Genotype- and Cell-Specific Dynamics of Tandem Repeat Patterns in Aegilops speltoides Tausch (Poaceae, Triticeae)

Research output: Contribution to journalArticlepeer-review

Abstract

In wild plant populations, chromosome rearrangements lead to the wide intraspecific polymorphisms in the abundance and patterns of highly repetitive DNA. However, despite the large amount of accumulated data, the impact of the complex repetitive DNA fraction on genome reorganization and functioning and the mechanisms balancing and maintaining the structural integrity of the genome are not fully understood. Homologous recombination is thought to play a key role in both genome reshuffling and stabilization, while the contribution of nonhomologous recombination seems to be undervalued. Here, tandem repeat patterns and dynamics during pollen mother cell development were addressed, with a focus on the meiotic recombination that determines chromosome/genome repatterning and stabilization under cross-pollination and artificial hybridization in wild goatgrass, Aegilops speltoides. Native plants from contrasting allopatric populations and artificially created intraspecific hybrids were investigated using a FISH approach. Cytogenetic analysis uncovered a wide spectrum of genotype- and cell-specific chromosomal rearrangements, suggesting intensive repatterning of both parental and hybrid genomes. The data obtained provide evidence that repetitive elements serve as overabundant and ubiquitous resources for maintaining chromosome architecture/genome integrity through homologous and nonhomologous recombination at the intraorganismal level, and genotype-specific repatterning underlies intrapopulation polymorphisms and intraspecific diversification in the wild.

Original languageEnglish
Pages (from-to)105-116
Number of pages12
JournalCytogenetic and Genome Research
Volume153
Issue number2
DOIs
StatePublished - 1 Jan 2018

Bibliographical note

Publisher Copyright:
© 2018 S. Karger AG, Basel.

Keywords

  • Aegilops speltoides
  • Chromosomal rearrangements
  • Heterochromatin
  • Homologous recombination
  • Meiosis
  • Nonhomologous recombination
  • Repetitive DNA
  • Tandem repeats

ASJC Scopus subject areas

  • Genetics(clinical)
  • Genetics
  • Molecular Biology

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