Genetic, immunofl uorescence labeling, and in situ hybridization techniques in identifi cation of stem cells in male and female germline niches

Shree Ram Singh, Ying Liu, Madhuri Kango-Singh, Eviatar Nevo

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

Stem cells have an enormous capacity of self-renewal, as well as the ability to differentiate into specialized cell types. Proper control of these two properties of stem cells is crucial for animal development, growth control, and reproduction. Germline stem cells (GSCs) are a self-renewing population of germ cells, which generate haploid gametes (sperms or oocyte) that transmit genetic information from generation to generation. In Drosophila testis and ovary, GSCs are anchored around the niche cells. The cap cells cluster in females and hub cells in males act as a niche to control GSC behavior. With highly sophisticated genetic techniques in Drosophila , tremendous progress has been made in understanding the interactions between stem cells and niches at cellular and molecular levels. Here, we provide details of genetic, immunofl uorescence labeling, and in situ hybridization techniques in identifi cation and characterization of stem cells in Drosophila male and female germline niches.

Original languageEnglish
Title of host publicationStem Cell Niche
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages9-23
Number of pages15
ISBN (Print)9781627035071
DOIs
StatePublished - 2013

Publication series

NameMethods in Molecular Biology
Volume1035
ISSN (Print)1064-3745

Keywords

  • Drosophila
  • Germline stem cells
  • Niches
  • Ovary
  • Testis

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology

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