Genome-wide analysis of mitochondrial DNA copy number reveals loci implicated in nucleotide metabolism, platelet activation, and megakaryocyte proliferation

R. J. Longchamps, S. Y. Yang, C. A. Castellani, W. Shi, J. Lane, M. L. Grove, T. M. Bartz, C. Sarnowski, C. Liu, K. Burrows, A. L. Guyatt, T. R. Gaunt, T. Kacprowski, J. Yang, P. L. De Jager, L. Yu, A. Bergman, R. Xia, M. Fornage, M. F. FeitosaM. K. Wojczynski, A. T. Kraja, M. A. Province, N. Amin, F. Rivadeneira, H. Tiemeier, A. G. Uitterlinden, L. Broer, J. B.J. Van Meurs, C. M. Van Duijn, L. M. Raffield, L. Lange, S. S. Rich, R. N. Lemaitre, M. O. Goodarzi, C. M. Sitlani, A. C.Y. Mak, D. A. Bennett, S. Rodriguez, J. M. Murabito, K. L. Lunetta, N. Sotoodehnia, G. Atzmon, K. Ye, N. Barzilai, J. A. Brody, B. M. Psaty, K. D. Taylor, J. I. Rotter, E. Boerwinkle, N. Pankratz, D. E. Arking

Research output: Contribution to journalArticlepeer-review

Abstract

Mitochondrial DNA copy number (mtDNA-CN) measured from blood specimens is a minimally invasive marker of mitochondrial function that exhibits both inter-individual and intercellular variation. To identify genes involved in regulating mitochondrial function, we performed a genome-wide association study (GWAS) in 465,809 White individuals from the Cohorts for Heart and Aging Research in Genomic Epidemiology (CHARGE) consortium and the UK Biobank (UKB). We identified 133 SNPs with statistically significant, independent effects associated with mtDNA-CN across 100 loci. A combination of fine-mapping, variant annotation, and co-localization analyses was used to prioritize genes within each of the 133 independent sites. Putative causal genes were enriched for known mitochondrial DNA depletion syndromes (p = 3.09 × 10–15) and the gene ontology (GO) terms for mtDNA metabolism (p = 1.43 × 10–8) and mtDNA replication (p = 1.2 × 10–7). A clustering approach leveraged pleiotropy between mtDNA-CN associated SNPs and 41 mtDNA-CN associated phenotypes to identify functional domains, revealing three distinct groups, including platelet activation, megakaryocyte proliferation, and mtDNA metabolism. Finally, using mitochondrial SNPs, we establish causal relationships between mitochondrial function and a variety of blood cell-related traits, kidney function, liver function and overall (p = 0.044) and non-cancer mortality (p = 6.56 × 10–4).

Original languageEnglish
Pages (from-to)127-146
Number of pages20
JournalHuman Genetics
Volume141
Issue number1
DOIs
StatePublished - Jan 2022

Bibliographical note

© 2021. The Author(s).

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Fingerprint

Dive into the research topics of 'Genome-wide analysis of mitochondrial DNA copy number reveals loci implicated in nucleotide metabolism, platelet activation, and megakaryocyte proliferation'. Together they form a unique fingerprint.

Cite this