Marine invertebrates cross phyla comparisons reveal highly conserved immune machinery

Matan Oren, Guy Paz, Jacob Douek, Amalia Rosner, Keren Or Amar, Baruch Rinkevich

Research output: Contribution to journalArticlepeer-review

Abstract

Naturally occurring histocompatibility responses, following tissue-to-tissue allogeneic contacts, are common among numerous colonial marine invertebrate taxa, including sponges, cnidarians, bryozoans and ascidians. These responses, often culminating in either tissue fusions or rejections, activate a wide array of innate immune components. By comparing two allorejection EST libraries, developed from alloincompatible challenged colonies of the stony coral Stylophora pistillata and the ascidian Botryllus schlosseri, we revealed a common basis for innate immunity in these two evolutionary distant species. Two prominent genes within this common basis were the immunophilins, Cyclophilin A (CypA) and FK506-binding protein (FKBP). In situ hybridizations revealed that mRNA expression of the coral and ascidian immunophilins was restricted to specific allorecognition effector cell populations (nematoblasts and nematocytes in the coral and morula cells in the ascidian). The expressions were limited to only some of the effector cells within a population, disclosing disparities in numbers and location between naïve colonies and their immune challenged counterparts. Administration of the immunosuppression drug Cyclosporine-A during ascidian's allogeneic assays inhibited both fusion and rejection reactions, probably through the inhibition of ascidian's immunocytes (morula cells) movement and activation. Our results, together with previous published data, depict an immunophilins-based immune mechanism, which is similarly activated in allogeneic responses of distantly related animals from sponges to humans.

Original languageEnglish
Pages (from-to)484-495
Number of pages12
JournalImmunobiology
Volume218
Issue number4
DOIs
StatePublished - Apr 2013
Externally publishedYes

Bibliographical note

Funding Information:
We thank Elizabeth Moiseeva for excellent histology and Marie-line Escande for EM preparation. This study was supported by Marine Genomics Europe Network of Excellence (EDD Node) and by the Israel Science Foundation ( 1342/08 and 68/10 ).

Keywords

  • Allorecognition
  • Botryllus schlosseri
  • Histocompatibility
  • Immune response
  • Immunocytes
  • Immunophilins
  • Immunosuppression
  • Marine invertebrates
  • Stylophora pistillata

ASJC Scopus subject areas

  • Hematology
  • Immunology and Allergy
  • Immunology

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