TY - JOUR
T1 - Non-coding RNA as a trigger of neuropathologic disorder phenotypes in transgenic Drosophila
AU - Savvateeva-Popova, Elena
AU - Popov, Andrej
AU - Grossman, Abraham
AU - Nikitina, Ekaterina
AU - Medvedeva, Anna
AU - Molotkov, Dmitry
AU - Kamyshev, Nicholas
AU - Pyatkov, Konstantin
AU - Zatsepina, Olga
AU - Schostak, Natalya
AU - Zelentsova, Elena
AU - Pavlova, Galina
AU - Panteleev, Dmitry
AU - Riederer, Peter
AU - Evgen'ev, Michail
PY - 2008/12
Y1 - 2008/12
N2 - At most, many protein-misfolding diseases develop as environmentally induced sporadic disorders. Recent studies indicate that the dynamic interplay between a wide repertoire of noncoding RNAs and the environment play an important role in brain development and pathogenesis of brain disorders. To elucidate this new issue, novel animal models which reproduce the most prominent disease manifestations are required. For this, transgenic Drosophila strains were constructed to express small highly structured, non-coding RNA under control of a heat shock promoter. Expression of the RNA induced formation of intracellular aggregates revealed by Thioflafin T in embryonic cell culture and Congo Red in the brain of transgenic flies. Also, this strongly perturbed the brain control of locomotion monitored by the parameters of sound production and memory retention of young 5-day-old males. This novel model demonstrates that expression of non-coding RNA alone is sufficient to trigger neuropathology.
AB - At most, many protein-misfolding diseases develop as environmentally induced sporadic disorders. Recent studies indicate that the dynamic interplay between a wide repertoire of noncoding RNAs and the environment play an important role in brain development and pathogenesis of brain disorders. To elucidate this new issue, novel animal models which reproduce the most prominent disease manifestations are required. For this, transgenic Drosophila strains were constructed to express small highly structured, non-coding RNA under control of a heat shock promoter. Expression of the RNA induced formation of intracellular aggregates revealed by Thioflafin T in embryonic cell culture and Congo Red in the brain of transgenic flies. Also, this strongly perturbed the brain control of locomotion monitored by the parameters of sound production and memory retention of young 5-day-old males. This novel model demonstrates that expression of non-coding RNA alone is sufficient to trigger neuropathology.
KW - Cognitive impairments
KW - Drosophila transgenic strains
KW - Intracellular aggregates
KW - Locomotion
KW - Non-coding RNA
KW - Visuo-spatial orientation
UR - http://www.scopus.com/inward/record.url?scp=57049144903&partnerID=8YFLogxK
U2 - 10.1007/s00702-008-0078-8
DO - 10.1007/s00702-008-0078-8
M3 - Article
C2 - 18779919
AN - SCOPUS:57049144903
SN - 0300-9564
VL - 115
SP - 1629
EP - 1642
JO - Journal of Neural Transmission
JF - Journal of Neural Transmission
IS - 12
ER -