New Microsatellite Markers for the Model Coral Species Stylophora pistillata from Eilat, the Red Sea

Jacob Douek, Elad Nehoray Rachmilovitz, Baruch Rinkevich

Research output: Contribution to journalArticlepeer-review


Nineteen microsatellite loci, obtained by the whole genome sequencing approach, were developed and validated for the ‘smooth cauliflower’ coral Stylophora pistillata, a widespread Indo Pacific branching coral species. A sample size of 40 colonies collected at five reef sites along the northern Gulf of Eilat, the Red Sea, were genotyped, revealing loci reproducibly and suitable outcomes for wide applications, including population genetic studies. The 19 new microsatellite loci in this sample were composed of 4–20 alleles/locus, of which 10 microsatellites are highly polymorphic (≥10 alleles/locus). The observed and expected heterozygosity ranged between 0.289 and 0.957 (mean 0.597) and 0.101 and 0.911 (mean 0.726), respectively, and the Fixation Index (F), which also indicates the inbreeding coefficient, ranges between −0.174 and 0.569 (mean 0.207). The polymorphic information content (PIC) ranges between 0.100 and 0.904 (mean 0.699). This new set of microsatellite loci will be employed for population genetics studies as for identifying the distribution of various genotypes within S. pistillata chimeras.

Original languageEnglish
Article number244
JournalJournal of Marine Science and Engineering
Issue number2
StatePublished - Feb 2023
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2023 by the authors.


  • Gulf of Eilat
  • Stylophora pistillata
  • microsatellites markers
  • next generation sequencing
  • population genetics

ASJC Scopus subject areas

  • Civil and Structural Engineering
  • Water Science and Technology
  • Ocean Engineering


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