Molecular identification of a new powdery mildew resistance gene Pm41 on chromosome 3BL derived from wild emmer (Triticum turgidum var. dicoccoides)

Genqiao Li; Tilin Fang; Hongtao Zhang; Chaojie Xie; Hongjie Li; Tsomin Yang; Eviatar Nevo; Tzion Fahima; Qixin Sun; Zhiyong Liu

doi:10.1007/s00122-009-1061-y

Molecular identification of a new powdery mildew resistance gene Pm41 on chromosome 3BL derived from wild emmer (Triticum turgidum var. dicoccoides)

Genqiao Li, Tilin Fang, Hongtao Zhang, Chaojie Xie, Hongjie Li, Tsomin Yang, Eviatar Nevo, Tzion Fahima, Qixin Sun, Zhiyong Liu

Department of Evolutionary and Environmental Biology

Research output: Contribution to journal › Article › peer-review

Abstract

Powdery mildew caused by Blumeria graminis f. sp. tritici is an important wheat disease in China and other parts of the world. Wild emmer (Triticum turgidum var. dicoccoides) is the immediate progenitor of cultivated tetraploid and hexaploid wheats and thus an important resource for wheat improvement. Wild emmer accession IW2 collected from Mount Hermon, Israel, is highly resistant to powdery mildew at the seedling and adult plant stages. Genetic analysis using an F₂ segregating population and F_2:3 families, derived from a cross between susceptible durum cultivar Langdon and wild emmer accession IW2, indicated that a single dominant gene was responsible for the resistance of IW2. Bulked segregant and molecular marker analyses detected that six polymorphic SSR, one ISBP, and three EST-STS markers on chromosome 3BL bin 0.63-1.00 were linked to the resistance gene. Allelic variations of resistance-linked EST-STS marker BE489472 revealed that the allele was present only in wild emmer but absent in common wheat. Segregation distortion was observed for the powdery mildew resistance allele and its linked SSR markers with preferential transmission of Langdon alleles over IW2 alleles. The resistance gene was introgressed into common wheat by backcrossing and marker-assisted selection. Since no designated powdery mildew resistance gene has been found on chromosome 3BL, the resistance gene derived from wild emmer accession IW2 appears to be new one and was consequently designated Pm41.

Original language	English
Pages (from-to)	531-539
Number of pages	9
Journal	Theoretical And Applied Genetics
Volume	119
Issue number	3
DOIs	https://doi.org/10.1007/s00122-009-1061-y
State	Published - Aug 2009

Bibliographical note

Funding Information:
The Chinese Spring aneulpoid and deletion lines used in this study were originally provided by Drs. WJ Raupp and BS Gill of Wheat Genetics Resource Centre, Kansas State University, USA. The authors are grateful to Dr. R McIntosh of University of Sydney, Australia, for his improvement of the manuscript. This work was financially supported by the National Fund for Distinguished Young Scholars (30425039), National Natural Science Foundation of China (30571151, 30771341), Beijing Natural Science Foundation (6061003), and the State High Tech Programs (2006AA100102, 2006AA10Z1E9, 2006AA10Z1C4, 2006AA10A104, and 2006BAD01A02), State Transgenic Project (2008ZX08009-002), the Program of Introducing Talents of Discipline to Universities (111-2-03), and the Program for Changjiang Scholars and Innovative Research Teams in Universities.

ASJC Scopus subject areas

Biotechnology
Agronomy and Crop Science
Genetics

UN SDGs

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10.1007/s00122-009-1061-y

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title = "Molecular identification of a new powdery mildew resistance gene Pm41 on chromosome 3BL derived from wild emmer (Triticum turgidum var. dicoccoides)",

abstract = "Powdery mildew caused by Blumeria graminis f. sp. tritici is an important wheat disease in China and other parts of the world. Wild emmer (Triticum turgidum var. dicoccoides) is the immediate progenitor of cultivated tetraploid and hexaploid wheats and thus an important resource for wheat improvement. Wild emmer accession IW2 collected from Mount Hermon, Israel, is highly resistant to powdery mildew at the seedling and adult plant stages. Genetic analysis using an F2 segregating population and F2:3 families, derived from a cross between susceptible durum cultivar Langdon and wild emmer accession IW2, indicated that a single dominant gene was responsible for the resistance of IW2. Bulked segregant and molecular marker analyses detected that six polymorphic SSR, one ISBP, and three EST-STS markers on chromosome 3BL bin 0.63-1.00 were linked to the resistance gene. Allelic variations of resistance-linked EST-STS marker BE489472 revealed that the allele was present only in wild emmer but absent in common wheat. Segregation distortion was observed for the powdery mildew resistance allele and its linked SSR markers with preferential transmission of Langdon alleles over IW2 alleles. The resistance gene was introgressed into common wheat by backcrossing and marker-assisted selection. Since no designated powdery mildew resistance gene has been found on chromosome 3BL, the resistance gene derived from wild emmer accession IW2 appears to be new one and was consequently designated Pm41.",

author = "Genqiao Li and Tilin Fang and Hongtao Zhang and Chaojie Xie and Hongjie Li and Tsomin Yang and Eviatar Nevo and Tzion Fahima and Qixin Sun and Zhiyong Liu",

note = "Funding Information: The Chinese Spring aneulpoid and deletion lines used in this study were originally provided by Drs. WJ Raupp and BS Gill of Wheat Genetics Resource Centre, Kansas State University, USA. The authors are grateful to Dr. R McIntosh of University of Sydney, Australia, for his improvement of the manuscript. This work was financially supported by the National Fund for Distinguished Young Scholars (30425039), National Natural Science Foundation of China (30571151, 30771341), Beijing Natural Science Foundation (6061003), and the State High Tech Programs (2006AA100102, 2006AA10Z1E9, 2006AA10Z1C4, 2006AA10A104, and 2006BAD01A02), State Transgenic Project (2008ZX08009-002), the Program of Introducing Talents of Discipline to Universities (111-2-03), and the Program for Changjiang Scholars and Innovative Research Teams in Universities.",

year = "2009",

month = aug,

doi = "10.1007/s00122-009-1061-y",

language = "English",

volume = "119",

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journal = "Theoretical And Applied Genetics",

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T1 - Molecular identification of a new powdery mildew resistance gene Pm41 on chromosome 3BL derived from wild emmer (Triticum turgidum var. dicoccoides)

AU - Li, Genqiao

AU - Fang, Tilin

AU - Zhang, Hongtao

AU - Xie, Chaojie

AU - Li, Hongjie

AU - Yang, Tsomin

AU - Nevo, Eviatar

AU - Fahima, Tzion

AU - Sun, Qixin

AU - Liu, Zhiyong

N1 - Funding Information: The Chinese Spring aneulpoid and deletion lines used in this study were originally provided by Drs. WJ Raupp and BS Gill of Wheat Genetics Resource Centre, Kansas State University, USA. The authors are grateful to Dr. R McIntosh of University of Sydney, Australia, for his improvement of the manuscript. This work was financially supported by the National Fund for Distinguished Young Scholars (30425039), National Natural Science Foundation of China (30571151, 30771341), Beijing Natural Science Foundation (6061003), and the State High Tech Programs (2006AA100102, 2006AA10Z1E9, 2006AA10Z1C4, 2006AA10A104, and 2006BAD01A02), State Transgenic Project (2008ZX08009-002), the Program of Introducing Talents of Discipline to Universities (111-2-03), and the Program for Changjiang Scholars and Innovative Research Teams in Universities.

PY - 2009/8

Y1 - 2009/8

N2 - Powdery mildew caused by Blumeria graminis f. sp. tritici is an important wheat disease in China and other parts of the world. Wild emmer (Triticum turgidum var. dicoccoides) is the immediate progenitor of cultivated tetraploid and hexaploid wheats and thus an important resource for wheat improvement. Wild emmer accession IW2 collected from Mount Hermon, Israel, is highly resistant to powdery mildew at the seedling and adult plant stages. Genetic analysis using an F2 segregating population and F2:3 families, derived from a cross between susceptible durum cultivar Langdon and wild emmer accession IW2, indicated that a single dominant gene was responsible for the resistance of IW2. Bulked segregant and molecular marker analyses detected that six polymorphic SSR, one ISBP, and three EST-STS markers on chromosome 3BL bin 0.63-1.00 were linked to the resistance gene. Allelic variations of resistance-linked EST-STS marker BE489472 revealed that the allele was present only in wild emmer but absent in common wheat. Segregation distortion was observed for the powdery mildew resistance allele and its linked SSR markers with preferential transmission of Langdon alleles over IW2 alleles. The resistance gene was introgressed into common wheat by backcrossing and marker-assisted selection. Since no designated powdery mildew resistance gene has been found on chromosome 3BL, the resistance gene derived from wild emmer accession IW2 appears to be new one and was consequently designated Pm41.

AB - Powdery mildew caused by Blumeria graminis f. sp. tritici is an important wheat disease in China and other parts of the world. Wild emmer (Triticum turgidum var. dicoccoides) is the immediate progenitor of cultivated tetraploid and hexaploid wheats and thus an important resource for wheat improvement. Wild emmer accession IW2 collected from Mount Hermon, Israel, is highly resistant to powdery mildew at the seedling and adult plant stages. Genetic analysis using an F2 segregating population and F2:3 families, derived from a cross between susceptible durum cultivar Langdon and wild emmer accession IW2, indicated that a single dominant gene was responsible for the resistance of IW2. Bulked segregant and molecular marker analyses detected that six polymorphic SSR, one ISBP, and three EST-STS markers on chromosome 3BL bin 0.63-1.00 were linked to the resistance gene. Allelic variations of resistance-linked EST-STS marker BE489472 revealed that the allele was present only in wild emmer but absent in common wheat. Segregation distortion was observed for the powdery mildew resistance allele and its linked SSR markers with preferential transmission of Langdon alleles over IW2 alleles. The resistance gene was introgressed into common wheat by backcrossing and marker-assisted selection. Since no designated powdery mildew resistance gene has been found on chromosome 3BL, the resistance gene derived from wild emmer accession IW2 appears to be new one and was consequently designated Pm41.

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Molecular identification of a new powdery mildew resistance gene Pm41 on chromosome 3BL derived from wild emmer (Triticum turgidum var. dicoccoides)

Abstract

Bibliographical note

ASJC Scopus subject areas

UN SDGs

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