Isolation of microsatellites from a bovine YAC clone harbouring the SOD1 gene

M. Band; A. Eggen; M. D. Bishop; M. Ron

doi:10.1111/j.1365-2052.1997.00158.x

Isolation of microsatellites from a bovine YAC clone harbouring the SOD1 gene

M. Band, A. Eggen, M. D. Bishop, M. Ron

Research output: Contribution to journal › Article › peer-review

Abstract

A bovine yeast artificial chromosome (YAC) clone containing the superoxide dismutase 1 (SOD1) gene was used as a template for polymerase chain reaction (PCR) amplification using a conserved short interspersed nuclear element (SINE) primer. Two highly polymorphic microsatellites with nine and eight alleles were isolated and mapped by linkage analysis to the centromeric region of BTA1. These microsatellites will be used in the construction of a genetic and physical map of the SOD1 region towards positional cloning of the polled gene.

Original language	English
Pages (from-to)	363-366
Number of pages	4
Journal	Animal Genetics
Volume	28
Issue number	5
DOIs	https://doi.org/10.1111/j.1365-2052.1997.00158.x
State	Published - Oct 1997
Externally published	Yes

Keywords

Bovine
Polled
SOD1
YAC

ASJC Scopus subject areas

Animal Science and Zoology
Genetics

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10.1111/j.1365-2052.1997.00158.x

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abstract = "A bovine yeast artificial chromosome (YAC) clone containing the superoxide dismutase 1 (SOD1) gene was used as a template for polymerase chain reaction (PCR) amplification using a conserved short interspersed nuclear element (SINE) primer. Two highly polymorphic microsatellites with nine and eight alleles were isolated and mapped by linkage analysis to the centromeric region of BTA1. These microsatellites will be used in the construction of a genetic and physical map of the SOD1 region towards positional cloning of the polled gene.",

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author = "M. Band and A. Eggen and Bishop, \{M. D.\} and M. Ron",

year = "1997",

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AU - Eggen, A.

AU - Bishop, M. D.

AU - Ron, M.

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AB - A bovine yeast artificial chromosome (YAC) clone containing the superoxide dismutase 1 (SOD1) gene was used as a template for polymerase chain reaction (PCR) amplification using a conserved short interspersed nuclear element (SINE) primer. Two highly polymorphic microsatellites with nine and eight alleles were isolated and mapped by linkage analysis to the centromeric region of BTA1. These microsatellites will be used in the construction of a genetic and physical map of the SOD1 region towards positional cloning of the polled gene.

KW - Bovine

KW - Polled

KW - SOD1

KW - YAC

UR - https://www.scopus.com/pages/publications/0031258859

U2 - 10.1111/j.1365-2052.1997.00158.x

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EP - 366

JO - Animal Genetics

JF - Animal Genetics

IS - 5

ER -

Isolation of microsatellites from a bovine YAC clone harbouring the SOD1 gene

Abstract

Keywords

ASJC Scopus subject areas

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