Abstract
Two stripe-rust resistance genes, YrH52 and Yr15, derived from the Israeli wild emmer wheat, Triticum dicoccoides, have been located on chromosome 1B. The main objectives of the present study were to increase marker density in the vicinity of YrH52 gene by means of AFLP, RAPD and microsatellite markers, to improve the map of another T. dicoccoides-derived stripe-rust resistance gene Yr15 using microsatellite markers, and to preliminarily discriminate these two genes. Additional 26 marker loci comprising 20 AFLPs, three RAPDs, and three microsatellites were found to be linked to YrH52 gene. An updated genetic map consisting of 45 marker loci, in the region of YrH52 gene, was constructed with a total map length of 107.7cm. The mean interval length was 0.96 cm in the region Xgwm359b-P55M53b carrying YrH52 gene. YrH52 was bracketed by Xgwm413 (Nor1 and UBC212a) and Xgwm273a (Xgwm273d) with map distance of 1.3 and 2.7 cm from either side, respectively. Eight additional microsatellite markers were found to be linked with Yr15, and the linkage map of Yr15 gene was thus obviously improved. In the YrH52-mapping population, no crossover was detected in the interval UBC212a (Xgwm413)-Yr15-Nor1, and YrH52 was located distally outside this interval. It may suggest that YrH52 is different from Yr15 even though both of them are derived from T. dicoccoides and are mapped on chromosome 1BS. The large number of molecular makers revealed in the present study would be helpful for the marker-assisted introgression of the T. dicoccoides-derived YrH52 and Yr15 stripe-rust resistance genes into elite cultivars of wheat, and the high-density map would accelerate the map-based cloning of the two genes.
| Original language | English |
|---|---|
| Pages (from-to) | 199-210 |
| Number of pages | 12 |
| Journal | Genetica |
| Volume | 109 |
| Issue number | 3 |
| DOIs | |
| State | Published - Sep 2000 |
Bibliographical note
Funding Information:This work was supported by EMBO – European Molecular Biology Organization (Grant to the first author, No. ASTF9195), the Israel Discount Bank Chair of Evolutionary Biology, the Ancell-Teicher Research Foundation for Genetics and Molecular Evolution, the Israeli Ministry of Science (Grant No. 5757-1-95), an equipment grant of the Israel Science Foundation (Grant No. 9048/99), the German-Israeli Cooperation Project (DIP project funded by the BMBF and supported by BMBF’s International Bureau at the DLR), and the Graduate School of the University of Haifa, Israel. The authors are greatly indebted to Prof. A. B. Korol at the Institute of Evolution, University of Haifa, Israel for his comments and suggestions on the manuscript; to T. Krugman at the Institute of Evolution, University of Haifa, Israel for her assistance in laboratory and field experiments; and to K. Wendehake at the Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, Germany for her technical assistance in microsatellite analysis; to Dr. H. J. van Eck at the Department of Plant Breeding, Wageningen Agricultural University, the Netherlands for his kind help in AFLP analysis; to Dr. J. Manistersky and the late Prof. Z. Eyal at Tel Aviv University, Israel for kindly providing stripe-rust culture 5006; and to Dr. L. R. Joppa, Northern Crop Science Laboratory, State University Station, Fargo, ND 58105, USA for kindly providing the F1 seeds of our mapping population.
Keywords
- AFLP
- Microsatellite
- Molecular mapping
- Stripe-rust resistance gene
- Triticum dicoccoides
ASJC Scopus subject areas
- Animal Science and Zoology
- Genetics
- Plant Science
- Insect Science