LTP but not seizure is associated with up-regulation of AKAP-150

A. Génin, P. French, V. Doyère, S. Davis, M. L. Errington, M. Maroun, T. Stean, B. Truchet, M. Webber, T. Wills, G. Richter-Levin, G. Sanger, S. P. Hunt, J. Mallet, S. Laroche, T. V.P. Bliss, Vincent O'Connor

Research output: Contribution to journalArticlepeer-review


We have used differential display to profile and compare the mRNAs expressed in the hippocampus of freely moving animals after the induction of long-term potentiation (LTP) at the perforant path-dentate gyrus synapse with control rats receiving low-frequency stimulation. We have combined this with in situ hybridization and have identified A-kinase anchoring protein of 150 kDa (AKAP-150) as a gene selectively up-regulated during the maintenance phase of LTP. AKAP-150 mRNA has a biphasic modulation in the dentate gyrus following the induction of LTP. The expression of AKAP-150 was 29% lower than stimulated controls 1 h after the induction of LTR Its expression was enhanced 3 (50%), 6 (239%) and 12 h (210%) after induction, returning to control levels by 24 h postinduction. The NMDA receptor antagonist CPP blocked the tetanus-induced modulation of AKAP-150 expression. Interestingly, strong generalized stimulation produced by electroconvulsive shock did not increase the expression of AKAP-150. This implies that the AKAP-150 harbours a novel property of selective responsiveness to the stimulation patterns that trigger NMDA-dependent LTP in vivo its selective up-regulation during LTP and its identified functions as a scaffold for protein kinase A, protein kinase C, calmodulin, calcineurin and ionotropic glutamate receptors suggest that AKAP-150 encodes is an important effector protein in the expression of late LTP.

Original languageEnglish
Pages (from-to)331-340
Number of pages10
JournalEuropean Journal of Neuroscience
Issue number2
StatePublished - Jan 2003


  • Differential display
  • ECS
  • Gene expression
  • Hippocampus
  • Rat
  • Synaptic plasticity

ASJC Scopus subject areas

  • General Neuroscience


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