Large-scale, high-density (up to 512 channels) recording of local circuits in behaving animals

Antal Berényi, Zoltán Somogyvári, Anett J. Nagy, Lisa Roux, John D. Long, Shigeyoshi Fujisawa, Eran Stark, Anthony Leonardo, Timothy D. Harris, György Buzsáki

Research output: Contribution to journalArticlepeer-review


Monitoring representative fractions of neurons from multiple brain circuits in behaving animals is necessary for understanding neuronal computation. Here, we describe a system that allows high-channel-count recordings from a small volume of neuronal tissue using a lightweight signal multiplexing headstage that permits free behavior of small rodents. The system integrates multishank, high-density recording silicon probes, ultraflexible interconnects, and a miniaturized microdrive. These improvements allowed for simultaneous recordings of local field potentials and unit activity from hundreds of sites without confining free movements of the animal. The advantages of large-scale recordings are illustrated by determining the electroanatomic boundaries of layers and regions in the hippocampus and neocortex and constructing a circuit diagram of functional connections among neurons in real anatomic space. These methods will allow the investigation of circuit operations and behavior-dependent interregional interactions for testing hypotheses of neural networks and brain function.

Original languageEnglish
Pages (from-to)1132-1149
Number of pages18
JournalJournal of Neurophysiology
Issue number5
StatePublished - 1 Mar 2014
Externally publishedYes


  • Behaving rats and mice
  • Local field potential
  • Monosynaptic connections
  • Unit firing

ASJC Scopus subject areas

  • Neuroscience (all)
  • Physiology


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