Histamine excites pedunculopontine neurones in guinea pig brainstem slices

A. Khateb, M. Serafin, M. Mühlethaler

Research output: Contribution to journalArticlepeer-review


Intracellular recordings were obtained from pedunculopontine tegmental nuclei neurones in guinea pig brainstem slices. These neurones were characterized by the presence of a slow regular firing (around 3 spikes/s), a broad action potential (more than 1 ms) and a transient rectification indicating the presence of an A current. Bath-application of histamine at 10-4 or 10-5 M induced a reversible increase in spontaneous firing. In presence of tetrodotoxine (1 μM), the effect of histamine was a reversible membrane depolarization. It was a direct effect as it persisted in presence of a low calcium/high magnesium solution. This excitatory action was presumably mediated by histamine H1 receptors as it could be blocked by the H1 receptor antagonist mepyramine but not by the H2 receptor antagonist cimetidine. A role in arousal is discussed.

Original languageEnglish
Pages (from-to)257-262
Number of pages6
JournalNeuroscience Letters
Issue number2-3
StatePublished - 4 May 1990
Externally publishedYes

Bibliographical note

Funding Information:
This work was supported by grants from the Swiss NSF ( and 3.560.0.86) and the Sandoz Foundation. We thank Ms. D. Machard for excellent technical assistance.


  • Arousal
  • Cholinergic nuclei
  • Histamine receptor
  • Reticular formation
  • Sleep-waking cycle

ASJC Scopus subject areas

  • General Neuroscience


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