Genetic Removal of p70 S6 Kinase 1 Corrects Molecular, Synaptic, and Behavioral Phenotypes in Fragile X Syndrome Mice

Aditi Bhattacharya, Hanoch Kaphzan, Amanda C. Alvarez-Dieppa, Jaclyn P. Murphy, Philippe Pierre, Eric Klann

Research output: Contribution to journalArticlepeer-review

Abstract

The ability to chronically monitor neuronal activity in the living brain is essential for understanding the organization and function of the nervous system. The genetically encoded green fluorescent protein-based calcium sensor GCaMP provides a powerful tool for detecting calcium transients in neuronal somata, processes, and synapses that are triggered by neuronal activities. Here we report the generation and characterization of transgenic mice that express improved GCaMPs in various neuronal subpopulations under the control of the Thy1 promoter. In vitro and in vivo studies show that calcium transients induced by spontaneous and stimulus-evoked neuronal activities can be readily detected at the level of individual cells and synapses in acute brain slices, as well as chronically in awake, behaving animals. These GCaMP transgenic mice allow investigation of activity patterns in defined neuronal populations in the living brain and will greatly facilitate dissecting complex structural and functional relationships of neural networks.

Original languageEnglish
Pages (from-to)325-337
Number of pages13
JournalNeuron
Volume76
Issue number2
DOIs
StatePublished - 18 Oct 2012
Externally publishedYes

Bibliographical note

Funding Information:
This work was supported by NIH grants NS034007 and NS047384 (E.K.), FRAXA Research Foundation (E.K.), and a FRAXA Postdoctoral Fellowship (A.B). J.P.M. was supported by a summer training grant, NSF REU Site Grant in Neural Science DBI 1004172.

Keywords

  • MENTAL-RETARDATION PROTEIN
  • LONG-TERM DEPRESSIONMR1 KNOCKOUT MOUSEMESSENGER-RNAMAMMALIAN TARGETTRANSLATIONMTORMEMORY
  • PHOSPHOINOSITIDE 3-KINASE-AKT-MAMMALIAN TARGET
  • RAPAMYCIN SIGNALING PATHWAY
  • FMR1 KNOCKOUT MOUSE
  • MESSENGER-RNA
  • MAMMALIAN TARGET
  • TRANSLATION
  • MTOR
  • MEMORY

ASJC Scopus subject areas

  • General Neuroscience

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