Independent localization of MAP2, CaMKIIα and β-actin RNAs in low copy numbers

Martin Mikl; Georgia Vendra; Michael A. Kiebler

doi:10.1038/embor.2011.149

Independent localization of MAP2, CaMKIIα and β-actin RNAs in low copy numbers

Martin Mikl, Georgia Vendra, Michael A. Kiebler

Research output: Contribution to journal › Article › peer-review

Abstract

Messenger RNA localization involves the assembly of ribonucleoprotein particles (RNPs) and their subsequent transport along the cytoskeleton to their final destination. Here, we provide new evidence that microtubule-associated protein 2 (MAP2), calcium/calmodulin-dependent protein kinase II (CaMKIIα) and β-actin RNAs localize to dendrites in distinct RNPs, which contain-unexpectedly-very few RNA molecules. The number of MAP2 molecules per particle is affected by synaptic activity and Staufen 2, indicating that RNP composition is tightly controlled. Our data suggest that the independent localization of individual RNAs in low copy numbers could contribute to tighter temporal and spatial control of expression in neurons and synapse-specific plasticity.

Original language	English
Pages (from-to)	1077-1084
Number of pages	8
Journal	EMBO Reports
Volume	12
Issue number	10
DOIs	https://doi.org/10.1038/embor.2011.149
State	Published - Oct 2011
Externally published	Yes

Keywords

CaMKIIa
MAP2 mRNA
RNA localization
Staufen 2
β-actin

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Genetics

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10.1038/embor.2011.149

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abstract = "Messenger RNA localization involves the assembly of ribonucleoprotein particles (RNPs) and their subsequent transport along the cytoskeleton to their final destination. Here, we provide new evidence that microtubule-associated protein 2 (MAP2), calcium/calmodulin-dependent protein kinase II (CaMKIIα) and β-actin RNAs localize to dendrites in distinct RNPs, which contain-unexpectedly-very few RNA molecules. The number of MAP2 molecules per particle is affected by synaptic activity and Staufen 2, indicating that RNP composition is tightly controlled. Our data suggest that the independent localization of individual RNAs in low copy numbers could contribute to tighter temporal and spatial control of expression in neurons and synapse-specific plasticity.",

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AB - Messenger RNA localization involves the assembly of ribonucleoprotein particles (RNPs) and their subsequent transport along the cytoskeleton to their final destination. Here, we provide new evidence that microtubule-associated protein 2 (MAP2), calcium/calmodulin-dependent protein kinase II (CaMKIIα) and β-actin RNAs localize to dendrites in distinct RNPs, which contain-unexpectedly-very few RNA molecules. The number of MAP2 molecules per particle is affected by synaptic activity and Staufen 2, indicating that RNP composition is tightly controlled. Our data suggest that the independent localization of individual RNAs in low copy numbers could contribute to tighter temporal and spatial control of expression in neurons and synapse-specific plasticity.

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Independent localization of MAP2, CaMKIIα and β-actin RNAs in low copy numbers

Abstract

Keywords

ASJC Scopus subject areas

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