Increased activity of cell membrane-associated prothrombinase, fibrinogen-like protein 2, in peripheral blood mononuclear cells of B-cell lymphoma patients

Esther Rabizadeh, Izhack Cherny, Ofir Wolach, Shany Sherman, Natalia Binkovski, Alon Peretz, Doron Lederfein, Aida Inbal

Research output: Contribution to journalArticlepeer-review

Abstract

Fibrinogen-like protein 2, FGL-2, was reported to be overexpressed in various cancer tissues, where it acts as a transmembrane prothrombinase. This study aims to determine the prothrombinase activity of FGL-2 in peripheral blood mononuclear cells (PBMC) of patients with B-cell lymphoma. FGL-2 activity was determined in patients with B-cell lymphoma (n = 53), and healthy controls (n = 145). FGL-2 activity in patients at diagnosis increased 3±0.3 fold (p<0.001). Sensitivity and specificity of the test was established at 73.6% and 80.7%, respectively, using a cutoff of 150% activity over control. Moreover, FGL-2 activity in 10 of 11 patients in remission decreased by 76%. In contrast, no significant difference was observed in expression levels of fgl-2 gene in patients and controls. Taken together, our study indicates that FGL-2 prothrombinase activity in PBMC of lymphoma patients is increased in active disease and normalizes during remission, thus being a potential marker for follow up of lymphoma patients.

Original languageEnglish
Article numbere109648
JournalPLoS ONE
Volume9
Issue number10
DOIs
StatePublished - 10 Oct 2014
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2014 Rabizadeh et al.

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