Interlaboratory comparison trial on cylindrospermopsin measurement

Andrea Törökné; Maria Asztalos; Maria Bánkiné; Heike Bickel; Georg Borbély; Shmuel Carmeli; Geoffrey A. Codd; Jutta Fastner; Qiong Huang; Andrew Humpage; James S. Metcalf; Erzsebet Rábai; Assaf Sukenik; Gyula Surányi; Gabor Vasas; Viktor Weiszfeiler

doi:10.1016/j.ab.2004.05.036

Interlaboratory comparison trial on cylindrospermopsin measurement

Andrea Törökné, Maria Asztalos, Maria Bánkiné, Heike Bickel, Georg Borbély, Shmuel Carmeli, Geoffrey A. Codd, Jutta Fastner, Qiong Huang, Andrew Humpage, James S. Metcalf, Erzsebet Rábai, Assaf Sukenik, Gyula Surányi, Gabor Vasas, Viktor Weiszfeiler

Research output: Contribution to journal › Article › peer-review

Abstract

The hepatotoxin cylindrospermopsin (CYN) is a potent inhibitor of protein synthesis in mammalian cells. It is produced by freshwater cyanobacterial blooms in countries such as Australia, the United States, Israel, Thailand, and Brazil. An interlaboratory comparison was organized as a first step to evaluate the measurement of CYN in lyophilized cyanobacterial cells. Six laboratories from Europe, Israel, and Australia participated in the trial. All of the methods used for extraction of the toxin and the high-performance liquid chromatography (HPLC) analysis were satisfactory on the basis of statistical evaluation, according to ISO standards 5725-1 and -2. Further comparison of all the extraction methods by the organizer indicated that the most effective extraction procedure used 5% formic acid to prevent interference in chromatograms by contaminant compounds when analyzed using HPLC employing isocratic conditions of 5% (v/v) aqueous methanol plus 0.1% (v/v) trifluoroacetic acid as the mobile phase.

Original language	English
Pages (from-to)	280-284
Number of pages	5
Journal	Analytical Biochemistry
Volume	332
Issue number	2
DOIs	https://doi.org/10.1016/j.ab.2004.05.036
State	Published - 15 Sep 2004
Externally published	Yes

Keywords

Cyanobacteria
Cylindrospermopsin
HPLC analysis
Intercomparison

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.ab.2004.05.036

Cite this

Törökné, A., Asztalos, M., Bánkiné, M., Bickel, H., Borbély, G., Carmeli, S., Codd, G. A., Fastner, J., Huang, Q., Humpage, A., Metcalf, J. S., Rábai, E., Sukenik, A., Surányi, G., Vasas, G., & Weiszfeiler, V. (2004). Interlaboratory comparison trial on cylindrospermopsin measurement. Analytical Biochemistry, 332(2), 280-284. https://doi.org/10.1016/j.ab.2004.05.036

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abstract = "The hepatotoxin cylindrospermopsin (CYN) is a potent inhibitor of protein synthesis in mammalian cells. It is produced by freshwater cyanobacterial blooms in countries such as Australia, the United States, Israel, Thailand, and Brazil. An interlaboratory comparison was organized as a first step to evaluate the measurement of CYN in lyophilized cyanobacterial cells. Six laboratories from Europe, Israel, and Australia participated in the trial. All of the methods used for extraction of the toxin and the high-performance liquid chromatography (HPLC) analysis were satisfactory on the basis of statistical evaluation, according to ISO standards 5725-1 and -2. Further comparison of all the extraction methods by the organizer indicated that the most effective extraction procedure used 5% formic acid to prevent interference in chromatograms by contaminant compounds when analyzed using HPLC employing isocratic conditions of 5% (v/v) aqueous methanol plus 0.1% (v/v) trifluoroacetic acid as the mobile phase.",

keywords = "Cyanobacteria, Cylindrospermopsin, HPLC analysis, Intercomparison",

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doi = "10.1016/j.ab.2004.05.036",

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AU - Törökné, Andrea

AU - Asztalos, Maria

AU - Bánkiné, Maria

AU - Bickel, Heike

AU - Borbély, Georg

AU - Carmeli, Shmuel

AU - Codd, Geoffrey A.

AU - Fastner, Jutta

AU - Huang, Qiong

AU - Humpage, Andrew

AU - Metcalf, James S.

AU - Rábai, Erzsebet

AU - Sukenik, Assaf

AU - Surányi, Gyula

AU - Vasas, Gabor

AU - Weiszfeiler, Viktor

PY - 2004/9/15

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N2 - The hepatotoxin cylindrospermopsin (CYN) is a potent inhibitor of protein synthesis in mammalian cells. It is produced by freshwater cyanobacterial blooms in countries such as Australia, the United States, Israel, Thailand, and Brazil. An interlaboratory comparison was organized as a first step to evaluate the measurement of CYN in lyophilized cyanobacterial cells. Six laboratories from Europe, Israel, and Australia participated in the trial. All of the methods used for extraction of the toxin and the high-performance liquid chromatography (HPLC) analysis were satisfactory on the basis of statistical evaluation, according to ISO standards 5725-1 and -2. Further comparison of all the extraction methods by the organizer indicated that the most effective extraction procedure used 5% formic acid to prevent interference in chromatograms by contaminant compounds when analyzed using HPLC employing isocratic conditions of 5% (v/v) aqueous methanol plus 0.1% (v/v) trifluoroacetic acid as the mobile phase.

AB - The hepatotoxin cylindrospermopsin (CYN) is a potent inhibitor of protein synthesis in mammalian cells. It is produced by freshwater cyanobacterial blooms in countries such as Australia, the United States, Israel, Thailand, and Brazil. An interlaboratory comparison was organized as a first step to evaluate the measurement of CYN in lyophilized cyanobacterial cells. Six laboratories from Europe, Israel, and Australia participated in the trial. All of the methods used for extraction of the toxin and the high-performance liquid chromatography (HPLC) analysis were satisfactory on the basis of statistical evaluation, according to ISO standards 5725-1 and -2. Further comparison of all the extraction methods by the organizer indicated that the most effective extraction procedure used 5% formic acid to prevent interference in chromatograms by contaminant compounds when analyzed using HPLC employing isocratic conditions of 5% (v/v) aqueous methanol plus 0.1% (v/v) trifluoroacetic acid as the mobile phase.

KW - Cyanobacteria

KW - Cylindrospermopsin

KW - HPLC analysis

KW - Intercomparison

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JO - Analytical Biochemistry

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IS - 2

ER -

Interlaboratory comparison trial on cylindrospermopsin measurement

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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