Molecular characterization of a novel powdery mildew resistance gene Pm30 in wheat originating from wild emmer

Zhiyong Liu; Qixin Sun; Zhongfu Ni; Eviatar Nevo; Tsomin Yang

doi:10.1023/A:1014471113511

Molecular characterization of a novel powdery mildew resistance gene Pm30 in wheat originating from wild emmer

Zhiyong Liu, Qixin Sun, Zhongfu Ni, Eviatar Nevo, Tsomin Yang

Department of Evolutionary and Environmental Biology

Research output: Contribution to journal › Article › peer-review

Abstract

Powdery mildew caused by Erysiphe graminis f. sp. tritici is one of the most important wheat diseases in many regions of the world. A powdery mildew resistance gene, originating from wild emmer wheat (Triticum dicoccoides) accession 'C20', from Rosh Pinna, Israel, was successfully transferred to hexaploid wheat through crossing and backcrossing. Genetic analysis indicated that a single dominant gene controls the powdery mildew resistance at the seedling stage. Segregating BC₁ F₂ progenies of the cross 87-1/C20//2*8866 were used for bulked segregant analysis (BSA). The PCR approach was used to generate polymorphic DNA fragments between the resistant and susceptible DNA pools by use of 10-mer random primers, STS primers, and wheat microsatellite primers. Three markers, Xgwm159/430, Xgwm159/460, and Xgwm159/500, were found to be linked to the resistance gene. After evaluating the polymorphic markers in two segregating populations, the distance between the markers and the mildew resistance gene was estimated to be 5-6 cM. By means of Chinese Spring nullisomic-tetrasomics and ditelosomics, the polymorphic markers and the resistance gene were assigned to chromosome arm 5BS and were physically mapped on the gene rich regions of fragment length (FL) 0.41-0.43 by Chinese Spring deletion lines. As no powdery mildew resistance gene has been reported on chromosome arm 5BS, the mildew resistance gene originating from C20 should be a new gene and is designated Pm30.

Original language	English
Pages (from-to)	21-29
Number of pages	9
Journal	Euphytica
Volume	123
Issue number	1
DOIs	https://doi.org/10.1023/A:1014471113511
State	Published - 2002

Bibliographical note

Funding Information:
We are grateful to Dr M.S. Röder (Institut für Pflanz-engenetik und Kulturpflanzenforschung, Gatersleben, Germany), and Dr M.D. Gale (John Innes Centre, UK), for supplying wheat microsatellite primers, and to Dr T.K. Blake and Dr L.E. Talbert (Montana State University, USA) for barley and wheat STS primers. We also thank Drs W.J. Raupp and B.S. Gill (Wheat Genetics Resource Center, Kansas State University, USA) for providing seeds of Chinese Spring deletion lines of chromosome arm 5BS. Prof. E. Nevo thanks the Israel Discount Bank Chair of Evolutionary Biology, and the Ancell-Teicher Research Foundation for Genetics and Molecular Evolution. The authors thank Prof R.A. McIntosh and Prof F.J. Zeller for their comments and critical reviews of the manuscript. This work was financially supported by the National Natural Science Foundation of China (Grant No. 39670508).

Keywords

Blumeria graminis
Erysiphe graminis f. sp. tritici
Microsatellite
Physical mapping
Powdery mildew resistance
Triticum dicoccoides
Wild emmer

ASJC Scopus subject areas

Agronomy and Crop Science
Genetics
Plant Science
Horticulture

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@article{debe79c14ef44f56a9fffeb785bda8fe,

title = "Molecular characterization of a novel powdery mildew resistance gene Pm30 in wheat originating from wild emmer",

abstract = "Powdery mildew caused by Erysiphe graminis f. sp. tritici is one of the most important wheat diseases in many regions of the world. A powdery mildew resistance gene, originating from wild emmer wheat (Triticum dicoccoides) accession 'C20', from Rosh Pinna, Israel, was successfully transferred to hexaploid wheat through crossing and backcrossing. Genetic analysis indicated that a single dominant gene controls the powdery mildew resistance at the seedling stage. Segregating BC1 F2 progenies of the cross 87-1/C20//2*8866 were used for bulked segregant analysis (BSA). The PCR approach was used to generate polymorphic DNA fragments between the resistant and susceptible DNA pools by use of 10-mer random primers, STS primers, and wheat microsatellite primers. Three markers, Xgwm159/430, Xgwm159/460, and Xgwm159/500, were found to be linked to the resistance gene. After evaluating the polymorphic markers in two segregating populations, the distance between the markers and the mildew resistance gene was estimated to be 5-6 cM. By means of Chinese Spring nullisomic-tetrasomics and ditelosomics, the polymorphic markers and the resistance gene were assigned to chromosome arm 5BS and were physically mapped on the gene rich regions of fragment length (FL) 0.41-0.43 by Chinese Spring deletion lines. As no powdery mildew resistance gene has been reported on chromosome arm 5BS, the mildew resistance gene originating from C20 should be a new gene and is designated Pm30.",

keywords = "Blumeria graminis, Erysiphe graminis f. sp. tritici, Microsatellite, Physical mapping, Powdery mildew resistance, Triticum dicoccoides, Wild emmer",

author = "Zhiyong Liu and Qixin Sun and Zhongfu Ni and Eviatar Nevo and Tsomin Yang",

note = "Funding Information: We are grateful to Dr M.S. R{\"o}der (Institut f{\"u}r Pflanz-engenetik und Kulturpflanzenforschung, Gatersleben, Germany), and Dr M.D. Gale (John Innes Centre, UK), for supplying wheat microsatellite primers, and to Dr T.K. Blake and Dr L.E. Talbert (Montana State University, USA) for barley and wheat STS primers. We also thank Drs W.J. Raupp and B.S. Gill (Wheat Genetics Resource Center, Kansas State University, USA) for providing seeds of Chinese Spring deletion lines of chromosome arm 5BS. Prof. E. Nevo thanks the Israel Discount Bank Chair of Evolutionary Biology, and the Ancell-Teicher Research Foundation for Genetics and Molecular Evolution. The authors thank Prof R.A. McIntosh and Prof F.J. Zeller for their comments and critical reviews of the manuscript. This work was financially supported by the National Natural Science Foundation of China (Grant No. 39670508).",

year = "2002",

doi = "10.1023/A:1014471113511",

language = "English",

volume = "123",

pages = "21--29",

journal = "Euphytica",

issn = "0014-2336",

publisher = "Springer Science and Business Media B.V.",

number = "1",

}

TY - JOUR

T1 - Molecular characterization of a novel powdery mildew resistance gene Pm30 in wheat originating from wild emmer

AU - Liu, Zhiyong

AU - Sun, Qixin

AU - Ni, Zhongfu

AU - Nevo, Eviatar

AU - Yang, Tsomin

N1 - Funding Information: We are grateful to Dr M.S. Röder (Institut für Pflanz-engenetik und Kulturpflanzenforschung, Gatersleben, Germany), and Dr M.D. Gale (John Innes Centre, UK), for supplying wheat microsatellite primers, and to Dr T.K. Blake and Dr L.E. Talbert (Montana State University, USA) for barley and wheat STS primers. We also thank Drs W.J. Raupp and B.S. Gill (Wheat Genetics Resource Center, Kansas State University, USA) for providing seeds of Chinese Spring deletion lines of chromosome arm 5BS. Prof. E. Nevo thanks the Israel Discount Bank Chair of Evolutionary Biology, and the Ancell-Teicher Research Foundation for Genetics and Molecular Evolution. The authors thank Prof R.A. McIntosh and Prof F.J. Zeller for their comments and critical reviews of the manuscript. This work was financially supported by the National Natural Science Foundation of China (Grant No. 39670508).

PY - 2002

Y1 - 2002

N2 - Powdery mildew caused by Erysiphe graminis f. sp. tritici is one of the most important wheat diseases in many regions of the world. A powdery mildew resistance gene, originating from wild emmer wheat (Triticum dicoccoides) accession 'C20', from Rosh Pinna, Israel, was successfully transferred to hexaploid wheat through crossing and backcrossing. Genetic analysis indicated that a single dominant gene controls the powdery mildew resistance at the seedling stage. Segregating BC1 F2 progenies of the cross 87-1/C20//2*8866 were used for bulked segregant analysis (BSA). The PCR approach was used to generate polymorphic DNA fragments between the resistant and susceptible DNA pools by use of 10-mer random primers, STS primers, and wheat microsatellite primers. Three markers, Xgwm159/430, Xgwm159/460, and Xgwm159/500, were found to be linked to the resistance gene. After evaluating the polymorphic markers in two segregating populations, the distance between the markers and the mildew resistance gene was estimated to be 5-6 cM. By means of Chinese Spring nullisomic-tetrasomics and ditelosomics, the polymorphic markers and the resistance gene were assigned to chromosome arm 5BS and were physically mapped on the gene rich regions of fragment length (FL) 0.41-0.43 by Chinese Spring deletion lines. As no powdery mildew resistance gene has been reported on chromosome arm 5BS, the mildew resistance gene originating from C20 should be a new gene and is designated Pm30.

AB - Powdery mildew caused by Erysiphe graminis f. sp. tritici is one of the most important wheat diseases in many regions of the world. A powdery mildew resistance gene, originating from wild emmer wheat (Triticum dicoccoides) accession 'C20', from Rosh Pinna, Israel, was successfully transferred to hexaploid wheat through crossing and backcrossing. Genetic analysis indicated that a single dominant gene controls the powdery mildew resistance at the seedling stage. Segregating BC1 F2 progenies of the cross 87-1/C20//2*8866 were used for bulked segregant analysis (BSA). The PCR approach was used to generate polymorphic DNA fragments between the resistant and susceptible DNA pools by use of 10-mer random primers, STS primers, and wheat microsatellite primers. Three markers, Xgwm159/430, Xgwm159/460, and Xgwm159/500, were found to be linked to the resistance gene. After evaluating the polymorphic markers in two segregating populations, the distance between the markers and the mildew resistance gene was estimated to be 5-6 cM. By means of Chinese Spring nullisomic-tetrasomics and ditelosomics, the polymorphic markers and the resistance gene were assigned to chromosome arm 5BS and were physically mapped on the gene rich regions of fragment length (FL) 0.41-0.43 by Chinese Spring deletion lines. As no powdery mildew resistance gene has been reported on chromosome arm 5BS, the mildew resistance gene originating from C20 should be a new gene and is designated Pm30.

KW - Blumeria graminis

KW - Erysiphe graminis f. sp. tritici

KW - Microsatellite

KW - Physical mapping

KW - Powdery mildew resistance

KW - Triticum dicoccoides

KW - Wild emmer

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U2 - 10.1023/A:1014471113511

DO - 10.1023/A:1014471113511

M3 - Article

AN - SCOPUS:0036125935

SN - 0014-2336

VL - 123

SP - 21

EP - 29

JO - Euphytica

JF - Euphytica

IS - 1

ER -

Molecular characterization of a novel powdery mildew resistance gene Pm30 in wheat originating from wild emmer

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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