Mechanisms Underlying the Hyperexcitability of CA3 and Dentate Gyrus Hippocampal Neurons Derived From Patients With Bipolar Disorder

Shani Stern; Anindita Sarkar; Tchelet Stern; Arianna Mei; Ana P.D. Mendes; Yam Stern; Gabriela Goldberg; Dekel Galor; Thao Nguyen; Lynne Randolph-Moore; Yongsung Kim; Guy Rouleau; Anne Bang; Martin Alda; Renata Santos; Maria C. Marchetto; Fred H. Gage

doi:10.1016/j.biopsych.2019.09.018

Mechanisms Underlying the Hyperexcitability of CA3 and Dentate Gyrus Hippocampal Neurons Derived From Patients With Bipolar Disorder

Shani Stern, Anindita Sarkar, Tchelet Stern, Arianna Mei, Ana P.D. Mendes, Yam Stern, Gabriela Goldberg, Dekel Galor, Thao Nguyen, Lynne Randolph-Moore, Yongsung Kim, Guy Rouleau, Anne Bang, Martin Alda, Renata Santos, Maria C. Marchetto, Fred H. Gage

Department of Neurobiology

Research output: Contribution to journal › Article › peer-review

Abstract

Background: Approximately 1 in every 50 to 100 people is affected with bipolar disorder (BD), making this disease a major economic burden. The introduction of induced pluripotent stem cell methodology enabled better modeling of this disorder. Methods: Having previously studied the phenotype of dentate gyrus granule neurons, we turned our attention to studying the phenotype of CA3 hippocampal pyramidal neurons of 6 patients with BD compared with 4 control individuals. We used patch clamp and quantitative polymerase chain reaction to measure electrophysiological features and RNA expression by specific channel genes. Results: We found that BD CA3 neurons were hyperexcitable only when they were derived from patients who responded to lithium; they featured sustained activity with large current injections and a large, fast after-hyperpolarization, similar to what we previously reported in dentate gyrus neurons. The higher amplitudes and faster kinetics of fast potassium currents correlated with this hyperexcitability. Further supporting the involvement of potassium currents, we observed an overexpression of KCNC1 and KCNC2 in hippocampal neurons derived from lithium responders. Applying specific potassium channel blockers diminished the hyperexcitability. Long-term lithium treatment decreased the hyperexcitability observed in the CA3 neurons derived from lithium responders while increasing sodium currents and reducing fast potassium currents. When differentiating this cohort into spinal motor neurons, we did not observe any changes in the excitability of BD motor neurons compared with control motor neurons. Conclusions: The hyperexcitability of BD neurons is neuronal type specific with the involvement of altered potassium currents that allow for a sustained, continued firing activity.

Original language	English
Pages (from-to)	139-149
Number of pages	11
Journal	Biological Psychiatry
Volume	88
Issue number	2
DOIs	https://doi.org/10.1016/j.biopsych.2019.09.018
State	Published - 15 Jul 2020

Bibliographical note

Funding Information:
This work was supported in part by the National Cancer Institute (Grant No. P30 CA014195) and the National Institutes of Health (Grant No. R01 AG05651 [to FG]) and by the National Cooperative Reprogrammed Cell Research Groups (NCRCRG) (Grant No. U19 MH106434 [to FG]). The Gage laboratory is supported in part by the Leona M. and Harry B. Helmsley Charitable Trust Grant No. 2017-PG-MED001, the JPB Foundation, Annette C. Merle-Smith, and the Robert and Mary Jane Engman Foundation. The lymphoblast samples and the clinical data were obtained with support from Canadian Institutes of Health Research Grant No. 64410 (to MA and GR). We thank Mary Lynn Gage for editing the article. We thank the Salk core facilities, and in addition, we thank the Engamm Foundation, JPB Foundation, and Streim Foundation. The authors report no biomedical financial interests or potential conflicts of interest.

Funding Information:
This work was supported in part by the National Cancer Institute (Grant No. P30 CA014195 ) and the National Institutes of Health (Grant No. R01 AG05651 [to FG]) and by the National Cooperative Reprogrammed Cell Research Groups (NCRCRG) (Grant No. U19 MH106434 [to FG]). The Gage laboratory is supported in part by the Leona M. and Harry B. Helmsley Charitable Trust Grant No. 2017-PG-MED001 , the JPB Foundation, Annette C. Merle-Smith, and the Robert and Mary Jane Engman Foundation. The lymphoblast samples and the clinical data were obtained with support from Canadian Institutes of Health Research Grant No. 64410 (to MA and GR). We thank Mary Lynn Gage for editing the article. We thank the Salk core facilities, and in addition, we thank the Engamm Foundation, JPB Foundation, and Streim Foundation. The authors report no biomedical financial interests or potential conflicts of interest.

Publisher Copyright:
© 2019 Society of Biological Psychiatry

Keywords

Bipolar disorder
Dentate gyrus
Hippocampus
Hyperexcitability
Motor neurons
Pyramidal

ASJC Scopus subject areas

Biological Psychiatry

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10.1016/j.biopsych.2019.09.018

Cite this

Stern, S., Sarkar, A., Stern, T., Mei, A., Mendes, A. P. D., Stern, Y., Goldberg, G., Galor, D., Nguyen, T., Randolph-Moore, L., Kim, Y., Rouleau, G., Bang, A., Alda, M., Santos, R., Marchetto, M. C., & Gage, F. H. (2020). Mechanisms Underlying the Hyperexcitability of CA3 and Dentate Gyrus Hippocampal Neurons Derived From Patients With Bipolar Disorder. Biological Psychiatry, 88(2), 139-149. https://doi.org/10.1016/j.biopsych.2019.09.018

@article{f813833d01a2451aa5446c9158dab820,

title = "Mechanisms Underlying the Hyperexcitability of CA3 and Dentate Gyrus Hippocampal Neurons Derived From Patients With Bipolar Disorder",

abstract = "Background: Approximately 1 in every 50 to 100 people is affected with bipolar disorder (BD), making this disease a major economic burden. The introduction of induced pluripotent stem cell methodology enabled better modeling of this disorder. Methods: Having previously studied the phenotype of dentate gyrus granule neurons, we turned our attention to studying the phenotype of CA3 hippocampal pyramidal neurons of 6 patients with BD compared with 4 control individuals. We used patch clamp and quantitative polymerase chain reaction to measure electrophysiological features and RNA expression by specific channel genes. Results: We found that BD CA3 neurons were hyperexcitable only when they were derived from patients who responded to lithium; they featured sustained activity with large current injections and a large, fast after-hyperpolarization, similar to what we previously reported in dentate gyrus neurons. The higher amplitudes and faster kinetics of fast potassium currents correlated with this hyperexcitability. Further supporting the involvement of potassium currents, we observed an overexpression of KCNC1 and KCNC2 in hippocampal neurons derived from lithium responders. Applying specific potassium channel blockers diminished the hyperexcitability. Long-term lithium treatment decreased the hyperexcitability observed in the CA3 neurons derived from lithium responders while increasing sodium currents and reducing fast potassium currents. When differentiating this cohort into spinal motor neurons, we did not observe any changes in the excitability of BD motor neurons compared with control motor neurons. Conclusions: The hyperexcitability of BD neurons is neuronal type specific with the involvement of altered potassium currents that allow for a sustained, continued firing activity.",

keywords = "Bipolar disorder, Dentate gyrus, Hippocampus, Hyperexcitability, Motor neurons, Pyramidal",

author = "Shani Stern and Anindita Sarkar and Tchelet Stern and Arianna Mei and Mendes, {Ana P.D.} and Yam Stern and Gabriela Goldberg and Dekel Galor and Thao Nguyen and Lynne Randolph-Moore and Yongsung Kim and Guy Rouleau and Anne Bang and Martin Alda and Renata Santos and Marchetto, {Maria C.} and Gage, {Fred H.}",

note = "Funding Information: This work was supported in part by the National Cancer Institute (Grant No. P30 CA014195) and the National Institutes of Health (Grant No. R01 AG05651 [to FG]) and by the National Cooperative Reprogrammed Cell Research Groups (NCRCRG) (Grant No. U19 MH106434 [to FG]). The Gage laboratory is supported in part by the Leona M. and Harry B. Helmsley Charitable Trust Grant No. 2017-PG-MED001, the JPB Foundation, Annette C. Merle-Smith, and the Robert and Mary Jane Engman Foundation. The lymphoblast samples and the clinical data were obtained with support from Canadian Institutes of Health Research Grant No. 64410 (to MA and GR). We thank Mary Lynn Gage for editing the article. We thank the Salk core facilities, and in addition, we thank the Engamm Foundation, JPB Foundation, and Streim Foundation. The authors report no biomedical financial interests or potential conflicts of interest. Funding Information: This work was supported in part by the National Cancer Institute (Grant No. P30 CA014195 ) and the National Institutes of Health (Grant No. R01 AG05651 [to FG]) and by the National Cooperative Reprogrammed Cell Research Groups (NCRCRG) (Grant No. U19 MH106434 [to FG]). The Gage laboratory is supported in part by the Leona M. and Harry B. Helmsley Charitable Trust Grant No. 2017-PG-MED001 , the JPB Foundation, Annette C. Merle-Smith, and the Robert and Mary Jane Engman Foundation. The lymphoblast samples and the clinical data were obtained with support from Canadian Institutes of Health Research Grant No. 64410 (to MA and GR). We thank Mary Lynn Gage for editing the article. We thank the Salk core facilities, and in addition, we thank the Engamm Foundation, JPB Foundation, and Streim Foundation. The authors report no biomedical financial interests or potential conflicts of interest. Publisher Copyright: {\textcopyright} 2019 Society of Biological Psychiatry",

year = "2020",

month = jul,

day = "15",

doi = "10.1016/j.biopsych.2019.09.018",

language = "English",

volume = "88",

pages = "139--149",

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Stern, S, Sarkar, A, Stern, T, Mei, A, Mendes, APD, Stern, Y, Goldberg, G, Galor, D, Nguyen, T, Randolph-Moore, L, Kim, Y, Rouleau, G, Bang, A, Alda, M, Santos, R, Marchetto, MC & Gage, FH 2020, 'Mechanisms Underlying the Hyperexcitability of CA3 and Dentate Gyrus Hippocampal Neurons Derived From Patients With Bipolar Disorder', Biological Psychiatry, vol. 88, no. 2, pp. 139-149. https://doi.org/10.1016/j.biopsych.2019.09.018

TY - JOUR

T1 - Mechanisms Underlying the Hyperexcitability of CA3 and Dentate Gyrus Hippocampal Neurons Derived From Patients With Bipolar Disorder

AU - Stern, Shani

AU - Sarkar, Anindita

AU - Stern, Tchelet

AU - Mei, Arianna

AU - Mendes, Ana P.D.

AU - Stern, Yam

AU - Goldberg, Gabriela

AU - Galor, Dekel

AU - Nguyen, Thao

AU - Randolph-Moore, Lynne

AU - Kim, Yongsung

AU - Rouleau, Guy

AU - Bang, Anne

AU - Alda, Martin

AU - Santos, Renata

AU - Marchetto, Maria C.

AU - Gage, Fred H.

N1 - Funding Information: This work was supported in part by the National Cancer Institute (Grant No. P30 CA014195) and the National Institutes of Health (Grant No. R01 AG05651 [to FG]) and by the National Cooperative Reprogrammed Cell Research Groups (NCRCRG) (Grant No. U19 MH106434 [to FG]). The Gage laboratory is supported in part by the Leona M. and Harry B. Helmsley Charitable Trust Grant No. 2017-PG-MED001, the JPB Foundation, Annette C. Merle-Smith, and the Robert and Mary Jane Engman Foundation. The lymphoblast samples and the clinical data were obtained with support from Canadian Institutes of Health Research Grant No. 64410 (to MA and GR). We thank Mary Lynn Gage for editing the article. We thank the Salk core facilities, and in addition, we thank the Engamm Foundation, JPB Foundation, and Streim Foundation. The authors report no biomedical financial interests or potential conflicts of interest. Funding Information: This work was supported in part by the National Cancer Institute (Grant No. P30 CA014195 ) and the National Institutes of Health (Grant No. R01 AG05651 [to FG]) and by the National Cooperative Reprogrammed Cell Research Groups (NCRCRG) (Grant No. U19 MH106434 [to FG]). The Gage laboratory is supported in part by the Leona M. and Harry B. Helmsley Charitable Trust Grant No. 2017-PG-MED001 , the JPB Foundation, Annette C. Merle-Smith, and the Robert and Mary Jane Engman Foundation. The lymphoblast samples and the clinical data were obtained with support from Canadian Institutes of Health Research Grant No. 64410 (to MA and GR). We thank Mary Lynn Gage for editing the article. We thank the Salk core facilities, and in addition, we thank the Engamm Foundation, JPB Foundation, and Streim Foundation. The authors report no biomedical financial interests or potential conflicts of interest. Publisher Copyright: © 2019 Society of Biological Psychiatry

PY - 2020/7/15

Y1 - 2020/7/15

N2 - Background: Approximately 1 in every 50 to 100 people is affected with bipolar disorder (BD), making this disease a major economic burden. The introduction of induced pluripotent stem cell methodology enabled better modeling of this disorder. Methods: Having previously studied the phenotype of dentate gyrus granule neurons, we turned our attention to studying the phenotype of CA3 hippocampal pyramidal neurons of 6 patients with BD compared with 4 control individuals. We used patch clamp and quantitative polymerase chain reaction to measure electrophysiological features and RNA expression by specific channel genes. Results: We found that BD CA3 neurons were hyperexcitable only when they were derived from patients who responded to lithium; they featured sustained activity with large current injections and a large, fast after-hyperpolarization, similar to what we previously reported in dentate gyrus neurons. The higher amplitudes and faster kinetics of fast potassium currents correlated with this hyperexcitability. Further supporting the involvement of potassium currents, we observed an overexpression of KCNC1 and KCNC2 in hippocampal neurons derived from lithium responders. Applying specific potassium channel blockers diminished the hyperexcitability. Long-term lithium treatment decreased the hyperexcitability observed in the CA3 neurons derived from lithium responders while increasing sodium currents and reducing fast potassium currents. When differentiating this cohort into spinal motor neurons, we did not observe any changes in the excitability of BD motor neurons compared with control motor neurons. Conclusions: The hyperexcitability of BD neurons is neuronal type specific with the involvement of altered potassium currents that allow for a sustained, continued firing activity.

AB - Background: Approximately 1 in every 50 to 100 people is affected with bipolar disorder (BD), making this disease a major economic burden. The introduction of induced pluripotent stem cell methodology enabled better modeling of this disorder. Methods: Having previously studied the phenotype of dentate gyrus granule neurons, we turned our attention to studying the phenotype of CA3 hippocampal pyramidal neurons of 6 patients with BD compared with 4 control individuals. We used patch clamp and quantitative polymerase chain reaction to measure electrophysiological features and RNA expression by specific channel genes. Results: We found that BD CA3 neurons were hyperexcitable only when they were derived from patients who responded to lithium; they featured sustained activity with large current injections and a large, fast after-hyperpolarization, similar to what we previously reported in dentate gyrus neurons. The higher amplitudes and faster kinetics of fast potassium currents correlated with this hyperexcitability. Further supporting the involvement of potassium currents, we observed an overexpression of KCNC1 and KCNC2 in hippocampal neurons derived from lithium responders. Applying specific potassium channel blockers diminished the hyperexcitability. Long-term lithium treatment decreased the hyperexcitability observed in the CA3 neurons derived from lithium responders while increasing sodium currents and reducing fast potassium currents. When differentiating this cohort into spinal motor neurons, we did not observe any changes in the excitability of BD motor neurons compared with control motor neurons. Conclusions: The hyperexcitability of BD neurons is neuronal type specific with the involvement of altered potassium currents that allow for a sustained, continued firing activity.

KW - Bipolar disorder

KW - Dentate gyrus

KW - Hippocampus

KW - Hyperexcitability

KW - Motor neurons

KW - Pyramidal

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U2 - 10.1016/j.biopsych.2019.09.018

DO - 10.1016/j.biopsych.2019.09.018

M3 - Article

C2 - 31732108

AN - SCOPUS:85075333514

SN - 0006-3223

VL - 88

SP - 139

EP - 149

JO - Biological Psychiatry

JF - Biological Psychiatry

IS - 2

ER -

Mechanisms Underlying the Hyperexcitability of CA3 and Dentate Gyrus Hippocampal Neurons Derived From Patients With Bipolar Disorder

Abstract

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