Identification of the Endostyle as a Stem Cell Niche in a Colonial Chordate

Ayelet Voskoboynik; Yoav Soen; Yuval Rinkevich; Amalia Rosner; Hiroo Ueno; Ram Reshef; Katherine J. Ishizuka; Karla J. Palmeri; Elizabeth Moiseeva; Baruch Rinkevich; Irving L. Weissman

doi:10.1016/j.stem.2008.07.023

Identification of the Endostyle as a Stem Cell Niche in a Colonial Chordate

Ayelet Voskoboynik, Yoav Soen, Yuval Rinkevich, Amalia Rosner, Hiroo Ueno, Ram Reshef, Katherine J. Ishizuka, Karla J. Palmeri, Elizabeth Moiseeva, Baruch Rinkevich, Irving L. Weissman

Research output: Contribution to journal › Article › peer-review

Abstract

Stem cell populations exist in "niches" that hold them and regulate their fate decisions. Identification and characterization of these niches is essential for understanding stem cell maintenance and tissue regeneration. Here we report on the identification of a novel stem cell niche in Botryllus schlosseri, a colonial urochordate with high stem cell-mediated developmental activities. Using in vivo cell labeling, engraftment, confocal microscopy, and time-lapse imaging, we have identified cells with stemness capabilities in the anterior ventral region of the Botryllus' endostyle. These cells proliferate and migrate to regenerating organs in developing buds and buds of chimeric partners but do not contribute to the germ line. When cells are transplanted from the endostyle region, they contribute to tissue development and induce long-term chimerism in allogeneic tissues. In contrast, cells from other Botryllus' regions do not show comparable stemness capabilities. Cumulatively, these results define the Botryllus' endostyle region as an adult somatic stem cell niche.

Original language	English
Pages (from-to)	456-464
Number of pages	9
Journal	Cell Stem Cell
Volume	3
Issue number	4
DOIs	https://doi.org/10.1016/j.stem.2008.07.023
State	Published - 9 Oct 2008
Externally published	Yes

Bibliographical note

Funding Information:
We thank Amir Voskoboynik, T. Raveh, and A. De Tomaso for critical review, and P. Brown, R. Marinelli, R. Pesich L. Jerabek, C. Patton, K. Lee, and R. Will for generous support and helpful assistance. This study was supported by USPHS grant DK54762 and to a minor extent by HL58770 by a grant from the US-Israel Binational Science Foundation (2003-010) and by the Israel Science Foundation (project 550-06).

Keywords

STEMCELL

ASJC Scopus subject areas

Molecular Medicine
Genetics
Cell Biology

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10.1016/j.stem.2008.07.023

Cite this

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abstract = "Stem cell populations exist in {"}niches{"} that hold them and regulate their fate decisions. Identification and characterization of these niches is essential for understanding stem cell maintenance and tissue regeneration. Here we report on the identification of a novel stem cell niche in Botryllus schlosseri, a colonial urochordate with high stem cell-mediated developmental activities. Using in vivo cell labeling, engraftment, confocal microscopy, and time-lapse imaging, we have identified cells with stemness capabilities in the anterior ventral region of the Botryllus' endostyle. These cells proliferate and migrate to regenerating organs in developing buds and buds of chimeric partners but do not contribute to the germ line. When cells are transplanted from the endostyle region, they contribute to tissue development and induce long-term chimerism in allogeneic tissues. In contrast, cells from other Botryllus' regions do not show comparable stemness capabilities. Cumulatively, these results define the Botryllus' endostyle region as an adult somatic stem cell niche.",

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Identification of the Endostyle as a Stem Cell Niche in a Colonial Chordate

Abstract

Bibliographical note

Keywords

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