microRNAs selectively protect hub cells of the germline stem cell niche from apoptosis

Marina Volin; Maayan Zohar-Fux; Oren Gonen; Lilach Porat-Kuperstein; Hila Toledano

doi:10.1083/jcb.201711098

microRNAs selectively protect hub cells of the germline stem cell niche from apoptosis

Marina Volin, Maayan Zohar-Fux, Oren Gonen, Lilach Porat-Kuperstein, Hila Toledano

Research output: Contribution to journal › Article › peer-review

Abstract

Genotoxic stress such as irradiation causes a temporary halt in tissue regeneration. The ability to regain regeneration depends on the type of cells that survived the assault. Previous studies showed that this propensity is usually held by the tissue-specific stem cells. However, stem cells cannot maintain their unique properties without the support of their surrounding niche cells. In this study, we show that exposure of Drosophila melanogaster to extremely high levels of irradiation temporarily arrests spermatogenesis and kills half of the stem cells. In marked contrast, the hub cells that constitute a major component of the niche remain completely intact. We further show that this atypical resistance to cell death relies on the expression of certain antiapoptotic microRNAs (miRNAs) that are selectively expressed in the hub and keep the cells inert to apoptotic stress signals. We propose that at the tissue level, protection of a specific group of niche cells from apoptosis underlies ongoing stem cell turnover and tissue regeneration.

Original language	English
Pages (from-to)	3829-3838
Number of pages	10
Journal	Journal of Cell Biology
Volume	217
Issue number	11
DOIs	https://doi.org/10.1083/jcb.201711098
State	Published - 1 Nov 2018

Bibliographical note

Publisher Copyright:
© 2018 Volin et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

ASJC Scopus subject areas

Cell Biology

Access to Document

10.1083/jcb.201711098

Cite this

@article{b51d7dcbf520499092000a5ff9c9f062,

title = "microRNAs selectively protect hub cells of the germline stem cell niche from apoptosis",

abstract = "Genotoxic stress such as irradiation causes a temporary halt in tissue regeneration. The ability to regain regeneration depends on the type of cells that survived the assault. Previous studies showed that this propensity is usually held by the tissue-specific stem cells. However, stem cells cannot maintain their unique properties without the support of their surrounding niche cells. In this study, we show that exposure of Drosophila melanogaster to extremely high levels of irradiation temporarily arrests spermatogenesis and kills half of the stem cells. In marked contrast, the hub cells that constitute a major component of the niche remain completely intact. We further show that this atypical resistance to cell death relies on the expression of certain antiapoptotic microRNAs (miRNAs) that are selectively expressed in the hub and keep the cells inert to apoptotic stress signals. We propose that at the tissue level, protection of a specific group of niche cells from apoptosis underlies ongoing stem cell turnover and tissue regeneration.",

author = "Marina Volin and Maayan Zohar-Fux and Oren Gonen and Lilach Porat-Kuperstein and Hila Toledano",

note = "Publisher Copyright: {\textcopyright} 2018 Volin et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).",

year = "2018",

month = nov,

day = "1",

doi = "10.1083/jcb.201711098",

language = "English",

volume = "217",

pages = "3829--3838",

journal = "Journal of Cell Biology",

issn = "0021-9525",

publisher = "Rockefeller University Press",

number = "11",

}

TY - JOUR

T1 - microRNAs selectively protect hub cells of the germline stem cell niche from apoptosis

AU - Volin, Marina

AU - Zohar-Fux, Maayan

AU - Gonen, Oren

AU - Porat-Kuperstein, Lilach

AU - Toledano, Hila

N1 - Publisher Copyright: © 2018 Volin et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PY - 2018/11/1

Y1 - 2018/11/1

N2 - Genotoxic stress such as irradiation causes a temporary halt in tissue regeneration. The ability to regain regeneration depends on the type of cells that survived the assault. Previous studies showed that this propensity is usually held by the tissue-specific stem cells. However, stem cells cannot maintain their unique properties without the support of their surrounding niche cells. In this study, we show that exposure of Drosophila melanogaster to extremely high levels of irradiation temporarily arrests spermatogenesis and kills half of the stem cells. In marked contrast, the hub cells that constitute a major component of the niche remain completely intact. We further show that this atypical resistance to cell death relies on the expression of certain antiapoptotic microRNAs (miRNAs) that are selectively expressed in the hub and keep the cells inert to apoptotic stress signals. We propose that at the tissue level, protection of a specific group of niche cells from apoptosis underlies ongoing stem cell turnover and tissue regeneration.

AB - Genotoxic stress such as irradiation causes a temporary halt in tissue regeneration. The ability to regain regeneration depends on the type of cells that survived the assault. Previous studies showed that this propensity is usually held by the tissue-specific stem cells. However, stem cells cannot maintain their unique properties without the support of their surrounding niche cells. In this study, we show that exposure of Drosophila melanogaster to extremely high levels of irradiation temporarily arrests spermatogenesis and kills half of the stem cells. In marked contrast, the hub cells that constitute a major component of the niche remain completely intact. We further show that this atypical resistance to cell death relies on the expression of certain antiapoptotic microRNAs (miRNAs) that are selectively expressed in the hub and keep the cells inert to apoptotic stress signals. We propose that at the tissue level, protection of a specific group of niche cells from apoptosis underlies ongoing stem cell turnover and tissue regeneration.

UR - http://www.scopus.com/inward/record.url?scp=85056259049&partnerID=8YFLogxK

U2 - 10.1083/jcb.201711098

DO - 10.1083/jcb.201711098

M3 - Article

C2 - 30093492

AN - SCOPUS:85056259049

SN - 0021-9525

VL - 217

SP - 3829

EP - 3838

JO - Journal of Cell Biology

JF - Journal of Cell Biology

IS - 11

ER -

microRNAs selectively protect hub cells of the germline stem cell niche from apoptosis

Abstract

Bibliographical note

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this