Mitochondrial dna variability in natural populations of hawaiian drosophila. I. methods and levels of variability in d. silvestris and d. heteroneura populations

Rob Desalle; L. Val Giddings; Alan R. Templeton

doi:10.1038/hdy.1986.11

Mitochondrial dna variability in natural populations of hawaiian drosophila. I. methods and levels of variability in d. silvestris and d. heteroneura populations

Rob Desalle, L. Val Giddings, Alan R. Templeton

Research output: Contribution to journal › Article › peer-review

Abstract

We describe techniques by which mitochondrial DNA (mtDNA) restriction site information can be obtained for up to 16 different restriction endonucleases on individual Hawaiian Drosophila, in particular D. silvestris and D. heteroneura. We have constructed mtDNA restriction site maps for a total of forty-eight wild caught genomes of both species, from seven major collecting sites on the island of Hawaii. Levels of variability are, in general, high in D. silvestris (p of Ewens et al., 1981 =0·0486) and lower in D. heteroneura (p =0·0327). Measures of population subdivision using Nei’s G_st indicate that about 50·60 per cent of the observed variability is due to interdemic subdivision. In accordance, populations within a species show a much lower level of variability, however some populations harbour individuals that are very divergent from the rest of their conspeciiics at the same locality. We review two possible mechanisms that could explain the presence of these divergent individuals, hybridisation and the effects of stochastic branching processes.

Original language	English
Pages (from-to)	75-85
Number of pages	11
Journal	Heredity
Volume	56
Issue number	1
DOIs	https://doi.org/10.1038/hdy.1986.11
State	Published - Feb 1986
Externally published	Yes

Bibliographical note

Funding Information:
liams and K. Y. Kaneshiro for assistance in collecting the flies used in this study; and Linden Teromoto and K. Y. Kaneshiro for help in rearing the flies in the lab. We also thank K. Y. Kaneshiro, H. L. Carson, T. Crease, J. Learn, E. A. Zimmer, S. M. Williams and R. Zink for comments on earlier draftE We would also like to thank an anonymous reviewer for extensive comments and criticism. This work was supported by NIH Grant ROl GM31571 to Alan Temoleton.

ASJC Scopus subject areas

Genetics
Genetics(clinical)

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10.1038/hdy.1986.11

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title = "Mitochondrial dna variability in natural populations of hawaiian drosophila. I. methods and levels of variability in d. silvestris and d. heteroneura populations",

abstract = "We describe techniques by which mitochondrial DNA (mtDNA) restriction site information can be obtained for up to 16 different restriction endonucleases on individual Hawaiian Drosophila, in particular D. silvestris and D. heteroneura. We have constructed mtDNA restriction site maps for a total of forty-eight wild caught genomes of both species, from seven major collecting sites on the island of Hawaii. Levels of variability are, in general, high in D. silvestris (p of Ewens et al., 1981 =0·0486) and lower in D. heteroneura (p =0·0327). Measures of population subdivision using Nei{\textquoteright}s Gst indicate that about 50·60 per cent of the observed variability is due to interdemic subdivision. In accordance, populations within a species show a much lower level of variability, however some populations harbour individuals that are very divergent from the rest of their conspeciiics at the same locality. We review two possible mechanisms that could explain the presence of these divergent individuals, hybridisation and the effects of stochastic branching processes.",

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note = "Funding Information: liams and K. Y. Kaneshiro for assistance in collecting the flies used in this study; and Linden Teromoto and K. Y. Kaneshiro for help in rearing the flies in the lab. We also thank K. Y. Kaneshiro, H. L. Carson, T. Crease, J. Learn, E. A. Zimmer, S. M. Williams and R. Zink for comments on earlier draftE We would also like to thank an anonymous reviewer for extensive comments and criticism. This work was supported by NIH Grant ROl GM31571 to Alan Temoleton.",

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N1 - Funding Information: liams and K. Y. Kaneshiro for assistance in collecting the flies used in this study; and Linden Teromoto and K. Y. Kaneshiro for help in rearing the flies in the lab. We also thank K. Y. Kaneshiro, H. L. Carson, T. Crease, J. Learn, E. A. Zimmer, S. M. Williams and R. Zink for comments on earlier draftE We would also like to thank an anonymous reviewer for extensive comments and criticism. This work was supported by NIH Grant ROl GM31571 to Alan Temoleton.

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N2 - We describe techniques by which mitochondrial DNA (mtDNA) restriction site information can be obtained for up to 16 different restriction endonucleases on individual Hawaiian Drosophila, in particular D. silvestris and D. heteroneura. We have constructed mtDNA restriction site maps for a total of forty-eight wild caught genomes of both species, from seven major collecting sites on the island of Hawaii. Levels of variability are, in general, high in D. silvestris (p of Ewens et al., 1981 =0·0486) and lower in D. heteroneura (p =0·0327). Measures of population subdivision using Nei’s Gst indicate that about 50·60 per cent of the observed variability is due to interdemic subdivision. In accordance, populations within a species show a much lower level of variability, however some populations harbour individuals that are very divergent from the rest of their conspeciiics at the same locality. We review two possible mechanisms that could explain the presence of these divergent individuals, hybridisation and the effects of stochastic branching processes.

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Mitochondrial dna variability in natural populations of hawaiian drosophila. I. methods and levels of variability in d. silvestris and d. heteroneura populations

Abstract

Bibliographical note

ASJC Scopus subject areas

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