Molecular characterization of a novel powdery mildew resistance gene Pm30 in wheat originating from wild emmer

Zhiyong Liu, Qixin Sun, Zhongfu Ni, Eviatar Nevo, Tsomin Yang

Research output: Contribution to journalArticlepeer-review


Powdery mildew caused by Erysiphe graminis f. sp. tritici is one of the most important wheat diseases in many regions of the world. A powdery mildew resistance gene, originating from wild emmer wheat (Triticum dicoccoides) accession 'C20', from Rosh Pinna, Israel, was successfully transferred to hexaploid wheat through crossing and backcrossing. Genetic analysis indicated that a single dominant gene controls the powdery mildew resistance at the seedling stage. Segregating BC1 F2 progenies of the cross 87-1/C20//2*8866 were used for bulked segregant analysis (BSA). The PCR approach was used to generate polymorphic DNA fragments between the resistant and susceptible DNA pools by use of 10-mer random primers, STS primers, and wheat microsatellite primers. Three markers, Xgwm159/430, Xgwm159/460, and Xgwm159/500, were found to be linked to the resistance gene. After evaluating the polymorphic markers in two segregating populations, the distance between the markers and the mildew resistance gene was estimated to be 5-6 cM. By means of Chinese Spring nullisomic-tetrasomics and ditelosomics, the polymorphic markers and the resistance gene were assigned to chromosome arm 5BS and were physically mapped on the gene rich regions of fragment length (FL) 0.41-0.43 by Chinese Spring deletion lines. As no powdery mildew resistance gene has been reported on chromosome arm 5BS, the mildew resistance gene originating from C20 should be a new gene and is designated Pm30.

Original languageEnglish
Pages (from-to)21-29
Number of pages9
Issue number1
StatePublished - 2002

Bibliographical note

Funding Information:
We are grateful to Dr M.S. Röder (Institut für Pflanz-engenetik und Kulturpflanzenforschung, Gatersleben, Germany), and Dr M.D. Gale (John Innes Centre, UK), for supplying wheat microsatellite primers, and to Dr T.K. Blake and Dr L.E. Talbert (Montana State University, USA) for barley and wheat STS primers. We also thank Drs W.J. Raupp and B.S. Gill (Wheat Genetics Resource Center, Kansas State University, USA) for providing seeds of Chinese Spring deletion lines of chromosome arm 5BS. Prof. E. Nevo thanks the Israel Discount Bank Chair of Evolutionary Biology, and the Ancell-Teicher Research Foundation for Genetics and Molecular Evolution. The authors thank Prof R.A. McIntosh and Prof F.J. Zeller for their comments and critical reviews of the manuscript. This work was financially supported by the National Natural Science Foundation of China (Grant No. 39670508).


  • Blumeria graminis
  • Erysiphe graminis f. sp. tritici
  • Microsatellite
  • Physical mapping
  • Powdery mildew resistance
  • Triticum dicoccoides
  • Wild emmer

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science
  • Horticulture


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