Isolation and characterization of a novel Lambda-like phage infecting the bloom-forming cyanobacteria Cylindrospermopsis raciborskii

Emmanuelle Laloum, Esther Cattan-Tsaushu, Daniel A. Schwartz, Hanaa Shaalan, Hagay Enav, Dikla Kolan, Sarit Avrani

Research output: Contribution to journalArticlepeer-review

Abstract

Cylindrospermopsis raciborskii is a central bloom-forming cyanobacteria. However, despite its ecological significance, little is known of its interactions with the phages that infect it. Currently, only a single sequenced genome of a Cylindrospermopsis-infecting phage is publicly available. Here we describe the isolation and characterization of Cr-LKS3, a second phage infecting Cylindrospermopsis. Cr-LKS3 is a siphovirus with a higher genome similarity to prophages within heterotrophic bacteria genomes than to any other cyanophage/cyano-prophage, suggesting that it represents a novel cyanophage group. The function, order and orientation of the 72 genes in the Cr-LKS3 genome are highly similar to those of Escherichia virus Lambda (hereafter Lambda), despite the very low sequence similarity between these phages, showing high evolutionary convergence despite the substantial difference in host characteristics. Similarly to Lambda, the genome of Cr-LKS3 contains various genes that are known to be central to lysogeny, suggesting it can enter a lysogenic cycle. Cr-LKS3 has a unique ability to infect a host with a dramatically different GC content, without carrying any tRNA genes to compensate for this difference. This ability, together with its potential lysogenic lifestyle shed light on the complex interactions between C. raciborskii and its phages.

Original languageEnglish
JournalEnvironmental Microbiology
DOIs
StateAccepted/In press - 2022

Bibliographical note

Funding Information:
We thank O. Hadas for the . culture provided to us; S. Ninio for providing us the water sample from which Cr‐LKS3 was isolated; T. Dagan and R. Hershberg for comments on the manuscript. This work was supported by a GIF YOUNG grant (no. I‐2505‐413.13/2018) and a startup BSF grant (no. 2017056) to S.A., and by the National Science Foundation DEB (no. 1934554) to D.A.S. Additionally, this research was supported in part by Lilly Endowment, through its support for the Indiana University Pervasive Technology Institute. C raciborskii

Publisher Copyright:
© 2022 The Authors. Environmental Microbiology published by Society for Applied Microbiology and John Wiley & Sons Ltd.

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

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