Long-term memory is maintained by continuous activity of Arp2/3 in lateral amygdala

Sreetama Basu, Jessica M. Alapin, Monica Dines, Raphael Lamprecht

Research output: Contribution to journalArticlepeer-review


Evidence indicates that long-term memory formation involves alterations in synaptic efficacy produced by modifications in neural transmission and morphology. However, it is not clear how such changes induced by learning, that encode memory, are maintained over long period of time to preserve long-term memory. It has been shown that the actin nucleating protein Arp2/3 is essential for supporting neuronal morphology and synaptic transmission. We therefore hypothesized that continuous Arp2/3 activity is needed to maintain long-term memory over time. To test this hypothesis we microinjected into lateral amygdala (LA) of rats CK-666, a specific inhibitor of Arp2/3, two days after fear conditioning and tested the effect on long-term fear memory maintenance a day afterward. We found that injection of CK-666 two days after training abolished fear conditioning memory. Fear conditioning could be formed when a control compound CK-689 was applied two days after training. Microinjection of CK-666 a day before fear conditioning training had no effect on fear conditioning learning and long-term memory formation. We revealed that Arp2/3 is also needed to maintain long-term conditioned taste aversion (CTA) memory in LA. Microinjection of CK-666 two days after CTA training impaired long-term memory tested a day afterwards. We conclude that continuous activity of Arp2/3 in LA is essential for the maintenance of long-term memory.

Original languageEnglish
Article number107115
JournalNeurobiology of Learning and Memory
StatePublished - Jan 2020

Bibliographical note

Publisher Copyright:
© 2019 Elsevier Inc.


  • Actin cytoskeleton
  • Amygdala
  • Arp2/3
  • Conditioned taste aversion
  • Fear conditioning
  • Memory maintenance

ASJC Scopus subject areas

  • Experimental and Cognitive Psychology
  • Cognitive Neuroscience
  • Behavioral Neuroscience


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