cf lineola, lenzii, N. salinicola, N. cf viminoides, and Nitzschia laevis were isolated in axenic culture from an earthen sedimentation pond. Their N, P, Si, and trace element requirements for growth in fully defined media, and in media formulated with mariculture effluent, were studied in axenic batch culture. In fully defined batch culture tests, most of the isolates achieved their highest density in media with 32 μM P, 0.7 mM N, 20 μM Fe, 10 nM-20 μM Mn, 10-20 μM Zn and Co, and ≥17.5 μM Si. Enrichment by trace elements and Si stimulated the populations of these diatoms even in media based on nutrient-enriched mariculture effluents. However, in large flow-through agnotobiotic mesocosms (700 L), only Si enrichment was needed. Si concentration >100 μM was required to promote the sustained blooms of diatoms in full-sized and commercial PVC-lined fishpond effluent treatment ponds (300 m 2, 1 m depth). Except for Si, the requirements of the diatoms for micronutrients were apparently fully satisfied by the fishpond effluents (uneaten food and fish-waste). A molar ratio of 1:1 between Si and N is necessary to sustained dense diatom populations in the pond water. It is therefore recommended to enrich plastic lined mariculture effluent treatment/sedimentation ponds with Si, if the goal is to raise bivalves as a secondary crop.

Original languageEnglish
Pages (from-to)341-353
Number of pages13
JournalJournal of Applied Phycology
Issue number5
StatePublished - Oct 2004
Externally publishedYes

Bibliographical note

Funding Information:
Supported by the Israeli Ministry for National Infrastructures (A.N., M.S. and O.Z.) and NIH/NIGMS grant 08168-22 (J.J.L.). D. R. was supported in part by NIH/GMS GM MBRS grant 08168 (to J.J.L.). We wish to thank Dr. Charles Reimer, Diatom Herbarium, Philadelphia Academy of Science, for his generous consultations on the identification of the organisms with which we worked. He gave us complete access to the extensive library and specimen collections of the Diatom Herbarium with out which we could not have completed this paper.


  • Amphora cf tenerrima
  • Amphora luciae
  • Cylindrotheca closterium
  • Navicula cf lenzii
  • Navicula cf viminoides
  • Navicula salinicola
  • Naviculacflineola
  • Nitzschia laevis
  • diatoms
  • integrated mariculture systems
  • mariculture effluent
  • microphytobenthos

ASJC Scopus subject areas

  • Aquatic Science
  • Plant Science


Dive into the research topics of 'Nutrient study for the transition from earthen sedimentation ponds to ones lined with pvc in integrated mariculture systems, what needs to be done?'. Together they form a unique fingerprint.

Cite this