Leucine transport into brush border membrane vesicles from guts of Leptinotarsa decemlineata and Manduca sexta

M. Reuveni; Y. S. Hong; P. E. Dunn; J. J. Neal

doi:10.1016/0300-9629(93)90315-U

Leucine transport into brush border membrane vesicles from guts of Leptinotarsa decemlineata and Manduca sexta

M. Reuveni, Y. S. Hong, P. E. Dunn, J. J. Neal

Research output: Contribution to journal › Article › peer-review

Abstract

1. 1. Compared to crude homogenates, Manduca sexta brush border membrane vesicles (BBMV) had increased specific activity of marker enzymes leucine aminopeptidase and alkaline phosphatase while Leptinotarsa decemlineata BBMV had increased leucine aminopeptidase specific activity and decreased alkaline phosphatase specific activity. 2. 2. L. decemlineata BBMV were impermeable to a proton gradient while M. sexta BBMV sustained an alkali cation gradient. 3. 3. Both K⁺-stimulated and K⁺-independent unidirectional leucine transport pathways were identified in M. sexta BBMV while only cation-independent unidirectional leucine transport was identified in L. decemlineata BBMV.

Original language	English
Pages (from-to)	267-272
Number of pages	6
Journal	Comparative Biochemistry and Physiology -Part A : Molecular and Integrative Physiology
Volume	104
Issue number	2
DOIs	https://doi.org/10.1016/0300-9629(93)90315-U
State	Published - Feb 1993
Externally published	Yes

ASJC Scopus subject areas

Physiology

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10.1016/0300-9629(93)90315-U

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title = "Leucine transport into brush border membrane vesicles from guts of Leptinotarsa decemlineata and Manduca sexta",

abstract = "1. 1. Compared to crude homogenates, Manduca sexta brush border membrane vesicles (BBMV) had increased specific activity of marker enzymes leucine aminopeptidase and alkaline phosphatase while Leptinotarsa decemlineata BBMV had increased leucine aminopeptidase specific activity and decreased alkaline phosphatase specific activity. 2. 2. L. decemlineata BBMV were impermeable to a proton gradient while M. sexta BBMV sustained an alkali cation gradient. 3. 3. Both K+-stimulated and K+-independent unidirectional leucine transport pathways were identified in M. sexta BBMV while only cation-independent unidirectional leucine transport was identified in L. decemlineata BBMV.",

author = "M. Reuveni and Hong, {Y. S.} and Dunn, {P. E.} and Neal, {J. J.}",

year = "1993",

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doi = "10.1016/0300-9629(93)90315-U",

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T1 - Leucine transport into brush border membrane vesicles from guts of Leptinotarsa decemlineata and Manduca sexta

AU - Reuveni, M.

AU - Hong, Y. S.

AU - Dunn, P. E.

AU - Neal, J. J.

PY - 1993/2

Y1 - 1993/2

N2 - 1. 1. Compared to crude homogenates, Manduca sexta brush border membrane vesicles (BBMV) had increased specific activity of marker enzymes leucine aminopeptidase and alkaline phosphatase while Leptinotarsa decemlineata BBMV had increased leucine aminopeptidase specific activity and decreased alkaline phosphatase specific activity. 2. 2. L. decemlineata BBMV were impermeable to a proton gradient while M. sexta BBMV sustained an alkali cation gradient. 3. 3. Both K+-stimulated and K+-independent unidirectional leucine transport pathways were identified in M. sexta BBMV while only cation-independent unidirectional leucine transport was identified in L. decemlineata BBMV.

AB - 1. 1. Compared to crude homogenates, Manduca sexta brush border membrane vesicles (BBMV) had increased specific activity of marker enzymes leucine aminopeptidase and alkaline phosphatase while Leptinotarsa decemlineata BBMV had increased leucine aminopeptidase specific activity and decreased alkaline phosphatase specific activity. 2. 2. L. decemlineata BBMV were impermeable to a proton gradient while M. sexta BBMV sustained an alkali cation gradient. 3. 3. Both K+-stimulated and K+-independent unidirectional leucine transport pathways were identified in M. sexta BBMV while only cation-independent unidirectional leucine transport was identified in L. decemlineata BBMV.

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U2 - 10.1016/0300-9629(93)90315-U

DO - 10.1016/0300-9629(93)90315-U

M3 - Article

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SN - 0300-9629

VL - 104

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JO - Comparative Biochemistry and Physiology -Part A : Molecular and Integrative Physiology

JF - Comparative Biochemistry and Physiology -Part A : Molecular and Integrative Physiology

IS - 2

ER -

Leucine transport into brush border membrane vesicles from guts of Leptinotarsa decemlineata and Manduca sexta

Abstract

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