LTP in the dentate gyrus is associated with a persistent NMDA receptor-dependent enhancement of synaptosomal glutamate release

Laura Canevari, Gal Richter-Levin, T. V.P. Bliss

Research output: Contribution to journalArticlepeer-review

Abstract

LTP was induced unilaterally in the dentate gyrus of the anaesthetized rat by tetanic stimulation of the perforant path. Two hours after tetanization synaptosomes were prepared from control and potentiated sides, and the depolarization-induced release of endogenous amino acid neurotransmitters was compared in the two groups, using 4-aminopyridine, potassium or veratridine as depolarizing agents. Glutamate release was greater in synaptosomes from potentiated than control tissue with all three depolarizing agents, but the highest release from control synaptosomes and the most significant enhancement of release from potentiated synaptosomes was seen with veratridine. The tetanus-induced enhancement of veratridine-induced glutamate release was prevented by pretreatment with MK-801. The results indicate that the induction of LTP in the dentate gyrus is accompanied by a persistent enhancement in the ability of synaptosomes to release glutamate in response to a depolarizing stimulus.

Original languageEnglish
Pages (from-to)115-117
Number of pages3
JournalBrain Research
Volume667
Issue number1
DOIs
StatePublished - 19 Dec 1994
Externally publishedYes

Bibliographical note

Funding Information:
This work was supported by grants from EEC (L.C.) and HFSP (G.R.-L.).

Keywords

  • 4-Aminopyridine
  • Long-term potentiation
  • MK-801
  • Potassium
  • Veratridine

ASJC Scopus subject areas

  • Clinical Neurology
  • Molecular Biology
  • General Neuroscience
  • Developmental Biology

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