Metallothionein induction in the RTH-149 cell line as an indicator for heavy metal bioavailability in a brackish environment: Assessment by RT-competitive PCR

I. Kamer; J. Douek; M. Tom; B. Rinkevich

doi:10.1007/s00244-002-0214-7

Metallothionein induction in the RTH-149 cell line as an indicator for heavy metal bioavailability in a brackish environment: Assessment by RT-competitive PCR

I. Kamer, J. Douek, M. Tom, B. Rinkevich

Research output: Contribution to journal › Article › peer-review

Abstract

Estuarine and marine near-shore environments are often subjected to heavy metal pollution. We establish a bioassay using the quantitative evaluation of metallothionein (MT) transcript in the fish hepatoma cell line, RTH-149, as a tool for detecting heavy metal pollution in brackish-marine water containing other pollutants in addition to heavy metals. RT-competitive polymerase chain reaction was used for the quantitative evaluation of the transcript in absolute units. Cadmium was used as a model pollutant to optimize two parameters of the assay: exposure periods (24, 96, 144 h) and salinity (0%, 25%, 50%, 75%, 100% sea water). Results revealed that salinity at or below 25% sea water at an exposure period of 144 h are the preferable conditions for detecting MT mRNA levels for in vitro assays employed on water samples from highly polluted brackish habitats.

Original language	English
Pages (from-to)	86-91
Number of pages	6
Journal	Archives of Environmental Contamination and Toxicology
Volume	45
Issue number	1
DOIs	https://doi.org/10.1007/s00244-002-0214-7
State	Published - Jul 2003
Externally published	Yes

ASJC Scopus subject areas

Toxicology
Pollution
Health, Toxicology and Mutagenesis

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/s00244-002-0214-7

Cite this

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abstract = "Estuarine and marine near-shore environments are often subjected to heavy metal pollution. We establish a bioassay using the quantitative evaluation of metallothionein (MT) transcript in the fish hepatoma cell line, RTH-149, as a tool for detecting heavy metal pollution in brackish-marine water containing other pollutants in addition to heavy metals. RT-competitive polymerase chain reaction was used for the quantitative evaluation of the transcript in absolute units. Cadmium was used as a model pollutant to optimize two parameters of the assay: exposure periods (24, 96, 144 h) and salinity (0\%, 25\%, 50\%, 75\%, 100\% sea water). Results revealed that salinity at or below 25\% sea water at an exposure period of 144 h are the preferable conditions for detecting MT mRNA levels for in vitro assays employed on water samples from highly polluted brackish habitats.",

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AU - Kamer, I.

AU - Douek, J.

AU - Tom, M.

AU - Rinkevich, B.

PY - 2003/7

Y1 - 2003/7

N2 - Estuarine and marine near-shore environments are often subjected to heavy metal pollution. We establish a bioassay using the quantitative evaluation of metallothionein (MT) transcript in the fish hepatoma cell line, RTH-149, as a tool for detecting heavy metal pollution in brackish-marine water containing other pollutants in addition to heavy metals. RT-competitive polymerase chain reaction was used for the quantitative evaluation of the transcript in absolute units. Cadmium was used as a model pollutant to optimize two parameters of the assay: exposure periods (24, 96, 144 h) and salinity (0%, 25%, 50%, 75%, 100% sea water). Results revealed that salinity at or below 25% sea water at an exposure period of 144 h are the preferable conditions for detecting MT mRNA levels for in vitro assays employed on water samples from highly polluted brackish habitats.

AB - Estuarine and marine near-shore environments are often subjected to heavy metal pollution. We establish a bioassay using the quantitative evaluation of metallothionein (MT) transcript in the fish hepatoma cell line, RTH-149, as a tool for detecting heavy metal pollution in brackish-marine water containing other pollutants in addition to heavy metals. RT-competitive polymerase chain reaction was used for the quantitative evaluation of the transcript in absolute units. Cadmium was used as a model pollutant to optimize two parameters of the assay: exposure periods (24, 96, 144 h) and salinity (0%, 25%, 50%, 75%, 100% sea water). Results revealed that salinity at or below 25% sea water at an exposure period of 144 h are the preferable conditions for detecting MT mRNA levels for in vitro assays employed on water samples from highly polluted brackish habitats.

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Metallothionein induction in the RTH-149 cell line as an indicator for heavy metal bioavailability in a brackish environment: Assessment by RT-competitive PCR

Abstract

ASJC Scopus subject areas

UN SDGs

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