Inhibitory effect of ethyl acetate extract of the Shaggy Inc Cap medicinal mushroom, Coprinus comatus (Higher Basidiomycetes) fruit bodies on cell growth of human ovarian cancer

Amal Rouhana-Toubi, Solomon P. Wasser, Abed Agbarya, Fuad Fares

Research output: Contribution to journalArticlepeer-review

Abstract

In a previous study, we screened organic extracts of different mushroom mycelia and picked ethyl acetate extract of Coprinus comatus as one of the most active extracts against human ovarian cancer cells. In the current study, we extracted a dry powder of C. comatus fruit bodies using ethyl acetate and examined its effect on the viability of three cell lines originated from human ovarian cancer (ES-2, SKOV-3, and SW-626). This extract was active against all tested cell lines, in a dose-dependent manner (concentrations 50-200 μg/mL, P<0.01). In an attempt to segregate the active fraction, we subjected the extract to chromatography on a silica gel column. The effect of six different fractions and of the crude extract on the viability of ES-2 cells was examined after exposure time of 24 h. Fraction F (last eluted) was significantly more effective than crude extract in the reduction of cell viability (P<0.01). Fraction F was also significantly more active than crude extract in the reduction of viability of SKOV-3 cells. We next identified some of the compounds of fraction F (mainly fatty acids) by gas chromatography-mass spectrometry. In summary, ethyl acetate extract of C. comatus reduced viability of three lines of human ovarian cancer. Fractionation of this extract by a silica gel column enabled the selection of a fraction significantly more active than the original extract.

Original languageEnglish
Pages (from-to)457-470
Number of pages14
JournalInternational Journal of Medicinal Mushrooms
Volume15
Issue number5
DOIs
StatePublished - 2013

Keywords

  • Cell viability
  • Chromatography
  • Coprinus comatus
  • Medicinal mushrooms
  • Ovarian cancer
  • Shaggi Inc Cap medicinal mushroom

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Pharmacology
  • Drug Discovery

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