Genome-wide identification, classification, evolutionary analysis and gene expression patterns of the protein kinase gene family in wheat and Aegilops tauschii

Jun Yan, Peisen Su, Zhaoran Wei, Eviatar Nevo, Lingrang Kong

Research output: Contribution to journalArticlepeer-review

Abstract

Key message: In this study we systematically identified and classified PKs in Triticum aestivum, Triticum urartu and Aegilops tauschii. Domain distribution and exon–intron structure analyses of PKs were performed, and we found conserved exon–intron structures within the exon phases in the kinase domain. Collinearity events were determined, and we identified various T. aestivum PKs from polyploidizations and tandem duplication events. Global expression pattern analysis of T. aestivum PKs revealed that some PKs might participate in the signaling pathways of stress response and developmental processes. QRT-PCR of 15 selected PKs were performed under drought treatment and with infection of Fusarium graminearum to validate the prediction of microarray. Abstract: The protein kinase (PK) gene superfamily is one of the largest families in plants and participates in various plant processes, including growth, development, and stress response. To better understand wheat PKs, we conducted genome-wide identification, classification, evolutionary analysis and expression profiles of wheat and Ae. tauschii PKs. We identified 3269, 1213 and 1448 typical PK genes in T. aestivum, T. urartu and Ae. tauschii, respectively, and classified them into major groups and subfamilies. Domain distributions and gene structures were analyzed and visualized. Some conserved intron–exon structures within the conserved kinase domain were found in T. aestivum, T. urartu and Ae. tauschii, as well as the primitive land plants Selaginella moellendorffii and Physcomitrella patens, revealing the important roles and conserved evolutionary history of these PKs. We analyzed the collinearity events of T. aestivum PKs and identified PKs from polyploidizations and tandem duplication events. Global expression pattern analysis of T. aestivum PKs revealed tissue-specific and stress-specific expression profiles, hinting that some wheat PKs may regulate abiotic and biotic stress response signaling pathways. QRT-PCR of 15 selected PKs were performed under drought treatment and with infection of F. graminearum to validate the prediction of microarray. Our results will provide the foundational information for further studies on the molecular functions of wheat PKs.

Original languageEnglish
Pages (from-to)227-242
Number of pages16
JournalPlant Molecular Biology
Volume95
Issue number3
DOIs
StatePublished - 1 Oct 2017

Bibliographical note

Funding Information:
Acknowledgements We would like to thank Prof. An-Yuan Guo (Huazhong University of Science and Technology) for advice on this study, Prof. Wei Wang (Shandong Agricultural University) for providing wheat seeds (DR0621 and DS0621), and Dr. D. Garvin (USDA-ARS in the USA) for providing wheat seeds (Apogee and Apogee73S2). This work was supported by the following funds to L.R.K: National Natural Science Foundation of China (31471488), NSFC 31520103911. This work was also supported by the funds to JY: Young Teacher innovation fund of Shandong Agricultural University (140-23848).

Publisher Copyright:
© 2017, Springer Science+Business Media B.V.

Keywords

  • Collinearity events
  • Conserved exon–intron structures
  • Expression pattern
  • Wheat protein kinase family

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

Fingerprint

Dive into the research topics of 'Genome-wide identification, classification, evolutionary analysis and gene expression patterns of the protein kinase gene family in wheat and Aegilops tauschii'. Together they form a unique fingerprint.

Cite this