Expenditure on screening blood donations in developing countries can be reduced by testing donations in pools. This study evaluated serological screening in pools for hepatitis B virus (HBV) at the Israeli national blood bank and a hospital blood bank in Gaza, the Palestinian Authority. The accuracy of HBV surface antigen (HBsAg) enzyme immunoassay performed on pools of 3-24 samples was compared with individual tests. Delay in detecting positive samples due to dilution in pools and the possibility of antibody-antigen neutralization were analyzed. The sensitivity of pooled testing for HBsAg was 93-99%, prolonging the window period by 5 days (8.3%). Neutralization of HBsAg by hepatitis B surface antibodies (anti-HBs) could be minimized by testing immediately after pooling. Serological testing for HBsAg in pools may be performed using manually created pools of up to six samples, with 5% loss in sensitivity and a risk of neutralization by anti-HBs present in the donor population. Pooling can therefore be considered as an option only in countries with a low prevalence of HBV.
|Number of pages||6|
|Journal||Transactions of the Royal Society of Tropical Medicine and Hygiene|
|State||Published - Aug 2008|
Bibliographical noteFunding Information:
This research was supported by the U.S. Agency for International Development, Middle East Regional Cooperation (AID-MERC), Washington, DC, USA (project number M18-004).
- Blood donation
- Hepatitis B
- Hepatitis B virus surface antigen
- Sensitivity and specificity
ASJC Scopus subject areas
- Public Health, Environmental and Occupational Health
- Infectious Diseases