Identification and localization of a Rickettsia sp. in Bemisia tabaci (Homoptera: Aleyrodidae)

Yuval Gottlieb, Murad Ghanim, Elad Chiel, Dan Gerling, Vitaly Portnoy, Shimon Steinberg, Galil Tzuri, A. Rami Horowitz, Eduard Belausov, Neta Mozes-Daube, Svetlana Kontsedalov, Moshe Gershon, Shunit Gal, Nurit Katzir, Einat Zchori-Fein

Research output: Contribution to journalArticlepeer-review

Abstract

Whiteflies (Homoptera: Aleyrodidae) are sap-sucking insects that harbor "Candidatus Portiera aleyrodidarum," an obligatory symbiotic bacterium which is housed in a special organ called the bacteriome. These insects are also home for a diverse facultative microbial community which may include Hamiltonella, Arsenophonus, Fritchea, Wolbachia, and Cardinium spp. In this study, the bacteria associated with a B biotype of the sweet potato whitefly Bemisia tabaci were characterized using molecular fingerprinting techniques, and a Rickettsia sp. was detected for the first time in this insect family. Rickettsia sp. distribution, transmission and localization were studied using PCR and fluorescence in situ hybridizations (FISH). Rickettsia was found in all 20 Israeli B. tabaci populations screened but not in all individuals within each population. A FISH analysis of B. tabaci eggs, nymphs, and adults revealed a unique concentration of Rickettsia around the gut and follicle cells, as well as a random distribution in the hemolymph. We postulate that the Rickettsia enters the oocyte together with the bacteriocytes, leaves these symbiont-housing cells when the egg is laid, multiplies and spreads throughout the egg during embryogenesis and, subsequently, disperses throughout the body of the hatching nymph, excluding the bacteriomes. Although the role Rickettsia plays in the biology of the whitefly is currently unknown, the vertical transmission on the one hand and the partial within-population infection on the other suggest a phenotype that is advantageous under certain conditions but may be deleterious enough to prevent fixation under others.

Original languageEnglish
Pages (from-to)3646-3652
Number of pages7
JournalApplied and Environmental Microbiology
Volume72
Issue number5
DOIs
StatePublished - May 2006
Externally publishedYes

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Food Science
  • Biotechnology
  • Ecology

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