Immunological and biological identification of tumour necrosis-like factor in sponges: Endotoxin that mediates necrosis formation in xenografts

Karin Pfeifer, Heinz C. Schröder, Baruch Rinkevich, Gerhard Uhlenbruck, Franz G. Hanisch, Branko Kurelec, Peter Scholz, Werner E.G. Müller

Research output: Contribution to journalArticlepeer-review

Abstract

Xenografts of the sponge Geodia cydonium in its closely related species G. rovinjensis resulted in a rapid rejection of the graft within a period of 5 days. We identified an immunoreactive tumour necrosis factor (TNF)-like activity in the xenograft (Mr of 30 000) two days after grafting. In-vivo injection of 5 μg human recombinant TNF-α induced cytotoxicity in sponge cells in the same pattern and time course as during natural xenograft rejection. Anti-TNF-α polyclonals were found to react with xenograft extracts, by Western blot analysis, as from day 2 after grafting. Using ELISA we detected the TNF-like activity from day 2 after grafting with peak levels at days 4 and 5, where the amount was 0.72 ng/μg tissue DNA. By day 1, gp27 (inhibitory aggregation factor) is already formed in the xenograft. In-vitro experiments on isolated G. cydonium cells showed that addition of purified gp27 induced the production of the TNF-like activity (up to 13.5 ng/ml). Evidence is presented that gp27 is a product of the gp180 lectin receptor. We conclude that gp27 induces TNF-like factor production, resulting in destruction and dissolution of the xenograft after 5 days.

Original languageEnglish
Pages (from-to)161-169
Number of pages9
JournalCytokine
Volume4
Issue number2
DOIs
StatePublished - Mar 1992
Externally publishedYes

Bibliographical note

Funding Information:
This work was supported by grants from Deutsche Forschungsgemeinschaft (SFB 169, A 11) and German-Israeli Foundation for Scientific Research & Development (no. I-154-034.11/90).

Keywords

  • endotoxin
  • Geodia cydonium
  • sponges
  • Tumour necrosis factor
  • xenograft

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Biochemistry
  • Molecular Biology
  • Hematology

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