Isolation of Marine Paracoccus sp. Ss63 from the Sponge Sarcotragus sp. and Characterization of its Quorum-Sensing Chemical-Signaling Molecules by LC-MS/MS Analysis

Kumar Saurav; Ilia Burgsdorf; Roberta Teta; Germana Esposito; Rinat Bar-Shalom; Valeria Costantino; Laura Steindler

doi:10.1002/ijch.201600003

Isolation of Marine Paracoccus sp. Ss63 from the Sponge Sarcotragus sp. and Characterization of its Quorum-Sensing Chemical-Signaling Molecules by LC-MS/MS Analysis

Kumar Saurav, Ilia Burgsdorf, Roberta Teta, Germana Esposito, Rinat Bar-Shalom, Valeria Costantino, Laura Steindler

Department of Marine Biology

Research output: Contribution to journal › Article › peer-review

Abstract

Gram-negative bacteria often use N-acyl homoserine lactones (AHLs) as quorum-sensing (QS) signal molecules. The present study aimed to identify and characterize the production of AHLs by Paracoccus sp. Ss63, which was isolated from the Mediterranean sponge Sarcotragus sp. High performance liquid chromatography - high resolution tandem mass spectrometry (LC-HRMS/MS) provided evidence for the production, by Paracoccus sp. Ss63, of twelve saturated long-chain AHLs (3-OH-C10-HSL, 3-OH-C12-HSL, C10-HSL, 3-OH-C14-HSL, C12-HSL, 3-O-C16-HSL, 3-OH-C16-HSL, C14-HSL, 3-OH-C18-HSL, C16-HSL, 3-O-C18-HSL, and C18-HSL), along with four putative unsaturated long-chain AHLs (C10: 1-HSL, 3-OH-C18: 1-HSL, C16: 1-HSL, and C18: 1-HSL). The distribution of Paracoccus sp. Ss63 in different sponge species, as well as in seawater and marine sediment samples, suggests a mixed lifestyle for this bacterium, i.e.; free-living and host-associated. The high pH of seawater is likely to inactivate the AHL signal molecules, and limit the possibility of AHLs to accumulate to the concentration required for QS-based gene regulation. We hypothesize that Paracoccus sp. Ss63 utilizes quorum sensing to sense and respond to the different environments it experiences, with an active QS only when found inside enclosed niches (host-associated and at lower pH).

Original language	English
Pages (from-to)	330-340
Number of pages	11
Journal	Israel Journal of Chemistry
Volume	56
Issue number	5
DOIs	https://doi.org/10.1002/ijch.201600003
State	Published - 1 May 2016

Bibliographical note

Funding Information:
This study was supported by the Bilateral Italy?Israel R&D grant, MOST grant #10705?3 to L. S. The work was partially supported by the European Union's Seventh Framework Programme (FP7) 2007-2013 under Grant Agreement No. 311848 (Bluegenics). K. S. had a postdoctoral fellowship from the Israeli Council for Higher Education (VATAT) and University of Haifa. K. S.' postdoctoral fellowship was also supported by the "Universita degli Studi di Napoli Federico II" bilateral agreement Haifa?Naples 2015-2018. Samples were collected in compliance with the 40246/2014 permit from the Israel Nature and National Parks Protection Authority. K. S. is also thankful to Ms. Maya Britstein, Mr. Ilia Burgsdorf, and Dr. Markus Haber for assistance with sponge collection. Amplicon sequencing data from sponge and environmental microbiomes was derived from the Earth Microbiome Project (www.earthmicrobiome.org), all amplicon and metadata of project EMP 1 is public.

Publisher Copyright:
© 2016 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

Keywords

Sarcotragus
free-living
host-associated
isolation
lactones
marine sponges
quorum sensing

ASJC Scopus subject areas

Chemistry (all)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1002/ijch.201600003

Cite this

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title = "Isolation of Marine Paracoccus sp. Ss63 from the Sponge Sarcotragus sp. and Characterization of its Quorum-Sensing Chemical-Signaling Molecules by LC-MS/MS Analysis",

abstract = "Gram-negative bacteria often use N-acyl homoserine lactones (AHLs) as quorum-sensing (QS) signal molecules. The present study aimed to identify and characterize the production of AHLs by Paracoccus sp. Ss63, which was isolated from the Mediterranean sponge Sarcotragus sp. High performance liquid chromatography - high resolution tandem mass spectrometry (LC-HRMS/MS) provided evidence for the production, by Paracoccus sp. Ss63, of twelve saturated long-chain AHLs (3-OH-C10-HSL, 3-OH-C12-HSL, C10-HSL, 3-OH-C14-HSL, C12-HSL, 3-O-C16-HSL, 3-OH-C16-HSL, C14-HSL, 3-OH-C18-HSL, C16-HSL, 3-O-C18-HSL, and C18-HSL), along with four putative unsaturated long-chain AHLs (C10: 1-HSL, 3-OH-C18: 1-HSL, C16: 1-HSL, and C18: 1-HSL). The distribution of Paracoccus sp. Ss63 in different sponge species, as well as in seawater and marine sediment samples, suggests a mixed lifestyle for this bacterium, i.e.; free-living and host-associated. The high pH of seawater is likely to inactivate the AHL signal molecules, and limit the possibility of AHLs to accumulate to the concentration required for QS-based gene regulation. We hypothesize that Paracoccus sp. Ss63 utilizes quorum sensing to sense and respond to the different environments it experiences, with an active QS only when found inside enclosed niches (host-associated and at lower pH).",

keywords = "Sarcotragus, free-living, host-associated, isolation, lactones, marine sponges, quorum sensing",

author = "Kumar Saurav and Ilia Burgsdorf and Roberta Teta and Germana Esposito and Rinat Bar-Shalom and Valeria Costantino and Laura Steindler",

note = "Funding Information: This study was supported by the Bilateral Italy?Israel R&D grant, MOST grant #10705?3 to L. S. The work was partially supported by the European Union's Seventh Framework Programme (FP7) 2007-2013 under Grant Agreement No. 311848 (Bluegenics). K. S. had a postdoctoral fellowship from the Israeli Council for Higher Education (VATAT) and University of Haifa. K. S.' postdoctoral fellowship was also supported by the {"}Universita degli Studi di Napoli Federico II{"} bilateral agreement Haifa?Naples 2015-2018. Samples were collected in compliance with the 40246/2014 permit from the Israel Nature and National Parks Protection Authority. K. S. is also thankful to Ms. Maya Britstein, Mr. Ilia Burgsdorf, and Dr. Markus Haber for assistance with sponge collection. Amplicon sequencing data from sponge and environmental microbiomes was derived from the Earth Microbiome Project (www.earthmicrobiome.org), all amplicon and metadata of project EMP 1 is public. Publisher Copyright: {\textcopyright} 2016 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.",

year = "2016",

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doi = "10.1002/ijch.201600003",

language = "English",

volume = "56",

pages = "330--340",

journal = "Israel Journal of Chemistry",

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T1 - Isolation of Marine Paracoccus sp. Ss63 from the Sponge Sarcotragus sp. and Characterization of its Quorum-Sensing Chemical-Signaling Molecules by LC-MS/MS Analysis

AU - Saurav, Kumar

AU - Burgsdorf, Ilia

AU - Teta, Roberta

AU - Esposito, Germana

AU - Bar-Shalom, Rinat

AU - Costantino, Valeria

AU - Steindler, Laura

N1 - Funding Information: This study was supported by the Bilateral Italy?Israel R&D grant, MOST grant #10705?3 to L. S. The work was partially supported by the European Union's Seventh Framework Programme (FP7) 2007-2013 under Grant Agreement No. 311848 (Bluegenics). K. S. had a postdoctoral fellowship from the Israeli Council for Higher Education (VATAT) and University of Haifa. K. S.' postdoctoral fellowship was also supported by the "Universita degli Studi di Napoli Federico II" bilateral agreement Haifa?Naples 2015-2018. Samples were collected in compliance with the 40246/2014 permit from the Israel Nature and National Parks Protection Authority. K. S. is also thankful to Ms. Maya Britstein, Mr. Ilia Burgsdorf, and Dr. Markus Haber for assistance with sponge collection. Amplicon sequencing data from sponge and environmental microbiomes was derived from the Earth Microbiome Project (www.earthmicrobiome.org), all amplicon and metadata of project EMP 1 is public. Publisher Copyright: © 2016 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

PY - 2016/5/1

Y1 - 2016/5/1

N2 - Gram-negative bacteria often use N-acyl homoserine lactones (AHLs) as quorum-sensing (QS) signal molecules. The present study aimed to identify and characterize the production of AHLs by Paracoccus sp. Ss63, which was isolated from the Mediterranean sponge Sarcotragus sp. High performance liquid chromatography - high resolution tandem mass spectrometry (LC-HRMS/MS) provided evidence for the production, by Paracoccus sp. Ss63, of twelve saturated long-chain AHLs (3-OH-C10-HSL, 3-OH-C12-HSL, C10-HSL, 3-OH-C14-HSL, C12-HSL, 3-O-C16-HSL, 3-OH-C16-HSL, C14-HSL, 3-OH-C18-HSL, C16-HSL, 3-O-C18-HSL, and C18-HSL), along with four putative unsaturated long-chain AHLs (C10: 1-HSL, 3-OH-C18: 1-HSL, C16: 1-HSL, and C18: 1-HSL). The distribution of Paracoccus sp. Ss63 in different sponge species, as well as in seawater and marine sediment samples, suggests a mixed lifestyle for this bacterium, i.e.; free-living and host-associated. The high pH of seawater is likely to inactivate the AHL signal molecules, and limit the possibility of AHLs to accumulate to the concentration required for QS-based gene regulation. We hypothesize that Paracoccus sp. Ss63 utilizes quorum sensing to sense and respond to the different environments it experiences, with an active QS only when found inside enclosed niches (host-associated and at lower pH).

AB - Gram-negative bacteria often use N-acyl homoserine lactones (AHLs) as quorum-sensing (QS) signal molecules. The present study aimed to identify and characterize the production of AHLs by Paracoccus sp. Ss63, which was isolated from the Mediterranean sponge Sarcotragus sp. High performance liquid chromatography - high resolution tandem mass spectrometry (LC-HRMS/MS) provided evidence for the production, by Paracoccus sp. Ss63, of twelve saturated long-chain AHLs (3-OH-C10-HSL, 3-OH-C12-HSL, C10-HSL, 3-OH-C14-HSL, C12-HSL, 3-O-C16-HSL, 3-OH-C16-HSL, C14-HSL, 3-OH-C18-HSL, C16-HSL, 3-O-C18-HSL, and C18-HSL), along with four putative unsaturated long-chain AHLs (C10: 1-HSL, 3-OH-C18: 1-HSL, C16: 1-HSL, and C18: 1-HSL). The distribution of Paracoccus sp. Ss63 in different sponge species, as well as in seawater and marine sediment samples, suggests a mixed lifestyle for this bacterium, i.e.; free-living and host-associated. The high pH of seawater is likely to inactivate the AHL signal molecules, and limit the possibility of AHLs to accumulate to the concentration required for QS-based gene regulation. We hypothesize that Paracoccus sp. Ss63 utilizes quorum sensing to sense and respond to the different environments it experiences, with an active QS only when found inside enclosed niches (host-associated and at lower pH).

KW - Sarcotragus

KW - free-living

KW - host-associated

KW - isolation

KW - lactones

KW - marine sponges

KW - quorum sensing

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VL - 56

SP - 330

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JO - Israel Journal of Chemistry

JF - Israel Journal of Chemistry

IS - 5

ER -

Isolation of Marine Paracoccus sp. Ss63 from the Sponge Sarcotragus sp. and Characterization of its Quorum-Sensing Chemical-Signaling Molecules by LC-MS/MS Analysis

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

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