Histamine excites pedunculopontine neurones in guinea pig brainstem slices

A. Khateb; M. Serafin; M. Mühlethaler

doi:10.1016/0304-3940(90)90213-S

Histamine excites pedunculopontine neurones in guinea pig brainstem slices

A. Khateb, M. Serafin, M. Mühlethaler

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Abstract

Intracellular recordings were obtained from pedunculopontine tegmental nuclei neurones in guinea pig brainstem slices. These neurones were characterized by the presence of a slow regular firing (around 3 spikes/s), a broad action potential (more than 1 ms) and a transient rectification indicating the presence of an A current. Bath-application of histamine at 10^-4 or 10^-5 M induced a reversible increase in spontaneous firing. In presence of tetrodotoxine (1 μM), the effect of histamine was a reversible membrane depolarization. It was a direct effect as it persisted in presence of a low calcium/high magnesium solution. This excitatory action was presumably mediated by histamine H₁ receptors as it could be blocked by the H₁ receptor antagonist mepyramine but not by the H₂ receptor antagonist cimetidine. A role in arousal is discussed.

Original language	English
Pages (from-to)	257-262
Number of pages	6
Journal	Neuroscience Letters
Volume	112
Issue number	2-3
DOIs	https://doi.org/10.1016/0304-3940(90)90213-S
State	Published - 4 May 1990
Externally published	Yes

Bibliographical note

Funding Information:
This work was supported by grants from the Swiss NSF (3.288.0.85 and 3.560.0.86) and the Sandoz Foundation. We thank Ms. D. Machard for excellent technical assistance.

Keywords

Arousal
Cholinergic nuclei
Histamine receptor
Reticular formation
Sleep-waking cycle

ASJC Scopus subject areas

General Neuroscience

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10.1016/0304-3940(90)90213-S

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title = "Histamine excites pedunculopontine neurones in guinea pig brainstem slices",

abstract = "Intracellular recordings were obtained from pedunculopontine tegmental nuclei neurones in guinea pig brainstem slices. These neurones were characterized by the presence of a slow regular firing (around 3 spikes/s), a broad action potential (more than 1 ms) and a transient rectification indicating the presence of an A current. Bath-application of histamine at 10-4 or 10-5 M induced a reversible increase in spontaneous firing. In presence of tetrodotoxine (1 μM), the effect of histamine was a reversible membrane depolarization. It was a direct effect as it persisted in presence of a low calcium/high magnesium solution. This excitatory action was presumably mediated by histamine H1 receptors as it could be blocked by the H1 receptor antagonist mepyramine but not by the H2 receptor antagonist cimetidine. A role in arousal is discussed.",

keywords = "Arousal, Cholinergic nuclei, Histamine receptor, Reticular formation, Sleep-waking cycle",

author = "A. Khateb and M. Serafin and M. M{\"u}hlethaler",

note = "Funding Information: This work was supported by grants from the Swiss NSF (3.288.0.85 and 3.560.0.86) and the Sandoz Foundation. We thank Ms. D. Machard for excellent technical assistance.",

year = "1990",

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doi = "10.1016/0304-3940(90)90213-S",

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AU - Khateb, A.

AU - Serafin, M.

AU - Mühlethaler, M.

N1 - Funding Information: This work was supported by grants from the Swiss NSF (3.288.0.85 and 3.560.0.86) and the Sandoz Foundation. We thank Ms. D. Machard for excellent technical assistance.

PY - 1990/5/4

Y1 - 1990/5/4

N2 - Intracellular recordings were obtained from pedunculopontine tegmental nuclei neurones in guinea pig brainstem slices. These neurones were characterized by the presence of a slow regular firing (around 3 spikes/s), a broad action potential (more than 1 ms) and a transient rectification indicating the presence of an A current. Bath-application of histamine at 10-4 or 10-5 M induced a reversible increase in spontaneous firing. In presence of tetrodotoxine (1 μM), the effect of histamine was a reversible membrane depolarization. It was a direct effect as it persisted in presence of a low calcium/high magnesium solution. This excitatory action was presumably mediated by histamine H1 receptors as it could be blocked by the H1 receptor antagonist mepyramine but not by the H2 receptor antagonist cimetidine. A role in arousal is discussed.

AB - Intracellular recordings were obtained from pedunculopontine tegmental nuclei neurones in guinea pig brainstem slices. These neurones were characterized by the presence of a slow regular firing (around 3 spikes/s), a broad action potential (more than 1 ms) and a transient rectification indicating the presence of an A current. Bath-application of histamine at 10-4 or 10-5 M induced a reversible increase in spontaneous firing. In presence of tetrodotoxine (1 μM), the effect of histamine was a reversible membrane depolarization. It was a direct effect as it persisted in presence of a low calcium/high magnesium solution. This excitatory action was presumably mediated by histamine H1 receptors as it could be blocked by the H1 receptor antagonist mepyramine but not by the H2 receptor antagonist cimetidine. A role in arousal is discussed.

KW - Arousal

KW - Cholinergic nuclei

KW - Histamine receptor

KW - Reticular formation

KW - Sleep-waking cycle

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SN - 0304-3940

VL - 112

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EP - 262

JO - Neuroscience Letters

JF - Neuroscience Letters

IS - 2-3

ER -

Histamine excites pedunculopontine neurones in guinea pig brainstem slices

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

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