Genetic effects on microsatellite diversity in wild emmer wheat (Triticum dicoccoides) at the Yehudiyya microsite, Israel

Y. C. Li; T. Fahima; M. S. Röder; V. M. Kirzhner; A. Beiles; A. B. Korol; E. Nevo

doi:10.1038/sj.hdy.6800190

Genetic effects on microsatellite diversity in wild emmer wheat (Triticum dicoccoides) at the Yehudiyya microsite, Israel

Y. C. Li, T. Fahima, M. S. Röder, V. M. Kirzhner, A. Beiles, A. B. Korol, E. Nevo

Department of Evolutionary and Environmental Biology

Research output: Contribution to journal › Article › peer-review

Abstract

This study investigated allele size constraints and clustering, and genetic effects on microsatellite (simple sequence repeat, SSR) diversity at 28 loci comprising seven types of tandem repeated dinucleotide motifs in a natural population of wild emmer wheat, Triticum dicoccoides, from a shade vs sun microsite in Yehudiyya, northeast of the Sea of Galilee, Israel. It was found that allele distribution at SSR loci is clustered and constrained with lower or higher boundary. This may imply that SSR have functional significance and natural constraints. Genetic factors, involving genome, chromosome, motif, and locus significantly affected SSR diversity. Genome B appeared to have a larger average repeat number (ARN), but lower variance in repeat number (σ_ARN²), and smaller number of alleles per locus than genome A. SSRs with compound motifs showed larger ARN than those with perfect motifs. The effects of replication slippage and recombinational effects (eg, unequal crossing over) on SSR diversity varied with SSR motifs. Ecological stresses (sun vs shade) may affect mutational mechanisms, influencing the level of SSR diversity by both processes.

Original language	English
Pages (from-to)	150-156
Number of pages	7
Journal	Heredity
Volume	90
Issue number	2
DOIs	https://doi.org/10.1038/sj.hdy.6800190
State	Published - 1 Feb 2003

Bibliographical note

Funding Information:
The authors thank Dr V Korzun and Ms K Wendehake for their excellent help, and the Graduate School, University of Haifa for financial support to this study whose microsatellite work was conducted in Germany at the laboratory of the Institute for Plant Genetics and Crop Plant Research. This research is part of the PhD research project of the first author. The research was financially supported by an equipment grant of The Israel Science Foundation (No. 9048/99); and by the German–Israeli Cooperation Project (DIP project funded by the BMBF and supported by BMBF’s International Bureau at the DLR). This work was also supported by the Israeli Discount Bank Chair of Evolutionary Biology and the Ancell–Teicher Research Foundation for Genetics and Molecular Evolution.

Keywords

Allelic cluster
Genome effect
Mutational mechanism
SSR variation
Triticum dicoccoides
Wheat's progenitor

ASJC Scopus subject areas

Genetics
Genetics(clinical)

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10.1038/sj.hdy.6800190

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title = "Genetic effects on microsatellite diversity in wild emmer wheat (Triticum dicoccoides) at the Yehudiyya microsite, Israel",

abstract = "This study investigated allele size constraints and clustering, and genetic effects on microsatellite (simple sequence repeat, SSR) diversity at 28 loci comprising seven types of tandem repeated dinucleotide motifs in a natural population of wild emmer wheat, Triticum dicoccoides, from a shade vs sun microsite in Yehudiyya, northeast of the Sea of Galilee, Israel. It was found that allele distribution at SSR loci is clustered and constrained with lower or higher boundary. This may imply that SSR have functional significance and natural constraints. Genetic factors, involving genome, chromosome, motif, and locus significantly affected SSR diversity. Genome B appeared to have a larger average repeat number (ARN), but lower variance in repeat number (σARN2), and smaller number of alleles per locus than genome A. SSRs with compound motifs showed larger ARN than those with perfect motifs. The effects of replication slippage and recombinational effects (eg, unequal crossing over) on SSR diversity varied with SSR motifs. Ecological stresses (sun vs shade) may affect mutational mechanisms, influencing the level of SSR diversity by both processes.",

keywords = "Allelic cluster, Genome effect, Mutational mechanism, SSR variation, Triticum dicoccoides, Wheat's progenitor",

author = "Li, {Y. C.} and T. Fahima and R{\"o}der, {M. S.} and Kirzhner, {V. M.} and A. Beiles and Korol, {A. B.} and E. Nevo",

note = "Funding Information: The authors thank Dr V Korzun and Ms K Wendehake for their excellent help, and the Graduate School, University of Haifa for financial support to this study whose microsatellite work was conducted in Germany at the laboratory of the Institute for Plant Genetics and Crop Plant Research. This research is part of the PhD research project of the first author. The research was financially supported by an equipment grant of The Israel Science Foundation (No. 9048/99); and by the German–Israeli Cooperation Project (DIP project funded by the BMBF and supported by BMBF{\textquoteright}s International Bureau at the DLR). This work was also supported by the Israeli Discount Bank Chair of Evolutionary Biology and the Ancell–Teicher Research Foundation for Genetics and Molecular Evolution.",

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AU - Kirzhner, V. M.

AU - Beiles, A.

AU - Korol, A. B.

AU - Nevo, E.

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N2 - This study investigated allele size constraints and clustering, and genetic effects on microsatellite (simple sequence repeat, SSR) diversity at 28 loci comprising seven types of tandem repeated dinucleotide motifs in a natural population of wild emmer wheat, Triticum dicoccoides, from a shade vs sun microsite in Yehudiyya, northeast of the Sea of Galilee, Israel. It was found that allele distribution at SSR loci is clustered and constrained with lower or higher boundary. This may imply that SSR have functional significance and natural constraints. Genetic factors, involving genome, chromosome, motif, and locus significantly affected SSR diversity. Genome B appeared to have a larger average repeat number (ARN), but lower variance in repeat number (σARN2), and smaller number of alleles per locus than genome A. SSRs with compound motifs showed larger ARN than those with perfect motifs. The effects of replication slippage and recombinational effects (eg, unequal crossing over) on SSR diversity varied with SSR motifs. Ecological stresses (sun vs shade) may affect mutational mechanisms, influencing the level of SSR diversity by both processes.

AB - This study investigated allele size constraints and clustering, and genetic effects on microsatellite (simple sequence repeat, SSR) diversity at 28 loci comprising seven types of tandem repeated dinucleotide motifs in a natural population of wild emmer wheat, Triticum dicoccoides, from a shade vs sun microsite in Yehudiyya, northeast of the Sea of Galilee, Israel. It was found that allele distribution at SSR loci is clustered and constrained with lower or higher boundary. This may imply that SSR have functional significance and natural constraints. Genetic factors, involving genome, chromosome, motif, and locus significantly affected SSR diversity. Genome B appeared to have a larger average repeat number (ARN), but lower variance in repeat number (σARN2), and smaller number of alleles per locus than genome A. SSRs with compound motifs showed larger ARN than those with perfect motifs. The effects of replication slippage and recombinational effects (eg, unequal crossing over) on SSR diversity varied with SSR motifs. Ecological stresses (sun vs shade) may affect mutational mechanisms, influencing the level of SSR diversity by both processes.

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KW - Mutational mechanism

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KW - Wheat's progenitor

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ER -

Genetic effects on microsatellite diversity in wild emmer wheat (Triticum dicoccoides) at the Yehudiyya microsite, Israel

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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