Abstract
On the basis of previous studies showing a positive correlation between number of copies of retrotransposons and geographical environment, we hypothesized that different ecogeographlcal conditions on opposite slopes of Evolution Canyon I could cause intraspecific variation in plant genome size. To test this hypothesis, we chose Lotus peregrlnus L. (annual, self-pollinator) as the first candidate because of Its biological contrast to the pre-viously studied carob tree (long-lived, cross-pollinator). Absolute nuclear DNA content of 60 genotypes of L. peregrtnus was estimated by PI flow cytometry, with tomato (Lycopersicon esculentum cv. Stupicke) as internal reference standard. The mean 2C-value in L. peregrlnus was 2.546 pg, ranging from 2.39 pg to 2.71 pg. The mean 2C-value was higher in plants from the south-facing slope (2.549 pg) than from the north-facing slope (2.544 pg), but we were not able to show significant interslope differences In genome size.
Original language | English |
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Pages (from-to) | 39-46 |
Number of pages | 8 |
Journal | Acta Biologica Cracoviensia Series Botanica |
Volume | 49 |
Issue number | 1 |
State | Published - 2007 |
Keywords
- Bird's-foot trefoil
- Evolution Canyon
- Flow cytometry
- Genome size
- Intraspeciflc variation
- Lotus peregrinus
- Nuclear DNA content
- PI staining
- Species plasticity
ASJC Scopus subject areas
- Plant Science