TY - JOUR
T1 - Metallothionein induction in the RTH-149 cell line as an indicator for heavy metal bioavailability in a brackish environment
T2 - Assessment by RT-competitive PCR
AU - Kamer, I.
AU - Douek, J.
AU - Tom, M.
AU - Rinkevich, B.
PY - 2003/7
Y1 - 2003/7
N2 - Estuarine and marine near-shore environments are often subjected to heavy metal pollution. We establish a bioassay using the quantitative evaluation of metallothionein (MT) transcript in the fish hepatoma cell line, RTH-149, as a tool for detecting heavy metal pollution in brackish-marine water containing other pollutants in addition to heavy metals. RT-competitive polymerase chain reaction was used for the quantitative evaluation of the transcript in absolute units. Cadmium was used as a model pollutant to optimize two parameters of the assay: exposure periods (24, 96, 144 h) and salinity (0%, 25%, 50%, 75%, 100% sea water). Results revealed that salinity at or below 25% sea water at an exposure period of 144 h are the preferable conditions for detecting MT mRNA levels for in vitro assays employed on water samples from highly polluted brackish habitats.
AB - Estuarine and marine near-shore environments are often subjected to heavy metal pollution. We establish a bioassay using the quantitative evaluation of metallothionein (MT) transcript in the fish hepatoma cell line, RTH-149, as a tool for detecting heavy metal pollution in brackish-marine water containing other pollutants in addition to heavy metals. RT-competitive polymerase chain reaction was used for the quantitative evaluation of the transcript in absolute units. Cadmium was used as a model pollutant to optimize two parameters of the assay: exposure periods (24, 96, 144 h) and salinity (0%, 25%, 50%, 75%, 100% sea water). Results revealed that salinity at or below 25% sea water at an exposure period of 144 h are the preferable conditions for detecting MT mRNA levels for in vitro assays employed on water samples from highly polluted brackish habitats.
UR - http://www.scopus.com/inward/record.url?scp=0038141157&partnerID=8YFLogxK
U2 - 10.1007/s00244-002-0214-7
DO - 10.1007/s00244-002-0214-7
M3 - Article
C2 - 12948177
AN - SCOPUS:0038141157
SN - 0090-4341
VL - 45
SP - 86
EP - 91
JO - Archives of Environmental Contamination and Toxicology
JF - Archives of Environmental Contamination and Toxicology
IS - 1
ER -
