Insula to mpfc reciprocal connectivity differentially underlies novel taste neophobic response and learning in mice

Haneen Kayyal, Sailendrakumar Kolatt Chandran, Adonis Yiannakas, Nathaniel Gould, Mohammad Khamaisy, Kobi Rosenblum

Research output: Contribution to journalArticlepeer-review


To survive in an ever-changing environment, animals must detect and learn salient information. The anterior insular cortex (aIC) and medial prefrontal cortex (mPFC) are heavily implicated in salience and novelty processing, and specifically, the processing of taste sensory information. Here, we examined the role of aIC-mPFC reciprocal connectivity in novel taste neophobia and memory formation, in mice. Using pERK and neuronal intrinsic properties as markers for neuronal activation, and retrograde AAV (rAAV) constructs for connectivity, we demonstrate a correlation between aIC-mPFC activity and novel taste experience. Furthermore, by expressing inhibitory chemogenetic receptors in these projections, we show that aIC-to-mPFC activity is necessary for both taste neophobia and its attenuation. However, activity within mPFC-to-aIC projections is essential only for the neophobic reaction but not for the learning process. These results provide an insight into the cortical circuitry needed to detect, react to-and learn salient stimuli, a process critically involved in psychiatric disorders.

Original languageEnglish
Article numbere66686
StatePublished - Jul 2021

Bibliographical note

Funding Information:
The authors would like to thank all current members of the Rosenblum lab for their help and support, to the veterinary team headed by Barak Carmi and Corina Dollingher and technical team headed by Yair Bellehsen. This research was supported by a grant from the Israel Science Foundation (ISF); ISF 946/17 and ISF 258/20 to KR. HK is a recipient of the Edmond de Rothschild’s scholarship. Graphical illustrations were created with

Publisher Copyright:
© Kayyal et al.

ASJC Scopus subject areas

  • Neuroscience (all)
  • Biochemistry, Genetics and Molecular Biology (all)
  • Immunology and Microbiology (all)


Dive into the research topics of 'Insula to mpfc reciprocal connectivity differentially underlies novel taste neophobic response and learning in mice'. Together they form a unique fingerprint.

Cite this