Genetic structure of wild emmer wheat populations as reflected by transcribed versus anonymous SSR markers

Zvi Peleg, Tzion Fahima, Shahal Abbo, Tamar Krugman, Yehoshua Saranga

Research output: Contribution to journalArticlepeer-review

Abstract

Simple sequence repeat (SSR) markers have become a major tool in population genetic analyses. The anonymous genomic SSRs (gSSRs) have been recently supplemented with expressed sequence tag (EST) derived SSRs (eSSRs), which represent the transcribed regions of the genome. In the present study, we used 8 populations of wild emmer wheat (Triticum turgidum subsp. dicoccoides) to compare the usefulness of the two types of SSR markers in assessing allelic diversity and population structure. gSSRs revealed significantly higher diversity than eSSRs in terms of average number of alleles (14.92 vs. 7.4, respectively), polymorphic information content (0.87 vs. 0.68, respectively), and gene diversity (He; 0.55 vs. 0.38, respectively). Despite the overall differences in the level of diversity, Mantel tests for correlations between eSSR and gSSR pairwise genetic distances were found to be significant for each population as well as for all accessions jointly (RM = 0.54, p = 0.01). Various genetic structure analyses (AMOVA, PCoA, STRUCTURE, unrooted UPGMA tree) revealed a better capacity of eSSRs to distinguish between populations, while gSSRs showed a higher proportion of intra-population (among accessions) diversity. We conclude that eSSR and gSSR markers should be employed in conjunction to obtain a high inter- and intra-specific (or inter- and intra-varietal) distinctness.

Original languageEnglish
Pages (from-to)187-195
Number of pages9
JournalGenome
Volume51
Issue number3
DOIs
StatePublished - Mar 2008

Keywords

  • EST-SSR
  • Genomic SSR
  • Marker comparison
  • Population genetics
  • Population structure
  • Triticum turgidum subsp. dicoccoides

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Biotechnology

Fingerprint

Dive into the research topics of 'Genetic structure of wild emmer wheat populations as reflected by transcribed versus anonymous SSR markers'. Together they form a unique fingerprint.

Cite this