Genome size variation of Lotus peregrinus at Evolution Canyon I microsite, lower Nahal Oren, Mt. Carmel, Israel

Nikol Gasmanová, Aleš Lebeda, Ivana Doležalova, Tova Cohen, Tomáš Pavlíček, Tzion Fahima, Eviatar Nevo

Research output: Contribution to journalArticlepeer-review

Abstract

On the basis of previous studies showing a positive correlation between number of copies of retrotransposons and geographical environment, we hypothesized that different ecogeographlcal conditions on opposite slopes of Evolution Canyon I could cause intraspecific variation in plant genome size. To test this hypothesis, we chose Lotus peregrlnus L. (annual, self-pollinator) as the first candidate because of Its biological contrast to the pre-viously studied carob tree (long-lived, cross-pollinator). Absolute nuclear DNA content of 60 genotypes of L. peregrtnus was estimated by PI flow cytometry, with tomato (Lycopersicon esculentum cv. Stupicke) as internal reference standard. The mean 2C-value in L. peregrlnus was 2.546 pg, ranging from 2.39 pg to 2.71 pg. The mean 2C-value was higher in plants from the south-facing slope (2.549 pg) than from the north-facing slope (2.544 pg), but we were not able to show significant interslope differences In genome size.

Original languageEnglish
Pages (from-to)39-46
Number of pages8
JournalActa Biologica Cracoviensia Series Botanica
Volume49
Issue number1
StatePublished - 2007

Keywords

  • Bird's-foot trefoil
  • Evolution Canyon
  • Flow cytometry
  • Genome size
  • Intraspeciflc variation
  • Lotus peregrinus
  • Nuclear DNA content
  • PI staining
  • Species plasticity

ASJC Scopus subject areas

  • Plant Science

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