Monoclonal antibody specific to urochordate Botryllus schlosseri pyloric gland

Ziva Lapidot; Guy Paz; Baruch Rinkevich

doi:10.1007/s10126-002-0087-9

Monoclonal antibody specific to urochordate Botryllus schlosseri pyloric gland

Research output: Contribution to journal › Article › peer-review

Abstract

We describe here the development of a new hybridoma cell line, CF12F6, that produces a specific antibody to Botryllus schlosseri (a colonial tunicate). The monoclonal antibody was isotyped as IgG1 (by enzyme-linked immunosorbent assay), and the cellular localization of the antigenic epitope that reacts specifically with CF12F6 was confined to the cells of the pyloric gland of the zooid (by immunohistochemistry). The pyloric gland participates in osmoregulation, digestion, glycogen storage, and various other secretion functions that will be studied further by the use of monoclonal antibody CF12F6, the first in botryllid ascidians that recognizes cells of a whole, single organ.

Original language	English
Pages (from-to)	388-394
Number of pages	7
Journal	Marine Biotechnology
Volume	5
Issue number	4
DOIs	https://doi.org/10.1007/s10126-002-0087-9
State	Published - Jul 2003
Externally published	Yes

Keywords

Ascidians
Botryllinae
Monoclonal
Pyloric gland
Zooid

ASJC Scopus subject areas

Biotechnology
Aquatic Science

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10.1007/s10126-002-0087-9

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title = "Monoclonal antibody specific to urochordate Botryllus schlosseri pyloric gland",

abstract = "We describe here the development of a new hybridoma cell line, CF12F6, that produces a specific antibody to Botryllus schlosseri (a colonial tunicate). The monoclonal antibody was isotyped as IgG1 (by enzyme-linked immunosorbent assay), and the cellular localization of the antigenic epitope that reacts specifically with CF12F6 was confined to the cells of the pyloric gland of the zooid (by immunohistochemistry). The pyloric gland participates in osmoregulation, digestion, glycogen storage, and various other secretion functions that will be studied further by the use of monoclonal antibody CF12F6, the first in botryllid ascidians that recognizes cells of a whole, single organ.",

keywords = "Ascidians, Botryllinae, Monoclonal, Pyloric gland, Zooid",

author = "Ziva Lapidot and Guy Paz and Baruch Rinkevich",

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AU - Lapidot, Ziva

AU - Paz, Guy

AU - Rinkevich, Baruch

PY - 2003/7

Y1 - 2003/7

N2 - We describe here the development of a new hybridoma cell line, CF12F6, that produces a specific antibody to Botryllus schlosseri (a colonial tunicate). The monoclonal antibody was isotyped as IgG1 (by enzyme-linked immunosorbent assay), and the cellular localization of the antigenic epitope that reacts specifically with CF12F6 was confined to the cells of the pyloric gland of the zooid (by immunohistochemistry). The pyloric gland participates in osmoregulation, digestion, glycogen storage, and various other secretion functions that will be studied further by the use of monoclonal antibody CF12F6, the first in botryllid ascidians that recognizes cells of a whole, single organ.

AB - We describe here the development of a new hybridoma cell line, CF12F6, that produces a specific antibody to Botryllus schlosseri (a colonial tunicate). The monoclonal antibody was isotyped as IgG1 (by enzyme-linked immunosorbent assay), and the cellular localization of the antigenic epitope that reacts specifically with CF12F6 was confined to the cells of the pyloric gland of the zooid (by immunohistochemistry). The pyloric gland participates in osmoregulation, digestion, glycogen storage, and various other secretion functions that will be studied further by the use of monoclonal antibody CF12F6, the first in botryllid ascidians that recognizes cells of a whole, single organ.

KW - Ascidians

KW - Botryllinae

KW - Monoclonal

KW - Pyloric gland

KW - Zooid

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M3 - Article

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AN - SCOPUS:0142120621

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JO - Marine Biotechnology

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IS - 4

ER -

Monoclonal antibody specific to urochordate Botryllus schlosseri pyloric gland

Abstract

Keywords

ASJC Scopus subject areas

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