Molecular cloning, functional verification, and evolution of TmPm3, the powdery mildew resistance gene of Triticum monococcum L

C. Z. Zhao, Y. H. Li, H. T. Dong, M. M. Geng, W. H. Liu, F. Li, Z. F. Ni, X. J. Wang, C. J. Xie, Q. X. Sun

Research output: Contribution to journalArticlepeer-review

Abstract

Powdery mildew (Pm) is one of the most harmful diseases in wheat. Three Pm-resistance genes, Pm3, Pm21, and Pm8, have been cloned but most Pm3/Pm8 alleles have lost their resistance to Pm in hexaploid wheat. In this study, a new Pm3 homolog gene (TmPm3) was isolated from Triticum monococcum L. using a homology-based cloning strategy, being the first report of a functional Pm3 homolog gene from a diploid wheat species. The transient expression of TmPm3 in leaf epidermal cells showed that over-expressed TmPm3 could significantly inhibit the penetration of Blumeria graminis f. sp tritici conidia spores and the formation of haustoria. Sequence analysis of Pm3 alleles shed new light on the evolution of Pm3 genes, providing a better understanding of the molecular basis of disease resistance. This study also suggested that homology-based cloning of resistance genes is a feasible method for the isolation of functional resistance genes from wheat germplasm.

Original languageEnglish
Article number15028056
JournalGenetics and Molecular Research
Volume15
Issue number2
DOIs
StatePublished - 25 Apr 2016
Externally publishedYes

Bibliographical note

Publisher Copyright:
© FUNPEC-RP.

Keywords

  • Plant resistance breeding
  • Pm3
  • Powdery mildew
  • Resistance gene
  • Triticum monococcum
  • Wheat

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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