New insights into the induction of the heat shock proteins in baculovirus infected insect cells

Yulia V. Lyupina; Olga G. Zatsepina; Anna V. Timokhova; Olga V. Orlova; Margarita V. Kostyuchenko; Svetlana N. Beljelarskaya; Michael B. Evgen'ev; Victor S. Mikhailov

doi:10.1016/j.virol.2011.09.010

New insights into the induction of the heat shock proteins in baculovirus infected insect cells

Yulia V. Lyupina, Olga G. Zatsepina, Anna V. Timokhova, Olga V. Orlova, Margarita V. Kostyuchenko, Svetlana N. Beljelarskaya, Michael B. Evgen'ev, Victor S. Mikhailov

Research output: Contribution to journal › Article › peer-review

Abstract

Eight members of the HSP/HSC70 family were identified in Spodoptera frugiperda Sf9 cells infected with Autographa californica multiple nucleopolyhedrovirus (AcMNPV) by 2D electrophoresis followed by mass spectrometry (MALDI/TOF) and a Mascot search. The family includes five HSP70s induced by AcMNPV-infection and three constitutive cognate HSC70s that remained abundant in infected cells. Confocal microscopy revealed dynamic changes in subcellular localization of HSP/HSC70s in the course of infection. At the early stages (4 to 10 hpi), a fraction of HSPs is localized in distinct speckles in cytoplasm. The speckles contained ubiquitinylated proteins suggesting that they may be aggresomes where proteins targeted by ubiquitin are sequestered or processed for proteolysis. S. frugiperda HSP90 was identified in the 2D gels by Western blotting. Its amount was unchanged during infection. A selective inhibitor of HSP90, 17-AAG, decreased the rate of viral DNA synthesis in infected cells suggesting a supportive role of HSP90 in virus replication.

Original language	English
Pages (from-to)	34-41
Number of pages	8
Journal	Virology
Volume	421
Issue number	1
DOIs	https://doi.org/10.1016/j.virol.2011.09.010
State	Published - 5 Dec 2011
Externally published	Yes

Keywords

AcMNPV
Baculovirus replication
HSC70
HSP70
HSP90
Heat shock proteins
Heat shock response
Nucleopolyhedrovirus

ASJC Scopus subject areas

Virology

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10.1016/j.virol.2011.09.010

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title = "New insights into the induction of the heat shock proteins in baculovirus infected insect cells",

abstract = "Eight members of the HSP/HSC70 family were identified in Spodoptera frugiperda Sf9 cells infected with Autographa californica multiple nucleopolyhedrovirus (AcMNPV) by 2D electrophoresis followed by mass spectrometry (MALDI/TOF) and a Mascot search. The family includes five HSP70s induced by AcMNPV-infection and three constitutive cognate HSC70s that remained abundant in infected cells. Confocal microscopy revealed dynamic changes in subcellular localization of HSP/HSC70s in the course of infection. At the early stages (4 to 10 hpi), a fraction of HSPs is localized in distinct speckles in cytoplasm. The speckles contained ubiquitinylated proteins suggesting that they may be aggresomes where proteins targeted by ubiquitin are sequestered or processed for proteolysis. S. frugiperda HSP90 was identified in the 2D gels by Western blotting. Its amount was unchanged during infection. A selective inhibitor of HSP90, 17-AAG, decreased the rate of viral DNA synthesis in infected cells suggesting a supportive role of HSP90 in virus replication.",

keywords = "AcMNPV, Baculovirus replication, HSC70, HSP70, HSP90, Heat shock proteins, Heat shock response, Nucleopolyhedrovirus",

author = "Lyupina, {Yulia V.} and Zatsepina, {Olga G.} and Timokhova, {Anna V.} and Orlova, {Olga V.} and Kostyuchenko, {Margarita V.} and Beljelarskaya, {Svetlana N.} and Evgen'ev, {Michael B.} and Mikhailov, {Victor S.}",

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doi = "10.1016/j.virol.2011.09.010",

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AU - Lyupina, Yulia V.

AU - Zatsepina, Olga G.

AU - Timokhova, Anna V.

AU - Orlova, Olga V.

AU - Kostyuchenko, Margarita V.

AU - Beljelarskaya, Svetlana N.

AU - Evgen'ev, Michael B.

AU - Mikhailov, Victor S.

PY - 2011/12/5

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N2 - Eight members of the HSP/HSC70 family were identified in Spodoptera frugiperda Sf9 cells infected with Autographa californica multiple nucleopolyhedrovirus (AcMNPV) by 2D electrophoresis followed by mass spectrometry (MALDI/TOF) and a Mascot search. The family includes five HSP70s induced by AcMNPV-infection and three constitutive cognate HSC70s that remained abundant in infected cells. Confocal microscopy revealed dynamic changes in subcellular localization of HSP/HSC70s in the course of infection. At the early stages (4 to 10 hpi), a fraction of HSPs is localized in distinct speckles in cytoplasm. The speckles contained ubiquitinylated proteins suggesting that they may be aggresomes where proteins targeted by ubiquitin are sequestered or processed for proteolysis. S. frugiperda HSP90 was identified in the 2D gels by Western blotting. Its amount was unchanged during infection. A selective inhibitor of HSP90, 17-AAG, decreased the rate of viral DNA synthesis in infected cells suggesting a supportive role of HSP90 in virus replication.

AB - Eight members of the HSP/HSC70 family were identified in Spodoptera frugiperda Sf9 cells infected with Autographa californica multiple nucleopolyhedrovirus (AcMNPV) by 2D electrophoresis followed by mass spectrometry (MALDI/TOF) and a Mascot search. The family includes five HSP70s induced by AcMNPV-infection and three constitutive cognate HSC70s that remained abundant in infected cells. Confocal microscopy revealed dynamic changes in subcellular localization of HSP/HSC70s in the course of infection. At the early stages (4 to 10 hpi), a fraction of HSPs is localized in distinct speckles in cytoplasm. The speckles contained ubiquitinylated proteins suggesting that they may be aggresomes where proteins targeted by ubiquitin are sequestered or processed for proteolysis. S. frugiperda HSP90 was identified in the 2D gels by Western blotting. Its amount was unchanged during infection. A selective inhibitor of HSP90, 17-AAG, decreased the rate of viral DNA synthesis in infected cells suggesting a supportive role of HSP90 in virus replication.

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KW - Baculovirus replication

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KW - HSP90

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KW - Heat shock response

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New insights into the induction of the heat shock proteins in baculovirus infected insect cells

Abstract

Keywords

ASJC Scopus subject areas

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