Glycerol-induced powdery mildew resistance in wheat by regulating plant fatty acid metabolism, plant hormones cross-talk, and pathogenesis-related genes

Yinghui Li; Lina Qiu; Xinye Liu; Qiang Zhang; Xiangxi Zhuansun; Tzion Fahima; Tamar Krugman; Qixin Sun; Chaojie Xie

doi:10.3390/ijms21020673

Glycerol-induced powdery mildew resistance in wheat by regulating plant fatty acid metabolism, plant hormones cross-talk, and pathogenesis-related genes

Yinghui Li, Lina Qiu, Xinye Liu, Qiang Zhang, Xiangxi Zhuansun, Tzion Fahima, Tamar Krugman, Qixin Sun, Chaojie Xie

Department of Evolutionary and Environmental Biology

Research output: Contribution to journal › Article › peer-review

Abstract

Our previous study indicated that glycerol application induced resistance to powdery mildew (Bgt) in wheat by regulating two important signal molecules, glycerol-3-phosphate (G3P) and oleic acid (OA18:1). Transcriptome analysis of wheat leaves treated by glycerol and inoculated with Bgt was performed to identify the activated immune response pathways. We identified a set of differentially expressed transcripts (e.g., TaGLI1, TaACT1, and TaSSI2) involved in glycerol and fatty acid metabolism that were upregulated in response to Bgt infection and might contribute to G3P and OA18:1 accumulation. Gene Ontology (GO) enrichment analysis revealed GO terms induced by glycerol, such as response to jasmonic acid (JA), defense response to bacterium, lipid oxidation, and growth. In addition, glycerol application induced genes (e.g., LOX, AOS, and OPRs) involved in the metabolism pathway of linolenic and alpha-linolenic acid, which are precursor molecules of JA biosynthesis. Glycerol induced JA and salicylic acid (SA) levels, while glycerol reduced the auxin (IAA) level in wheat. Glycerol treatment also induced pathogenesis related (PR) genes, including PR-1, PR-3, PR-10, callose synthase, PRMS, RPM1, peroxidase, HSP70, HSP90, etc. These results indicate that glycerol treatment regulates fatty acid metabolism and hormones cross-talk and induces the expression of PR genes that together contribute to Bgt resistance in wheat.

Original language	English
Article number	673
Journal	International Journal of Molecular Sciences
Volume	21
Issue number	2
DOIs	https://doi.org/10.3390/ijms21020673
State	Published - 20 Jan 2020

Bibliographical note

Funding Information:
Funding: This work was funded by the Natural Science Foundation of China (31671676), the National Key Research and Development Project of China (2016YFD0101802), and the Israel Science Foundation (1366/18).

Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.

Keywords

Disease resistance
Glycerol application
Transcriptome sequencing
Triticum aestivum L

ASJC Scopus subject areas

Catalysis
Molecular Biology
Spectroscopy
Computer Science Applications
Physical and Theoretical Chemistry
Organic Chemistry
Inorganic Chemistry

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10.3390/ijms21020673

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title = "Glycerol-induced powdery mildew resistance in wheat by regulating plant fatty acid metabolism, plant hormones cross-talk, and pathogenesis-related genes",

abstract = "Our previous study indicated that glycerol application induced resistance to powdery mildew (Bgt) in wheat by regulating two important signal molecules, glycerol-3-phosphate (G3P) and oleic acid (OA18:1). Transcriptome analysis of wheat leaves treated by glycerol and inoculated with Bgt was performed to identify the activated immune response pathways. We identified a set of differentially expressed transcripts (e.g., TaGLI1, TaACT1, and TaSSI2) involved in glycerol and fatty acid metabolism that were upregulated in response to Bgt infection and might contribute to G3P and OA18:1 accumulation. Gene Ontology (GO) enrichment analysis revealed GO terms induced by glycerol, such as response to jasmonic acid (JA), defense response to bacterium, lipid oxidation, and growth. In addition, glycerol application induced genes (e.g., LOX, AOS, and OPRs) involved in the metabolism pathway of linolenic and alpha-linolenic acid, which are precursor molecules of JA biosynthesis. Glycerol induced JA and salicylic acid (SA) levels, while glycerol reduced the auxin (IAA) level in wheat. Glycerol treatment also induced pathogenesis related (PR) genes, including PR-1, PR-3, PR-10, callose synthase, PRMS, RPM1, peroxidase, HSP70, HSP90, etc. These results indicate that glycerol treatment regulates fatty acid metabolism and hormones cross-talk and induces the expression of PR genes that together contribute to Bgt resistance in wheat.",

keywords = "Disease resistance, Glycerol application, Transcriptome sequencing, Triticum aestivum L",

author = "Yinghui Li and Lina Qiu and Xinye Liu and Qiang Zhang and Xiangxi Zhuansun and Tzion Fahima and Tamar Krugman and Qixin Sun and Chaojie Xie",

note = "Funding Information: Funding: This work was funded by the Natural Science Foundation of China (31671676), the National Key Research and Development Project of China (2016YFD0101802), and the Israel Science Foundation (1366/18). Publisher Copyright: {\textcopyright} 2020 by the authors. Licensee MDPI, Basel, Switzerland.",

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T1 - Glycerol-induced powdery mildew resistance in wheat by regulating plant fatty acid metabolism, plant hormones cross-talk, and pathogenesis-related genes

AU - Li, Yinghui

AU - Qiu, Lina

AU - Liu, Xinye

AU - Zhang, Qiang

AU - Zhuansun, Xiangxi

AU - Fahima, Tzion

AU - Krugman, Tamar

AU - Sun, Qixin

AU - Xie, Chaojie

N1 - Funding Information: Funding: This work was funded by the Natural Science Foundation of China (31671676), the National Key Research and Development Project of China (2016YFD0101802), and the Israel Science Foundation (1366/18). Publisher Copyright: © 2020 by the authors. Licensee MDPI, Basel, Switzerland.

PY - 2020/1/20

Y1 - 2020/1/20

N2 - Our previous study indicated that glycerol application induced resistance to powdery mildew (Bgt) in wheat by regulating two important signal molecules, glycerol-3-phosphate (G3P) and oleic acid (OA18:1). Transcriptome analysis of wheat leaves treated by glycerol and inoculated with Bgt was performed to identify the activated immune response pathways. We identified a set of differentially expressed transcripts (e.g., TaGLI1, TaACT1, and TaSSI2) involved in glycerol and fatty acid metabolism that were upregulated in response to Bgt infection and might contribute to G3P and OA18:1 accumulation. Gene Ontology (GO) enrichment analysis revealed GO terms induced by glycerol, such as response to jasmonic acid (JA), defense response to bacterium, lipid oxidation, and growth. In addition, glycerol application induced genes (e.g., LOX, AOS, and OPRs) involved in the metabolism pathway of linolenic and alpha-linolenic acid, which are precursor molecules of JA biosynthesis. Glycerol induced JA and salicylic acid (SA) levels, while glycerol reduced the auxin (IAA) level in wheat. Glycerol treatment also induced pathogenesis related (PR) genes, including PR-1, PR-3, PR-10, callose synthase, PRMS, RPM1, peroxidase, HSP70, HSP90, etc. These results indicate that glycerol treatment regulates fatty acid metabolism and hormones cross-talk and induces the expression of PR genes that together contribute to Bgt resistance in wheat.

AB - Our previous study indicated that glycerol application induced resistance to powdery mildew (Bgt) in wheat by regulating two important signal molecules, glycerol-3-phosphate (G3P) and oleic acid (OA18:1). Transcriptome analysis of wheat leaves treated by glycerol and inoculated with Bgt was performed to identify the activated immune response pathways. We identified a set of differentially expressed transcripts (e.g., TaGLI1, TaACT1, and TaSSI2) involved in glycerol and fatty acid metabolism that were upregulated in response to Bgt infection and might contribute to G3P and OA18:1 accumulation. Gene Ontology (GO) enrichment analysis revealed GO terms induced by glycerol, such as response to jasmonic acid (JA), defense response to bacterium, lipid oxidation, and growth. In addition, glycerol application induced genes (e.g., LOX, AOS, and OPRs) involved in the metabolism pathway of linolenic and alpha-linolenic acid, which are precursor molecules of JA biosynthesis. Glycerol induced JA and salicylic acid (SA) levels, while glycerol reduced the auxin (IAA) level in wheat. Glycerol treatment also induced pathogenesis related (PR) genes, including PR-1, PR-3, PR-10, callose synthase, PRMS, RPM1, peroxidase, HSP70, HSP90, etc. These results indicate that glycerol treatment regulates fatty acid metabolism and hormones cross-talk and induces the expression of PR genes that together contribute to Bgt resistance in wheat.

KW - Disease resistance

KW - Glycerol application

KW - Transcriptome sequencing

KW - Triticum aestivum L

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SN - 1661-6596

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JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

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ER -

Glycerol-induced powdery mildew resistance in wheat by regulating plant fatty acid metabolism, plant hormones cross-talk, and pathogenesis-related genes

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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