Gram-negative outer-membrane proteins with multiple β-barrel domains

Ron Solan, Joana Pereira, Andrei N. Lupas, Rachel Kolodny, Nir Ben-Tal

Research output: Contribution to journalArticlepeer-review

Abstract

Outer-membrane beta barrels (OMBBs) are found in the outer membrane of gram-negative bacteria and eukaryotic organelles. OMBBs fold as antiparallel β-sheets that close onto themselves, forming pores that traverse the membrane. Currently known structures include only one barrel, of 8 to 36 strands, per chain. The lack of multi-OMBB chains is surprising, as most OMBBs form oligomers, and some function only in this state. Using a combination of sensitive sequence comparison methods and coevolutionary analysis tools, we identify many proteins combining multiple beta barrels within a single chain; combinations that include eight-stranded barrels prevail. These multibarrels seem to be the result of independent, lineage-specific fusion and amplification events. The absence of multibarrels that are universally conserved in bacteria with an outer membrane, coupled with their frequent de novo genesis, suggests that their functions are not essential but rather beneficial in specific environments. Adjacent barrels of complementary function within the same chain may allow for functions beyond those of the individual barrels.

Original languageEnglish
Article numbere2104059118
JournalProceedings of the National Academy of Sciences of the United States of America
Volume118
Issue number31
DOIs
StatePublished - 3 Aug 2021

Bibliographical note

Funding Information:
ACKNOWLEDGMENTS. This research has been supported by Grant 94747 by the Volkswagen Foundation. N.B.-T.’s research is supported in part by the Abraham E. Kazan Chair in Structural Biology, Tel Aviv University.

Publisher Copyright:
© 2021 National Academy of Sciences. All rights reserved.

Keywords

  • Coevolution analysis of bacterial sequences
  • Evolutionary analysis
  • Gram-negative bacteria
  • Outer-membrane beta barrels
  • Sequence analysis

ASJC Scopus subject areas

  • General

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