Impact of pooling on accuracy of hepatitis B virus surface antigen screening of blood donations

L. Novack; B. Sarov; R. Goldman-Levi; V. Yahalom; J. Safi; H. Soliman; M. Orgel; A. Yaari; N. Galai; J. S. Pliskin; E. Shinar

doi:10.1016/j.trstmh.2008.04.005

Impact of pooling on accuracy of hepatitis B virus surface antigen screening of blood donations

L. Novack, B. Sarov, R. Goldman-Levi, V. Yahalom, J. Safi, H. Soliman, M. Orgel, A. Yaari, N. Galai, J. S. Pliskin, E. Shinar

Department of Statistics

Research output: Contribution to journal › Article › peer-review

Abstract

Expenditure on screening blood donations in developing countries can be reduced by testing donations in pools. This study evaluated serological screening in pools for hepatitis B virus (HBV) at the Israeli national blood bank and a hospital blood bank in Gaza, the Palestinian Authority. The accuracy of HBV surface antigen (HBsAg) enzyme immunoassay performed on pools of 3-24 samples was compared with individual tests. Delay in detecting positive samples due to dilution in pools and the possibility of antibody-antigen neutralization were analyzed. The sensitivity of pooled testing for HBsAg was 93-99%, prolonging the window period by 5 days (8.3%). Neutralization of HBsAg by hepatitis B surface antibodies (anti-HBs) could be minimized by testing immediately after pooling. Serological testing for HBsAg in pools may be performed using manually created pools of up to six samples, with 5% loss in sensitivity and a risk of neutralization by anti-HBs present in the donor population. Pooling can therefore be considered as an option only in countries with a low prevalence of HBV.

Original language	English
Pages (from-to)	787-792
Number of pages	6
Journal	Transactions of the Royal Society of Tropical Medicine and Hygiene
Volume	102
Issue number	8
DOIs	https://doi.org/10.1016/j.trstmh.2008.04.005
State	Published - Aug 2008

Bibliographical note

Funding Information:
This research was supported by the U.S. Agency for International Development, Middle East Regional Cooperation (AID-MERC), Washington, DC, USA (project number M18-004).

Keywords

Antibody
Antigen
Blood donation
Hepatitis B
Hepatitis B virus surface antigen
Sensitivity and specificity

ASJC Scopus subject areas

Parasitology
Public Health, Environmental and Occupational Health
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.trstmh.2008.04.005

Cite this

Novack, L., Sarov, B., Goldman-Levi, R., Yahalom, V., Safi, J., Soliman, H., Orgel, M., Yaari, A., Galai, N., Pliskin, J. S., & Shinar, E. (2008). Impact of pooling on accuracy of hepatitis B virus surface antigen screening of blood donations. Transactions of the Royal Society of Tropical Medicine and Hygiene, 102(8), 787-792. https://doi.org/10.1016/j.trstmh.2008.04.005

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title = "Impact of pooling on accuracy of hepatitis B virus surface antigen screening of blood donations",

abstract = "Expenditure on screening blood donations in developing countries can be reduced by testing donations in pools. This study evaluated serological screening in pools for hepatitis B virus (HBV) at the Israeli national blood bank and a hospital blood bank in Gaza, the Palestinian Authority. The accuracy of HBV surface antigen (HBsAg) enzyme immunoassay performed on pools of 3-24 samples was compared with individual tests. Delay in detecting positive samples due to dilution in pools and the possibility of antibody-antigen neutralization were analyzed. The sensitivity of pooled testing for HBsAg was 93-99%, prolonging the window period by 5 days (8.3%). Neutralization of HBsAg by hepatitis B surface antibodies (anti-HBs) could be minimized by testing immediately after pooling. Serological testing for HBsAg in pools may be performed using manually created pools of up to six samples, with 5% loss in sensitivity and a risk of neutralization by anti-HBs present in the donor population. Pooling can therefore be considered as an option only in countries with a low prevalence of HBV.",

keywords = "Antibody, Antigen, Blood donation, Hepatitis B, Hepatitis B virus surface antigen, Sensitivity and specificity",

author = "L. Novack and B. Sarov and R. Goldman-Levi and V. Yahalom and J. Safi and H. Soliman and M. Orgel and A. Yaari and N. Galai and Pliskin, {J. S.} and E. Shinar",

note = "Funding Information: This research was supported by the U.S. Agency for International Development, Middle East Regional Cooperation (AID-MERC), Washington, DC, USA (project number M18-004). ",

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Novack, L, Sarov, B, Goldman-Levi, R, Yahalom, V, Safi, J, Soliman, H, Orgel, M, Yaari, A, Galai, N, Pliskin, JS & Shinar, E 2008, 'Impact of pooling on accuracy of hepatitis B virus surface antigen screening of blood donations', Transactions of the Royal Society of Tropical Medicine and Hygiene, vol. 102, no. 8, pp. 787-792. https://doi.org/10.1016/j.trstmh.2008.04.005

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T1 - Impact of pooling on accuracy of hepatitis B virus surface antigen screening of blood donations

AU - Novack, L.

AU - Sarov, B.

AU - Goldman-Levi, R.

AU - Yahalom, V.

AU - Safi, J.

AU - Soliman, H.

AU - Orgel, M.

AU - Yaari, A.

AU - Galai, N.

AU - Pliskin, J. S.

AU - Shinar, E.

N1 - Funding Information: This research was supported by the U.S. Agency for International Development, Middle East Regional Cooperation (AID-MERC), Washington, DC, USA (project number M18-004).

PY - 2008/8

Y1 - 2008/8

N2 - Expenditure on screening blood donations in developing countries can be reduced by testing donations in pools. This study evaluated serological screening in pools for hepatitis B virus (HBV) at the Israeli national blood bank and a hospital blood bank in Gaza, the Palestinian Authority. The accuracy of HBV surface antigen (HBsAg) enzyme immunoassay performed on pools of 3-24 samples was compared with individual tests. Delay in detecting positive samples due to dilution in pools and the possibility of antibody-antigen neutralization were analyzed. The sensitivity of pooled testing for HBsAg was 93-99%, prolonging the window period by 5 days (8.3%). Neutralization of HBsAg by hepatitis B surface antibodies (anti-HBs) could be minimized by testing immediately after pooling. Serological testing for HBsAg in pools may be performed using manually created pools of up to six samples, with 5% loss in sensitivity and a risk of neutralization by anti-HBs present in the donor population. Pooling can therefore be considered as an option only in countries with a low prevalence of HBV.

AB - Expenditure on screening blood donations in developing countries can be reduced by testing donations in pools. This study evaluated serological screening in pools for hepatitis B virus (HBV) at the Israeli national blood bank and a hospital blood bank in Gaza, the Palestinian Authority. The accuracy of HBV surface antigen (HBsAg) enzyme immunoassay performed on pools of 3-24 samples was compared with individual tests. Delay in detecting positive samples due to dilution in pools and the possibility of antibody-antigen neutralization were analyzed. The sensitivity of pooled testing for HBsAg was 93-99%, prolonging the window period by 5 days (8.3%). Neutralization of HBsAg by hepatitis B surface antibodies (anti-HBs) could be minimized by testing immediately after pooling. Serological testing for HBsAg in pools may be performed using manually created pools of up to six samples, with 5% loss in sensitivity and a risk of neutralization by anti-HBs present in the donor population. Pooling can therefore be considered as an option only in countries with a low prevalence of HBV.

KW - Antibody

KW - Antigen

KW - Blood donation

KW - Hepatitis B

KW - Hepatitis B virus surface antigen

KW - Sensitivity and specificity

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JF - Transactions of the Royal Society of Tropical Medicine and Hygiene

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ER -

Impact of pooling on accuracy of hepatitis B virus surface antigen screening of blood donations

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

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