Aegilops tauschii, the wild progenitor of wheat D-genome and a valuable germplasm for wheat improvement, has a wide natural distribution from eastern Turkey to China. However, the phylogenetic relationship and dispersion history of Ae. tauschii in China has not been scientifically clarified. In this study, we genotyped 208 accessions (with 104 in China) using ddRAD sequencing and 55K SNP array, and classified the population into six sublineages. Three possible spreading routes or events were identified, resulting in specific distribution patterns, with four sublineages found in Xinjiang, one in Qinghai, two in Shaanxi and one in Henan. We also established the correlation of SNP-based, karyotype-based and spike-morphology-based techniques to demonstrate the internal classification of Ae. tauschii, and developed consensus dataset with 1245 putative accessions by merging data previously published. Our analysis suggested that eight inter-lineage accessions could be assigned to the putative Lineage 3 and these accessions would help to conserve the genetic diversity of the species. By developing the consensus phylogenetic relationships of Ae. tauschii, our work validated the hypothesis on the dispersal history of Ae. tauschii in China, and contributed to the efficient and comprehensive germplasm-mining of the species.
Bibliographical noteFunding Information:
We are grateful to Dr. Le Wang (Jilin University) for the improvement with the manuscript. This work was supported in part by the National Natural Science Foundation of China ( 32001492 ), the Ministry of Agriculture of China ( 2016ZX08009 ) and the Natural Science Foundation of Henan Province ( 202300410053 ).
We are grateful to Dr. Le Wang (Jilin University) for the improvement with the manuscript. This work was supported by the National Natural Science Foundation of China (32001492), the Ministry of Agriculture of China (2016ZX08009) and the Natural Science Foundation of Henan (202300410053).
© 2021 The Authors
- Aegilops tauschii
- Consensus dataset
- Dispersion history
- Genetic diversity
- Phylogenetic relationship
ASJC Scopus subject areas
- Molecular Biology