LTP in the dentate gyrus is associated with a persistent NMDA receptor-dependent enhancement of synaptosomal glutamate release

Laura Canevari; Gal Richter-Levin; T. V.P. Bliss

doi:10.1016/0006-8993(94)91720-5

LTP in the dentate gyrus is associated with a persistent NMDA receptor-dependent enhancement of synaptosomal glutamate release

Laura Canevari, Gal Richter-Levin, T. V.P. Bliss

Research output: Contribution to journal › Article › peer-review

Abstract

LTP was induced unilaterally in the dentate gyrus of the anaesthetized rat by tetanic stimulation of the perforant path. Two hours after tetanization synaptosomes were prepared from control and potentiated sides, and the depolarization-induced release of endogenous amino acid neurotransmitters was compared in the two groups, using 4-aminopyridine, potassium or veratridine as depolarizing agents. Glutamate release was greater in synaptosomes from potentiated than control tissue with all three depolarizing agents, but the highest release from control synaptosomes and the most significant enhancement of release from potentiated synaptosomes was seen with veratridine. The tetanus-induced enhancement of veratridine-induced glutamate release was prevented by pretreatment with MK-801. The results indicate that the induction of LTP in the dentate gyrus is accompanied by a persistent enhancement in the ability of synaptosomes to release glutamate in response to a depolarizing stimulus.

Original language	English
Pages (from-to)	115-117
Number of pages	3
Journal	Brain Research
Volume	667
Issue number	1
DOIs	https://doi.org/10.1016/0006-8993(94)91720-5
State	Published - 19 Dec 1994
Externally published	Yes

Bibliographical note

Funding Information:
This work was supported by grants from EEC (L.C.) and HFSP (G.R.-L.).

Keywords

4-Aminopyridine
Long-term potentiation
MK-801
Potassium
Veratridine

ASJC Scopus subject areas

General Neuroscience
Molecular Biology
Clinical Neurology
Developmental Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/0006-8993(94)91720-5

Cite this

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title = "LTP in the dentate gyrus is associated with a persistent NMDA receptor-dependent enhancement of synaptosomal glutamate release",

abstract = "LTP was induced unilaterally in the dentate gyrus of the anaesthetized rat by tetanic stimulation of the perforant path. Two hours after tetanization synaptosomes were prepared from control and potentiated sides, and the depolarization-induced release of endogenous amino acid neurotransmitters was compared in the two groups, using 4-aminopyridine, potassium or veratridine as depolarizing agents. Glutamate release was greater in synaptosomes from potentiated than control tissue with all three depolarizing agents, but the highest release from control synaptosomes and the most significant enhancement of release from potentiated synaptosomes was seen with veratridine. The tetanus-induced enhancement of veratridine-induced glutamate release was prevented by pretreatment with MK-801. The results indicate that the induction of LTP in the dentate gyrus is accompanied by a persistent enhancement in the ability of synaptosomes to release glutamate in response to a depolarizing stimulus.",

keywords = "4-Aminopyridine, Long-term potentiation, MK-801, Potassium, Veratridine",

author = "Laura Canevari and Gal Richter-Levin and Bliss, {T. V.P.}",

note = "Funding Information: This work was supported by grants from EEC (L.C.) and HFSP (G.R.-L.).",

year = "1994",

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AU - Canevari, Laura

AU - Richter-Levin, Gal

AU - Bliss, T. V.P.

N1 - Funding Information: This work was supported by grants from EEC (L.C.) and HFSP (G.R.-L.).

PY - 1994/12/19

Y1 - 1994/12/19

N2 - LTP was induced unilaterally in the dentate gyrus of the anaesthetized rat by tetanic stimulation of the perforant path. Two hours after tetanization synaptosomes were prepared from control and potentiated sides, and the depolarization-induced release of endogenous amino acid neurotransmitters was compared in the two groups, using 4-aminopyridine, potassium or veratridine as depolarizing agents. Glutamate release was greater in synaptosomes from potentiated than control tissue with all three depolarizing agents, but the highest release from control synaptosomes and the most significant enhancement of release from potentiated synaptosomes was seen with veratridine. The tetanus-induced enhancement of veratridine-induced glutamate release was prevented by pretreatment with MK-801. The results indicate that the induction of LTP in the dentate gyrus is accompanied by a persistent enhancement in the ability of synaptosomes to release glutamate in response to a depolarizing stimulus.

AB - LTP was induced unilaterally in the dentate gyrus of the anaesthetized rat by tetanic stimulation of the perforant path. Two hours after tetanization synaptosomes were prepared from control and potentiated sides, and the depolarization-induced release of endogenous amino acid neurotransmitters was compared in the two groups, using 4-aminopyridine, potassium or veratridine as depolarizing agents. Glutamate release was greater in synaptosomes from potentiated than control tissue with all three depolarizing agents, but the highest release from control synaptosomes and the most significant enhancement of release from potentiated synaptosomes was seen with veratridine. The tetanus-induced enhancement of veratridine-induced glutamate release was prevented by pretreatment with MK-801. The results indicate that the induction of LTP in the dentate gyrus is accompanied by a persistent enhancement in the ability of synaptosomes to release glutamate in response to a depolarizing stimulus.

KW - 4-Aminopyridine

KW - Long-term potentiation

KW - MK-801

KW - Potassium

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SN - 0006-8993

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ER -

LTP in the dentate gyrus is associated with a persistent NMDA receptor-dependent enhancement of synaptosomal glutamate release

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

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