Microscale ecological stress causes RAPD molecular selection in wild barley, Neve Yaar microsite, Israel

E. D. Owuor; A. Beharav; T. Fahima; V. M. Kirzhner; A. B. Korol; E. Nevo

doi:10.1023/A:1022995214885

Microscale ecological stress causes RAPD molecular selection in wild barley, Neve Yaar microsite, Israel

E. D. Owuor, A. Beharav, T. Fahima, V. M. Kirzhner, A. B. Korol, E. Nevo

Department of Evolutionary and Environmental Biology

Research output: Contribution to journal › Article › peer-review

Abstract

Genetic micro-differentiation was studied in wild barley at the Neve Yaar microsite, Israel, an area of 3182 m², which includes a mosaic of sun-rock, sun-soil, shade-rock, and shade-soil microniches. Fifty-six genotypes were tested for ecological-genetic microniche correlates. Analysis of 75 RAPD loci revealed a significantly higher polymorphism and gene diversity (P_0.05 = 0.920; He = 0.411) on the more stressful sun-rock microniche, compared to the least stressful shade-soil microniche (P_0.05 = 0.653; He = 0.188). Fifty-six (74.7%) out of the 75 loci varied significantly in allele frequencies. On the average, of the 75 loci, 75.7% of the total genetic diversity exists within the four microniches, while 24.3% exists between the microniches. The highest genetic distance was between shade-soil and sun-rock (D = 0.220), which confirmed their sharp microecological contrasts. Gametic phase disequilibria (LD) differed significantly between sunny and shady microniches. The levels of correct classification of individual genotypes into their respective microniche, based on the presence/absence of bands of the 2, 3, 4, 5, and 7 best differentiating loci, obtained by stepwise discriminant analysis, were 79, 84, 89, 95, and 100%, respectively. We conclude that ecological contrasts at the Neve Yaar microsite cause molecular diversifying selection (presumably at both coding and noncoding genomic regions of wild barley) overriding gene flow.

Original language	English
Pages (from-to)	213-224
Number of pages	12
Journal	Genetic Resources and Crop Evolution
Volume	50
Issue number	2
DOIs	https://doi.org/10.1023/A:1022995214885
State	Published - Mar 2003

Bibliographical note

Funding Information:
Edward D. Owuor, supported by the Authority for Advanced Studies at the University of Haifa, the Israel Discount Bank Chair of Evolutionary Biology, and the Ancell-Teicher Research Foundation for Genetics and Molecular Evolution. The authors thank Avigdor Beiles and Eric Hallerman for critically reading the manuscript.

Keywords

Hordeum spontaneum
Microscale selection
RAPD

ASJC Scopus subject areas

Ecology, Evolution, Behavior and Systematics
Agronomy and Crop Science
Genetics
Plant Science

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1023/A:1022995214885

Cite this

@article{19277085c6ed46a8b095b85f2b3490d2,

title = "Microscale ecological stress causes RAPD molecular selection in wild barley, Neve Yaar microsite, Israel",

abstract = "Genetic micro-differentiation was studied in wild barley at the Neve Yaar microsite, Israel, an area of 3182 m2, which includes a mosaic of sun-rock, sun-soil, shade-rock, and shade-soil microniches. Fifty-six genotypes were tested for ecological-genetic microniche correlates. Analysis of 75 RAPD loci revealed a significantly higher polymorphism and gene diversity (P0.05 = 0.920; He = 0.411) on the more stressful sun-rock microniche, compared to the least stressful shade-soil microniche (P0.05 = 0.653; He = 0.188). Fifty-six (74.7%) out of the 75 loci varied significantly in allele frequencies. On the average, of the 75 loci, 75.7% of the total genetic diversity exists within the four microniches, while 24.3% exists between the microniches. The highest genetic distance was between shade-soil and sun-rock (D = 0.220), which confirmed their sharp microecological contrasts. Gametic phase disequilibria (LD) differed significantly between sunny and shady microniches. The levels of correct classification of individual genotypes into their respective microniche, based on the presence/absence of bands of the 2, 3, 4, 5, and 7 best differentiating loci, obtained by stepwise discriminant analysis, were 79, 84, 89, 95, and 100%, respectively. We conclude that ecological contrasts at the Neve Yaar microsite cause molecular diversifying selection (presumably at both coding and noncoding genomic regions of wild barley) overriding gene flow.",

keywords = "Hordeum spontaneum, Microscale selection, RAPD",

author = "Owuor, {E. D.} and A. Beharav and T. Fahima and Kirzhner, {V. M.} and Korol, {A. B.} and E. Nevo",

note = "Funding Information: Edward D. Owuor, supported by the Authority for Advanced Studies at the University of Haifa, the Israel Discount Bank Chair of Evolutionary Biology, and the Ancell-Teicher Research Foundation for Genetics and Molecular Evolution. The authors thank Avigdor Beiles and Eric Hallerman for critically reading the manuscript.",

year = "2003",

month = mar,

doi = "10.1023/A:1022995214885",

language = "English",

volume = "50",

pages = "213--224",

journal = "Genetic Resources and Crop Evolution",

issn = "0925-9864",

publisher = "Springer Netherlands",

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TY - JOUR

T1 - Microscale ecological stress causes RAPD molecular selection in wild barley, Neve Yaar microsite, Israel

AU - Owuor, E. D.

AU - Beharav, A.

AU - Fahima, T.

AU - Kirzhner, V. M.

AU - Korol, A. B.

AU - Nevo, E.

N1 - Funding Information: Edward D. Owuor, supported by the Authority for Advanced Studies at the University of Haifa, the Israel Discount Bank Chair of Evolutionary Biology, and the Ancell-Teicher Research Foundation for Genetics and Molecular Evolution. The authors thank Avigdor Beiles and Eric Hallerman for critically reading the manuscript.

PY - 2003/3

Y1 - 2003/3

N2 - Genetic micro-differentiation was studied in wild barley at the Neve Yaar microsite, Israel, an area of 3182 m2, which includes a mosaic of sun-rock, sun-soil, shade-rock, and shade-soil microniches. Fifty-six genotypes were tested for ecological-genetic microniche correlates. Analysis of 75 RAPD loci revealed a significantly higher polymorphism and gene diversity (P0.05 = 0.920; He = 0.411) on the more stressful sun-rock microniche, compared to the least stressful shade-soil microniche (P0.05 = 0.653; He = 0.188). Fifty-six (74.7%) out of the 75 loci varied significantly in allele frequencies. On the average, of the 75 loci, 75.7% of the total genetic diversity exists within the four microniches, while 24.3% exists between the microniches. The highest genetic distance was between shade-soil and sun-rock (D = 0.220), which confirmed their sharp microecological contrasts. Gametic phase disequilibria (LD) differed significantly between sunny and shady microniches. The levels of correct classification of individual genotypes into their respective microniche, based on the presence/absence of bands of the 2, 3, 4, 5, and 7 best differentiating loci, obtained by stepwise discriminant analysis, were 79, 84, 89, 95, and 100%, respectively. We conclude that ecological contrasts at the Neve Yaar microsite cause molecular diversifying selection (presumably at both coding and noncoding genomic regions of wild barley) overriding gene flow.

AB - Genetic micro-differentiation was studied in wild barley at the Neve Yaar microsite, Israel, an area of 3182 m2, which includes a mosaic of sun-rock, sun-soil, shade-rock, and shade-soil microniches. Fifty-six genotypes were tested for ecological-genetic microniche correlates. Analysis of 75 RAPD loci revealed a significantly higher polymorphism and gene diversity (P0.05 = 0.920; He = 0.411) on the more stressful sun-rock microniche, compared to the least stressful shade-soil microniche (P0.05 = 0.653; He = 0.188). Fifty-six (74.7%) out of the 75 loci varied significantly in allele frequencies. On the average, of the 75 loci, 75.7% of the total genetic diversity exists within the four microniches, while 24.3% exists between the microniches. The highest genetic distance was between shade-soil and sun-rock (D = 0.220), which confirmed their sharp microecological contrasts. Gametic phase disequilibria (LD) differed significantly between sunny and shady microniches. The levels of correct classification of individual genotypes into their respective microniche, based on the presence/absence of bands of the 2, 3, 4, 5, and 7 best differentiating loci, obtained by stepwise discriminant analysis, were 79, 84, 89, 95, and 100%, respectively. We conclude that ecological contrasts at the Neve Yaar microsite cause molecular diversifying selection (presumably at both coding and noncoding genomic regions of wild barley) overriding gene flow.

KW - Hordeum spontaneum

KW - Microscale selection

KW - RAPD

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DO - 10.1023/A:1022995214885

M3 - Article

AN - SCOPUS:0037679204

SN - 0925-9864

VL - 50

SP - 213

EP - 224

JO - Genetic Resources and Crop Evolution

JF - Genetic Resources and Crop Evolution

IS - 2

ER -

Microscale ecological stress causes RAPD molecular selection in wild barley, Neve Yaar microsite, Israel

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

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