Nineteen microsatellite loci, obtained by the whole genome sequencing approach, were developed and validated for the ‘smooth cauliflower’ coral Stylophora pistillata, a widespread Indo Pacific branching coral species. A sample size of 40 colonies collected at five reef sites along the northern Gulf of Eilat, the Red Sea, were genotyped, revealing loci reproducibly and suitable outcomes for wide applications, including population genetic studies. The 19 new microsatellite loci in this sample were composed of 4–20 alleles/locus, of which 10 microsatellites are highly polymorphic (≥10 alleles/locus). The observed and expected heterozygosity ranged between 0.289 and 0.957 (mean 0.597) and 0.101 and 0.911 (mean 0.726), respectively, and the Fixation Index (F), which also indicates the inbreeding coefficient, ranges between −0.174 and 0.569 (mean 0.207). The polymorphic information content (PIC) ranges between 0.100 and 0.904 (mean 0.699). This new set of microsatellite loci will be employed for population genetics studies as for identifying the distribution of various genotypes within S. pistillata chimeras.
|Journal||Journal of Marine Science and Engineering|
|State||Published - Feb 2023|
Bibliographical noteFunding Information:
This research was funded by the ISF (number 3511/21)–NSFC (number 42161144006) Joint Scientific Research Program (to BR).
© 2023 by the authors.
- Gulf of Eilat
- microsatellites markers
- next generation sequencing
- population genetics
- Stylophora pistillata
ASJC Scopus subject areas
- Civil and Structural Engineering
- Water Science and Technology
- Ocean Engineering