Izhakiella capsodis gen. nov., sp. nov., in the family Enterobacteriaceae, isolated from the mirid bug Capsodes infuscatus

Gram-stain-negative, oxidase-negative, facultatively anaerobic, motile, rod-shaped, nonpigmented bacterial strains (N6PO6^T, N8PO1 and N8PI1) were isolated from the mirid bug Capsodes infuscatus captured on Asphodelus aestivus plants. The 16S rRNA gene sequences of the strains shared 94.7–95.7% similarity with species of the genus Pantoea and 95.6% or less with species from other genera in the family Enterobacteriaceae. A polyphasic approach that included determination of phenotypic properties and phylogenetic analysis based on 16S rRNA, rpoB, gyrB and atpD gene sequences supported the classification of strains N6PO6^T, N8PO1 and N8PI1 as representing a novel species of a new genus in the family Enterobacteriaceae. Strain N6PO6^T, and the two reference strains of the novel species, grew at 1–37 °C, and in the presence of NaCl (up to 7.5%, w/v) and sucrose (up to 60%). Their major cellular fatty acids were C_{16: 0}, C_{17: 0} cyclo, C₁₈:₁ω7c, summed feature 2 (C_{14: 0} 3-OH and/or iso-C_{16: 1} I) and summed feature 3 (C_{16: 1} ω 7c and/or iso-C_{15: 0} 2-OH). The DNA G+C content of strain N6PO6^T was 49.9 mol%. On the basis of phenotypic properties and phylogenetic distinctiveness, the mirid bug isolates are classified as representing a novel species in a new genus Izhakiella, in the family Enterobacteriaceae, for which the name Izhakiella capsodis gen. nov., sp. nov. is proposed. The type strain of Izhakiella capsodis is N6PO6^T (=LMG 28430^T=DSM 29293^T).